Evaluation of the Impact of the Trivedi Effect<sup>®</sup> -Energy of Consciousness on the Structure and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy

Abstract

Magnesium gluconate is a classical pharmaceutical/nutraceutical compound used as a magnesium ion source for the prevention and treatment of hypomagnesemia. The present study was aimed to investigate the effect of The Trivedi Effect ® -Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (P M+1 /P M and P M+2 /P M ) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts -one part was control, and another part was treated with The Trivedi Effect ® -Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect ® Treated sample. The LC-MS analysis of both the control and treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of the both sample were almost similar. The relative peak intensities of the fragment ions were significantly changed in the treated sample compared with the control sample. The proton and carbon signals for CH, CH 2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. + to m/z 417 in treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect ® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect ® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through American Journal of Biomedical and Life Sciences 2017; 5(1): 6-15 7 its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.