Evaluation of the hill coefficient from scatchard and klotz plots

Sabouri, Ali Akbar; Moosavi‐Movahedi, Ali Akbar

doi:10.1016/0307-4412(94)90175-9

Cited by 35 publications

(5 citation statements)

References 2 publications

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“…The Hill coefficients were estimated as 0.55 and 0.52 for geraniol and citronellol , respectively. This indicates that the binding of geraniol and citronellol to hOR1A2 could be considered as negatively cooperative bindings 28 . Previous works show that for most GPCRs, the binding of one ligand to one binding site causes the structural change of neighboring binding sites, which may lead to a lower affinity for other ligands like our results 29 – 31 .…”

Section: Resultsmentioning

confidence: 94%

Human-like smelling of a rose scent using an olfactory receptor nanodisc-based bioelectronic nose

Lee

Yang

Kim

et al. 2018

Sci Rep

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We report a strategy for the human-like smelling of a rose scent utilizing olfactory receptor nanodisc (ND)-based bioelectronic nose devices. In this strategy, a floating electrode (FE)-based carbon nanotube (CNT) field effect transistor (FET) was functionalized with human olfactory receptor 1A2 (hOR1A2)-embedded NDs (hOR1A2NDs). The hOR1A2NDs responded to rose scent molecules specifically, which were monitored electrically using the underlying CNT-FET. This strategy allowed us to quantitatively assess the contents of geraniol and citronellol, the main components of a rose scent, as low as 1 fM and 10 fM, respectively. In addition, it enabled us to selectively discriminate a specific rose odorant from other odorants. Significantly, we also demonstrated that the responses of hOR1A2NDs to a rose scent could be strongly enhanced by enhancer materials like a human nose. Furthermore, the method provided a means to quantitatively evaluate rose scent components in real samples such as rose oil. Since our method allows one to quantitatively evaluate general rose scent ingredients just like a human nose, it could be a powerful strategy for versatile basic research and various applications such as fragrance development.

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Section: Resultsmentioning

confidence: 94%

Human-like smelling of a rose scent using an olfactory receptor nanodisc-based bioelectronic nose

Lee

Yang

Kim

et al. 2018

Sci Rep

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“…2 lower panel), were linear indicating no deviations from Clark's model and the single binding site n pointed out the relative high binding selectivity of the photochromes towards the biopolymer reactive sites. 12 It is worth noting that the reported values of n must be considered apparent for the following reasons: (i) the association constants are calculated by a single-site binding model, (ii) the observable physical quantity used, i.e. absorption, is proportional to the concentration (extensive variable) and not to the mole fraction (intensive variable e.g.…”

Section: Uv-vis Quantitative Data Analysismentioning

confidence: 99%

Effective control of the intrinsic DNA morphology by photosensitive polyamines

et al. 2017

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“…Positive cooperative binding results in sigmoidal MM and Klotz curves ( Figure 6A and B) and nonlinear Lineweaver-Burk (LB) plots bending upwards ( Figure 6C). [63] On the other hand, negative cooperative effect could give rise to biphasic primary curves ( Figure 6A) and nonlinear LB plots bending downwards [59,[64][65][66] (Figure 6 C). Data fitted to Equation (9) with a Hill coefficient (n) greater than or less than 1 are indicative of positive or negative cooperative effects, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Positive cooperative binding results in sigmoidal MM and Klotz curves (Figure 6 A & B) and non-linear Lineweaver-Burk (LB) plots bending upwards (Figure 6 C) 62 . On the other hand, negative cooperative effect could give rise to biphasic primary curves (Figure 6 A) and non-linear LB plots bending downwards 58,[63][64][65] (Figure 6 C).…”

Section: Introductionmentioning

confidence: 99%

Explicit Treatment of Non‐Michaelis‐Menten and Atypical Kinetics in Early Drug Discovery**

Srinivasan

2020

ChemMedChem

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Biological systems are highly regulated. They are also highly resistant to sudden perturbations enabling them to maintain the dynamic equilibrium essential to sustain life. This robustness is conferred by regulatory mechanisms that influence the activity of enzymes/proteins within their cellular context to adapt to changing environmental conditions. However, the initial rules governing the study of enzyme kinetics were mostly tested and implemented for cytosolic enzyme systems that were easy to isolate and/or recombinantly express. Moreover, these enzymes lacked complex regulatory modalities. Now, with academic labs and pharmaceutical companies turning their attention to more‐complex systems (for instance, multiprotein complexes, oligomeric assemblies, membrane proteins and post‐translationally modified proteins), the initial axioms defined by Michaelis‐Menten (MM) kinetics are rendered inadequate, and the development of a new kind of kinetic analysis to study these systems is required. This review strives to present an overview of enzyme kinetic mechanisms that are atypical and, oftentimes, do not conform to the classical MM kinetics. Further, it presents initial ideas on the design and analysis of experiments in early drug‐discovery for such systems, to enable effective screening and characterisation of small‐molecule inhibitors with desirable physiological outcomes.

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Evaluation of the hill coefficient from scatchard and klotz plots

Cited by 35 publications

References 2 publications

Human-like smelling of a rose scent using an olfactory receptor nanodisc-based bioelectronic nose

Human-like smelling of a rose scent using an olfactory receptor nanodisc-based bioelectronic nose

Effective control of the intrinsic DNA morphology by photosensitive polyamines

Explicit Treatment of Non‐Michaelis‐Menten and Atypical Kinetics in Early Drug Discovery**

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