Evaluation of the Immunochromatographic NG-Test Carba 5, RESIST-5 O.O.K.N.V., and IMP K-SeT for Rapid Detection of KPC-, NDM-, IMP-, VIM-type, and OXA-48-like Carbapenemase Among Enterobacterales

Abstract

BackgroundEnterobacterales are the most common pathogens for nosocomial infections. The emergence and spread of KPC, NDM, and OXA-48-like carbapenemase-producing Enterobacterales with their extensively drug-resistant characteristics have posed great threats to public health. This study aimed to evaluate the performance of NG-test Carba 5, RESIST-5 O.O.K.N.V., and IMP K-SeT for rapid detection of five carbapenemases (KPC, NDM, VIM, IMP, and OXA-48-like) among Enterobacterales.MethodsA total of 186 carbapenem-resistant Enterobacterales clinical isolates and 29 reference strains were used in this study. Carbapenemase genes were confirmed by PCR and DNA sequencing. The sensitivities and specificities of these assays were calculated utilizing the VassarStats software.ResultsFor clinical isolates, the NG-test Carba 5 detected KPC, NDM, OXA-48-like, IMP, and VIM in less than 15 min with the sensitivity and specificity of 100% and 100%, respectively. The RESIST-5 O.O.K.N.V. detected KPC, NDM, OXA-48-like, and VIM with the sensitivity and specificity of 99.4 and 100%. The IMP K-SeT detected all of the IMP producers (6/6). For reference strains, the sensitivity and specificity of NG-test Carba 5, RESIST-5 O.O.K.N.V., and IMP K-SeT were all 100 and 100%, respectively.ConclusionAs efficient, rapid, and convenient diagnostic methods, NG-test Carba 5, RESIST-5 O.O.K.N.V., and IMP K-SeT could help to simplify the complex routine workflow for detecting carbapenemases. Rapid and accurate identification of carbapenemase is of significance for both epidemiological and infection control purposes.

Evaluation of the Performances of the Rapid Test RESIST-5 O.O.K.N.V Used for the Detection of Carbapenemases-Producing Enterobacterales

“…detected OXA-48 and NDM-like carbapenemases from 4 h subcultures on blood culture samples with a sensitivity of 100% [ 18 ]. They also agree with the results of the study by Han et al where the detection sensitivity of OXA-48 was 100%, however, the detection sensitivity of NDM was 97.1% [ 19 ]. Furthermore, studies carried out with the RESIST-4 O.K.N.V.…”

Detection of the Production of Klebsiella Pneumoniae Carbapenemase, New Delhi Metallo-Beta-Lactamase and Oxacillinase-48-Type Carbapenemases by Gram-Negative Bacilli in Resource-Limited Setting

“…In a study carried out in Turkey on isolates of K. pneumoniae, the results obtained by the RESIST-3 OKN K-SeT im- muno-chromatographic method were identical to those obtained by PCR targeting the carbapenemase genes bla KPC , bla NDM and/or bla OXA-48 with 100% concordance [25]. Han et al demonstrated the good performances of rapid immunochromatographic tests on clinical isolates, in comparison with molecular techniques (sensitivity greater than 99.4% according to the manufacturer and specificity of 100% for all the tests used in their study) [26].…”

Detection of Multidrug-Resistant Enterobacterales—From ESBLs to Carbapenemases

“…For carbapenemases, different tests are commercially available and have been extensively studied. The NG-Test CARBA-5 (NG biotech, Guipry, France) detects the most prevalent carbapenemases KPC, OXA-48-like, VIM, IMP, and NDM from bacterial colonies and directly from positive blood cultures with high sensitivity and specificity (84.2–99.2% and 95.3–100%, respectively), ( Table 1 ) [ 55 , 69 , 71 , 72 ]. The RESIST-5 O.O.K.N.V.…”

“…as well as its predecessors RESIST-4 and RESIST-3 (Coris BioConcept, Gembloux, Belgium) can be performed from bacterial colonies as well as directly from positive blood cultures. They detect and differentiate between OXA-48-like, OXA-163 (only RESIST-5), KPC, NDM, and VIM (only RESIST-4 and -5) with high sensitivity and specificity (84.2–100% and 100%, respectively) [ 55 , 67 , 68 , 69 , 147 ]. While the performance for KPC and OXA-48-like carbapenemases is excellent (sensitivity/specificity 100% in most studies), lower sensitivity has been reported for some Ambler class B β-lactamases especially in Proteus spp.…”