Evaluation of the immunoradiometric and electrochemiluminescence method for the measurement of serum insulin in children

Abstract

Human insulin is a polypeptide hormone produced, stored, and secreted by the ß-cells in the pancreatic islets of Langerhans. Its secretion is stimulated by an increase of the glucose concentration in circulation. Non-radioactive assays are frequently used in many laboratories to measure hormone concentrations, as an alternative to the traditional "gold standard" radioimmuno- and immunoradiometric assays. The precise and reliable determination of the insulin concentration is an important concern in numerous diagnostic procedures. The aim of this study was to compare two commercially available assays (manual and automated) for measurement of serum insulin concentrations. Aliquots of the 86 randomly selected serum samples were measured by Elecsys Insulin Assay (cobas e411 immunoassay analyzer, Roche Diagnostics GmbH, Mannheim, Germany) and DIAsource INS-IRMA Kit (DIAsource ImmunoAssays S.A., Louvain-la-Neuve, Belgium). Compared assays exhibit good correlation (r = 0.996). Insulin concentrations were on average 4.2 μ IU/mL lower (p < 0.05) with the cobas e411 immunoassay analyzer when compared to those measured with DIAsouce Immunoassay. Our findings suggest that electrochemiluminescence method on the cobas e411 analyzer and manual IRMA method offered by the DIAsource for the serum insulin determination could be considered interchangeable.

Effect of plane of nutrition during the first 12 weeks of life on growth, metabolic and reproductive hormone concentrations, and testicular relative mRNA abundance in preweaned Holstein Friesian bull calves

“…Concentrations of the metabolic hormones, insulin and IGF-1, were determined through immunoradiometric assay (IRMA) and radioimmunoassay (RIA), respectively. Specifically, insulin concentrations were determined through IRMA as previously described by Ochocińska et al (2016) (DIAsource Immunoassays SA, Louvain-la-Neuve, Belgium). Insulin assay sensitivity was 4.56 ng/mL, while intra-assay CVs for low, medium, and high standards were 10.13%, 9.79%, and 8.11%, respectively.…”

Role of early life nutrition on the hypothalamic-pituitary-testicular axis of the bull

“…The sensitivity of IGF-1 assay was 4 ng/mL. Insulin concentrations were measured by immunoradiometric assay (IRMA; INS-Irma, DIAsource ImmunoAssays SA, Louvain-la-Neuve, Belgium) as described by Ochocińska et al (2016). The inter-and intra-assay CVs for the low, medium and high insulin standards were for 0.09, 7.16 and 2.93% and 6.58, 9.23 and 6.62% respectively.…”

Clinical Utility and Cross-Reactivity of Insulin and C-Peptide Assays by the Lumipulse G1200 System

“…One recent study has shown commutability of insulin immunoassay results using reference materials [ 21 ]. It may be helpful to establish the basic accuracy of the calibrator using standard controls to compare different methods [ 8 22 ].…”

“…Absolute values of the Lumipulse G1200 system were lower in diabetic patients as well as in healthy subjects. The source of discrepant results among the two insulin immunoassays is likely to be multifactorial, including antibody specificity, assay performance, clinical characteristics, and calibration procedures [ 8 21 23 ]. Although there was a slight difference in the absolute values of insulin and C-peptide concentrations between the two systems, it was not statistically significant (95% CI: −36.8–75.0), and the precision values were acceptable.…”