Evaluation of the impact of tromethamine edetate disodium dihydrate on antimicrobial susceptibility of <i><scp>P</scp>seudomonas aeruginosa</i> in biofilm <i>in vitro</i>

Pye, Charlotte; Singh, Ameet; Weese, J. Scott

doi:10.1111/vde.12115

Cited by 16 publications

(16 citation statements)

References 28 publications

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“…neomycin, streptomycin, kanamycin, spectinomycin, gentamicin and tobramycin), erythromycin and imipenem can lead to antagonism against various bacterial strains, including P. aeruginosa (Goswami & Jawali, ; Parry & Neu, ; Rodríguez‐Beltrán et al, ; Saggers & Lawson, ). Likewise, when Tris‐EDTA combined with enrofloxacin against P. aeruginosa canine otic isolates ( n = 31), there was a reduction in antibacterial efficacy with fourfold or higher MIC in 39% of the isolates (Pye et al, ). However, we have shown in a previous study (in press) that such antagonism does not occur when NAC or Tris‐EDTA is combined with ionophores (Chan et al, in press).…”

Section: Discussionmentioning

confidence: 99%

“…Antibiofilm activities of commercial antiseptics such as Octenillin ® , Prontosan ® and Betadine ® against S. aureus and P. aeruginosa biofilms increased by 20,000‐fold, 4000‐fold and 200‐fold, respectively in the presence of EDTA (Lefebvre, Vighetto, Di Martino, Garde, & Seyer, ). Pye, Singh, and Weese () demonstrated that Triz‐EDTA ® aqueous flush increased the susceptibility of P. aeruginosa biofilms (MIC 90 ) to gentamicin by 16‐fold and neomycin by twofold in cases of canine OE. Hence, further study is warranted to test the combination of Tris‐EDTA or disodium EDTA with other antimicrobial agents for the presence of synergistic antibiofilm activity.…”

Section: Discussionmentioning

confidence: 99%

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Biofilm production by pathogens associated with canine otitis externa, and the antibiofilm activity of ionophores and antimicrobial adjuvants

Chan

Hickey

Page³

et al. 2019

Vet Pharm & Therapeutics

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Otitis externa (OE) is a frequently reported disorder in dogs associated with secondary infections by Staphylococcus, Pseudomonas and yeast pathogens. The presence of biofilms may play an important role in the resistance of otic pathogens to antimicrobial agents. Biofilm production of twenty Staphylococcus pseudintermedius and twenty Pseudomonas aeruginosa canine otic isolates was determined quantitatively using a microtiter plate assay, and each isolate was classified as a strong, moderate, weak or nonbiofilm producer. Minimum biofilm eradication concentration (MBEC) of two ionophores (narasin and monensin) and three adjuvants (N‐acetylcysteine (NAC), Tris‐EDTA and disodium EDTA) were investigated spectrophotometrically (OD570nm) and quantitatively (CFU/ml) against selected Staphylococcus and Pseudomonas biofilm cultures. Concurrently, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of planktonic cultures were assessed. 16/20 of the S. pseudintermedius clinical isolates were weak biofilm producers. 19/20 P. aeruginosa clinical isolates produced biofilms and were distributed almost equally as weak, moderate and strong biofilm producers. While significant antibiofilm activity was observed, no MBEC was achieved with narasin or monensin. The MBEC for NAC ranged from 5,000–10,000 µg/ml and from 20,000–80,000 µg/ml against S. pseudintermedius and P. aeruginosa, respectively. Tris‐EDTA eradicated P. aeruginosa biofilms at concentrations ranging from 6,000/1,900 to 12,000/3,800 µg/ml. The MBEC was up to 16‐fold and eightfold higher than the MIC/MBC of NAC and Tris‐EDTA, respectively. Disodium EDTA reduced biofilm growth of both strains at concentrations of 470 µg/ml and higher. It can be concluded that biofilm production is common in pathogens associated with canine OE. NAC and Tris‐EDTA are effective antibiofilm agents in vitro that could be considered for the treatment of biofilm‐associated OE in dogs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Biofilm production by pathogens associated with canine otitis externa, and the antibiofilm activity of ionophores and antimicrobial adjuvants

Chan

Hickey

Page³

et al. 2019

Vet Pharm & Therapeutics

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“…Otitis is believed to be multifactorial and treatment can be difficult in some cases. [1][2][3] Common organisms isolated from dogs with otitis externa include Staphylococcus spp., Pseudomonas spp., Proteus spp., Streptococcus spp., Escherichia coli, Klebsiella spp., Bacteroides spp., Pasteurella spp. and Malassezia spp.…”

Section: Introductionmentioning

confidence: 99%

In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris‐EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis

Ghibaudo¹,

Santospirito

Sala

et al. 2016

Veterinary Dermatology

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Background -Pseudomonas aeruginosa (PA) may cause suppurative otitis externa with severe inflammation and ulceration in dogs. Multidrug resistance is commonly reported for this organism, creating a difficult therapeutic challenge.Objective -The aim of this study was to evaluate the in vitro antimicrobial activity of a gel containing 0.5 lg/mL of antimicrobial peptide AMP2041, 0.07% chlorhexidine digluconate (CLX), 0.4% Tris and 0.1% EDTA on 30 clinical isolates of PA from canine otitis externa.Materials and Methods -Antimicrobial activity was evaluated through minimal bactericidal concentration (MBC). Standardized bacterial suspensions were incubated with different concentrations of the gel at 37°C for 30 min and plated for colony forming unit (CFU) counts. Time-to-kill kinetics were evaluated with the undiluted product and at MBC for each PA strain at 30 s, 1, 5, 10, 15, 30 min, 24 and 48 h.Results -The MBC was 1:64 for two of 30 strains, 1:128 for 15 of 30 strains and 1:256 for 13 of 30 strains. The geometric mean was 1:165, equivalent to a concentration of 0.003 lg/mL AMP2041 + 0.0004% CLX + 0.0024% Tris + 0.0006% EDTA. Time-to-kill assays with the undiluted product showed complete bactericidal effect within 30 s for all isolates, whereas at the MBC this effect was reached within 5 min for 20 of 30 isolates and within 30 min for all isolates. Bactericidal activity was maintained after 48 h for all isolates.Conclusion -This gel has shown rapid, complete and long-lasting activity against a panel of 30 PA isolates from cases of canine otitis externa.

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“…As for carbapenems, it is rather striking to detect 2% resistance in reptile strains as their canine and feline parallels were all sensitive according to current literature [8]. Although polymyxin B resistance was once reported in canine P. aeruginosa [9], there exists no direct evidence to implicate colistin (also known as polymyxin E) resistance that was expressed by almost 50% of snake isolates in our study. Combined, to our knowledge, P. aeruginosa from snakes' oral cavity are highly antibiotic-resistant.…”

Section: Discussionmentioning

confidence: 57%

Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan

Liu

Ling-ling

Tseng

et al. 2017

Canadian Journal of Infectious Diseases and Medical Microbiolog

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This study included fifty-eight isolates of P. aeruginosa from the oral cavity of snakes that were recruited from clinical cases, captive and wild snakes. The minimum inhibitory concentrations (MICs) for the determination of susceptibility were identified by the broth microdilution method. Polymerase chain reaction (PCR) was employed to detect β-lactamases genes. With regard to antipseudomonal antibiotics, the lowest nonsusceptible rates were in aztreonam (15%), piperacillin/tazobactam (12%), and amikacin (9%). The nonsusceptible rates were high in gentamicin (33%) and colistin (55%). Meanwhile, blaTEM presented in 100% of isolates where blaAmpC, blaOXA-1, and blaOXA-10 came at 94.8%, 89.7%, and 27.6%, respectively. Emergence of multidrug resistant (MDR) strains and colistin-resistant strains highlights the potential breach of public health as P. aeruginosa could be transmitted through either direct contact or indirect dissemination through the environment. This study reports that the highly resistant P. aeruginosa from snakes' oral cavity were discovered for the very first time in Taiwan.

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Evaluation of the impact of tromethamine edetate disodium dihydrate on antimicrobial susceptibility of Pseudomonas aeruginosa in biofilm in vitro

Abstract: Triz-EDTA(®) may be a useful adjunctive treatment for chronic cases of Pseudomonas otitis where biofilms may have developed, if gentamicin or neomycin is to be used as a topical treatment. In vivo study is required to confirm this effect.

Cited by 16 publications

References 28 publications

Biofilm production by pathogens associated with canine otitis externa, and the antibiofilm activity of ionophores and antimicrobial adjuvants

Biofilm production by pathogens associated with canine otitis externa, and the antibiofilm activity of ionophores and antimicrobial adjuvants

In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris‐EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis

Colistin Resistance of Pseudomonas aeruginosa Isolated from Snakes in Taiwan

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