Evaluation of the immunogenicity of Schistosoma mansoni egg surface

Abstract

Introduction: Immunogenicity of Schistosoma mansoni egg surface was examined to determine whether intact eggshells have lower antigenicity than ruptured eggs. Methods: Swiss Webster mice were inoculated with intact or ultrasonicated S. mansoni eggs isolated from infected human feces. Mice were separated into four groups of six animals each and immunizations were performed approximately every 20 days during a 60-day period. Groups 1-4 were administered with saline solution, sonicated eggs with Freund's adjuvant, sonicated eggs without Freund's adjuvant, and intact eggs, respectively. IgG humoral immune response was assessed by ELISA using Soluble Egg Antigen produced from eggs isolated from the livers of infected mice. Results: Sonicated eggs co-administered with adjuvant induced the highest humoral response at 58 days, which was 11.9-fold (95% CI 6.2-17.5) greater than the response induced by saline solution. Sonicated eggs without adjuvant induced a 4.3-fold stronger response (95% CI 2.4-6.2) than normal saline. Intact eggs induced humoral response that was nominally twice stronger (95% CI 0.8-3.2) than that induced by normal saline but the effect did not reach statistical significance. Conclusions: Soluble antigens are not abundant on the surface of S. mansoni eggs and/or are not secreted in sufficient quantities to induce a significant immune response to intact eggs. Assuming that isolation procedures had not damaged the eggs used for inoculation, our observations suggest that intact eggs either do not induce a significant immune response or, if they do, the mechanism involves insoluble antigens from the egg surface.

Indicadores De Prevalência Em Tentativas De Suicídio Por Intoxicação De Medicamentos. Uma Questão De Saúde Pública

“…Maior parte das causas de morbidade provocada pela esquistossomose crônica são os ovos e as respostas imunológicas do parasita que eles evocam. Os pares de vermes do Schistosoma mansoni ficam em torno de 350 ovos por dia (CANDIDO et al, 2017).…”

Efeito Do Tratamento Da Auriculoterapia Na Percepção De Pacientes Com Cervicalgia Crônica

“…Maior parte das causas de morbidade provocada pela esquistossomose crônica são os ovos e as respostas imunológicas do parasita que eles evocam. Os pares de vermes do Schistosoma mansoni ficam em torno de 350 ovos por dia (CANDIDO et al, 2017).…”

The Protective Role of Toll-like Receptors Agonist Monophosphoryl Lipid A against Vaccinated Murine Schistosomiasis

“…The combination of protection using SEA and adjuvant was recommended in several studies [25][26][27][28][29] as it provided many complementary goals, a reduction of egg-induced pathology, minimal parenchymal changes and the eradication of worms. Therefore, the assessment of the effect of MPLA adjuvant with protective antigen SEA against infected mice is important by studying several criteria related to the parasitic intensity, stages and distribution through the tissues of the host for the evaluation of the magnitude of infection and efficacy of the treatment [30].…”