Evaluation of the hepatoprotective, anti-inflammatory, antinociceptive and antiepileptic activities of Chrysanthemum trifurcatum

Salem, Gamal A.; Alamyel, Fathi B.; Abushaala, Faraj A.; Hussain, Md. Sarfaraj; Elnory, khloud A.; Abusheba, Hawa; Sahu, Ravi P.

doi:10.1016/j.biopha.2019.109123

Cited by 12 publications

(17 citation statements)

References 45 publications

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“…From the above scavenging activity, it was found that 400 μg/mL of MESAL having higher antioxidant activity as compared to ascorbic acid. The antioxidant activity of 400 μg/mL of MESAL may be due to the presence of ferulic acid in their extract [16][17][18] .…”

Section: Accuracy (Recovery)mentioning

confidence: 99%

“…Based on acute toxicity data, two different dosages 100, 200 and 400 mg/kg (p.o.) were selected for in-vivo antiinflammatory study [16][17] .…”

Section: Acute Toxicity Studies Of Mesalmentioning

confidence: 99%

“…In comparison, the anti-inflammatory effect of Diclofenac at 75 mg/kg was 0.767 at the same time. These results suggest that the MESAL at 100, 200, and 400 µg/ml exhibits the anti-inflammatory property in the acute phase of inflammation, but the antiinflammatory activity is more significant in dose 400 µg/ml, and mechanism of action may be associated with inhibition of the some of the inflammatory mediators like histamine, serotonin, bradykinins, and prostaglandins [17][18][19][20][21] .…”

Section: Acute Toxicity Studies Of Mesalmentioning

confidence: 99%

“…Different extract doses (0.5, 1, and 2 g / kg) were suspended and administered orally in 0.5 percent aqueous Tween 80. For indications of toxicity, e.g., autonomy, central nervous system, and behavioral modifications, the animals were continually noted for 12 h, and death was observed for 24 h [16][17] .…”

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confidence: 99%

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2021

IJPSR

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Now a day's interest towards natural and has been growing due to the unhealthy consequences of chemicals in the health industry; though, herbal substances possess several quality control and confirm pharmacological action issues. This present study was designed to determine the effects of Salix alba L. methanolic extract (MESAL) for its antioxidant and anti-inflammatory activity in rat models. Further to establish and validate a sensitive, fast and reproducible high performance thin layer chromatographic (HPTLC) method of two biomarker compounds Salicin and Ferulic acid from MESAL. The antiinflammatory activity was studied by the carrageenan-induced rat paw oedema method while DPPH free radical scavenging ability was utilized to determine the antioxidant activity. Additionally, the separation was performed by HPTLC with quantification of markers (Salicin and Ferulic acid). Among the different combinations of mobile phases used, the best separation was achieved in Toluene: Ethyl acetate: Methanol: Formic Acid (5:3:1:1v/v/v/v). The MESAL exhibited antioxidant activity with a maximal inhibitory concentration (IC 50 ) value of 400 µg/ml, and exerted anti-inflammatory activity, wherein 70 % protection was shown at 400 mg/ml. In contrast, HPTLC method gave compact spots of Salicin and Ferulic acid at R f 0.22 ± 0.02 and 0.68 ± 0.02, respectively. The MESAL displayed potent antioxidant and anti-inflammatory properties. Statistical analysis proves that the HPTLC method is repeatable and selective for the estimation of the said drugs, thus can be used for routine analysis and quality control of raw material of Salix alba L.

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Section: Accuracy (Recovery)mentioning

confidence: 99%

“…Based on acute toxicity data, two different dosages 100, 200 and 400 mg/kg (p.o.) were selected for in-vivo antiinflammatory study [16][17] .…”

Section: Acute Toxicity Studies Of Mesalmentioning

confidence: 99%

Section: Acute Toxicity Studies Of Mesalmentioning

confidence: 99%

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2021

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“…The active ingredients such as flavonoids, terpenoids, and organic acids in chrysanthemum have pharmacological effects on relieving fatigue, protecting liver, regulating immunity, etc. [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacology, Molecular Docking, and Molecular Dynamic-Based Investigation on the Mechanism of Compound Chrysanthemum in the Treatment of Asthenopia

Qiu

Zheng

Zhou

et al. 2022

Computational and Mathematical Methods in Medicine

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As a clinical empirical prescription for ophthalmology, compound chrysanthemum has been used gradually and has a good effect on eye fatigue. However, the detailed mechanisms of antiasthenopia have not been studied. In order to clarify the mechanisms of the compound chrysanthemum in the treatment of asthenopia, network pharmacology was combined with experimental study in this paper. A total of 593 genes and 39 active chemicals were identified, and both were considered to be essential to the advancement of asthenopia research. The results of the molecular docking analysis demonstrated a certain affinity between PRKACA, PRKCA, PRKCB, and their related compounds; molecular dynamic simulations assessed the stability of these receptors and ligands. The effects of compound chrysanthemum extract on ciliary muscle were studied in vitro and in vivo. By using the MTT assay, compound chrysanthemum extracts (50, 100, 200, 400, and 800 g·mL-1) showed no effect on the proliferation of rCSMCs for 24 and 48 hours. It raised nitric oxide and decreased Ca2+ in ciliary muscle cells isolated from the eyeballs of rats. Besides, compound chrysanthemum extract had a direct relaxing effect on the isolated gastric smooth muscle of rats by reducing the contractile tension. Furthermore, in vivo experiment results showed that, compared to the incandescent lamp-irradiated rats (model group), SD rats treated with compound chrysanthemum extracts (660 mg·kg-1 and 1320 mg·kg-1, orally) displayed considerably retracted pupils and increased NO content. It is also found that compound chrysanthemum extract can downregulate the mRNA expression of PKA and PKC in the calcium signaling pathway. Overall, our results suggested that compound chrysanthemum extract may lessen visual fatigue through multiple components, multiple targets, and multiple pathways.

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