Evaluation of the HLA-F variability in Brazil by next generation sequencing

Castelli, Erick C.; Buttura, Renato V.; Paz, Michelle A.; Porto, Iane O. P.; Ramalho, Jaqueline; Souza, Andréia S.; Veiga‐Castelli, Luciana C.; Mendes-Junior, Celso Teixeira; Donadi, Eduardo Antônio

doi:10.1016/j.humimm.2015.07.125

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, > 2000 alleles for HLA class I are known in humans [8]. There are 2 classes of HLA and HLA class I in turn falls in two categories of classical ( HLA-A and B ) and non-classical ( HLA-C , G , E and F ) [4], [9], [10], [11], [12], [13]. The main role of HLA class I is that this biological molecule acts as an identifying card for all nuclear cells of body to be proposed for natural killer cells (CD56CD16 [14]) that different interactions between them results in different outcomes [15].…”

Section: Introductionmentioning

confidence: 99%

A genomic study on distribution of human leukocyte antigen ( HLA ) -A and HLA-B alleles in Lak population of Iran

2017

View full text Add to dashboard Cite

Anthropological studies based on the highly polymorphic gene, human leukocyte antigen (HLA), provide useful information for bone marrow donor registry, forensic medicine, disease association studies, as well as infertility treatment, designing peptide vaccines against tumors, and infectious or autoimmune diseases. The aim of this study was to determine HLA-A and HLA-B allele frequencies in 100 unrelated Lak/lᴂk/individuals from Lorestan province of Iran. Finally, we compared the results with that previously described in Iranian population. Commercial HLA-Type kits from BAG (Lich, Germany) company were used for determination of the HLA-A and HLA-B allele frequencies in genomic DNA, based on polymerase chain reaction with sequence specific primer (PCR-SSP) assay. The differences between the populations in distribution of HLA-A and HLA-B alleles were estimated by chi-squared test with Yate's correction. The most frequent HLA-A alleles were *24 (20%), *02 (18%), *03 (12%) and *11 (10%), and the most frequent HLA-B alleles were *35 (24%), *51 (16%), *18 (6%) and *38 (6%) in Lak population. HLA-A*66 (1%), *74(1%) and HLA-B*48 (1%), *55(1%) were the least observed frequencies in Lak population. Our results based on HLA-A and HLA-B allele frequencies showed that Lak population possesses the previously reported general features of Iranians but still with unique.

show abstract