Evaluation of the ImmunoCardSTAT! Rotavirus Assay for Detection of Group A Rotavirus in Fecal Specimens

Dennehy, Penelope H.; Hartin, Michele; Nelson, Sara M.; Reising, Shirley F.

doi:10.1128/jcm.37.6.1977-1979.1999

Cited by 38 publications

(6 citation statements)

References 18 publications

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“…The LA technique has lower sensitivity than EIA [6,194]. ICG showed high sensitivity and results comparable to those achieved with EIA, and is rapid and technically very simple [195,196].…”

Section: Detection Of Gastroenteritis‐producing Virusesmentioning

confidence: 99%

Viruses causing gastroenteritis

Wilhelmi¹,

Román

Sánchez-Fauquier

2003

Clinical Microbiology and Infection

319

222

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Acute gastroenteritis is one of the most common diseases in humans worldwide. Viruses are recognized as important causes of this disease, particularly in children. Since the Norwalk virus was identified as a cause of gastroenteritis, the number of viral agents associated with diarrheal disease in humans has steadily increased. Rotavirus is the most common cause of severe diarrhea in children under 5 years of age. Astrovirus, calicivirus and enteric adenovirus are also important etiologic agents of acute gastroenteritis. Other viruses, such as toroviruses, coronaviruses, picobirnaviruses and pestiviruses, are increasingly being identified as causative agents of diarrhea. In recent years, the availability of diagnostic tests, mainly immunoassays or molecular biology techniques, has increased our understanding of this group of viruses. The future development of a safe and highly effective vaccine against rotavirus could prevent, at least, cases of severe diarrhea and reduce mortality from this disease.

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“…The LA technique has lower sensitivity than EIA [6,194]. ICG showed high sensitivity and results comparable to those achieved with EIA, and is rapid and technically very simple [195,196].…”

Section: Detection Of Gastroenteritis‐producing Virusesmentioning

confidence: 99%

Viruses causing gastroenteritis

Wilhelmi¹,

Román

Sánchez-Fauquier

2003

Clinical Microbiology and Infection

319

222

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“…Specific diagnosis of infection with Rotavirus is made by identification of the virus in the patient ' s stool by enzyme immunoassay several licensed test kit are used which are sensitive, specific and detect all serotype of Rotavirus [7,8,9,10]. These kits are also used to diagnosis infections of animals [10].…”

Section: Discussionmentioning

confidence: 99%

Performance of Enzyme Linked Immunosorbent Assay Versus Latex Agglutination Test in the Diagnosis of Acute Gastroenteritis by Rota Virus

Abood¹

2010

JNUS

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In this study we use two types of methods for detecting Rota virus in 91 stool specimens from children with acute gastroenteritis. The aim of this study to determine the performance (sensitivity & specificity) of latex agglutination (LAT) and enzyme linked immunos-orbentassay (ELISA) for evaluation children acute gastroenteritis by Rotavirus. Fecal samples were collected from ninety one children suffering from acute gastroentritis. their age ranged between(1 132) months. The highest sensitivity was(92.5%) obtained with LAT followed by ELISA (84.09%). while the highest specificity was(93.6%) obtained with ELISA followed by LAT(86.3%). the highest predictive positivity value was obtained with ELISA(92.5%) followed by LAT (84.09%). LAT is easy to performance and gave high sensitivity with accepted specificity therefore, could be applied successfully for routine diagnosis and Epidemiological study. But ELISA techniques allow quantitative estimation of Rotavirus antigens.

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“…, 2015, 1(1 HRV particles were detected by EM and/or IEM in 53 cases (41.7%) and by SPIEM in 5 additional cases; HRV antigens were demonstrated by indirect DAS ELISA in the same 53 cases, whereas 40 cases (31.4%) were positive for HRV isolation in cell cultures. Results showed that 64 patients representing 50.3% seroconverted by IPA and ELISA, including the entire cases [55] positive for rotavirus detection in stools and 6 additional cases. Thus, SPIEM appeared to be the most sensitive technique for detecting a few virus particles in stool specimens, but HRV serology is the most sensitive method for diagnosing HRV infections retrospectively, when paired sera are drawn at an appropriate time.…”

Section: Genes Reviewmentioning

confidence: 99%

Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

R.K¹,

Oke²,

Anjorin³

et al. 2015

Genes Review

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Rotavirus is the major etiology of acute gastroenteritis in infants and young children worldwide. It has been estimated that about 39% of childhood diarrhoea hospitalizations are caused by the virus and nearly half a million children die from the infections each year especially in developing countries on the Asian subcontinent, Africa, and Latin America where health care facilities are in short supply. Stool specimens collected from the first to fourth days of illness are optimal for detection of the infection. Diagnosis was originally performed using electron microscopy, which is still occasionally used in centers where it is available. Routine diagnosis is however, now performed by antigen detection on feces using commercially available, simple, rapid immunochromatographic dipstick style kits which have superseded the earlier latex agglutination and enzyme immunoassay methods whose usefulness in diagnosis is still relevant. Reverse transcription polymerase chain reaction of feces is also available in some reference and research centers, and is particularly useful for identification of outbreaks due to serogroups other than group A. Similarly viral culture and serology are also available but do not play much roles in diagnosis of acute disease. Rotavirus infection is indeed a public health problem and early, rapid, accurate and reliable diagnostic techniques are essential for effective patient management and infection control.

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Evaluation of the ImmunoCardSTAT! Rotavirus Assay for Detection of Group A Rotavirus in Fecal Specimens

Cited by 38 publications

References 18 publications

Viruses causing gastroenteritis

Viruses causing gastroenteritis

Performance of Enzyme Linked Immunosorbent Assay Versus Latex Agglutination Test in the Diagnosis of Acute Gastroenteritis by Rota Virus

Evaluation of Techniques for the Diagnosis of Rotavirus Gastroenteritis in Paediatric Patients

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