Evaluation of the genotoxicity of cis-bis(3-aminoflavone)dichloroplatinum(II) in comparison with cis-DDP

Kośmider, Beata; Wyszyńska, Kalina; Janik-Spiechowicz, Ewa; Osiecka, Regina; Zyner, Elżbieta; Ochocki, Justyn; Ciesielska, Ewa; Wąsowicz, Wojciech

doi:10.1016/j.mrgentox.2003.11.006

Cited by 38 publications

(19 citation statements)

References 48 publications

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“…It is worth noticing that simultaneously, cis-Pt(II) complex of 3-aminoflavone was also less genotoxic and cytostatic in normal lymphocytes in comparison with cis-DDP. This new analogue induced apoptosis and necrosis in normal lymphocytes in a smaller degree than cis-DDP [41,42]. Moreover, our studies of cytotoxicity (MTT assay) indicated higher toxicity of cis-Pt(II) complex of 3-aminoflavone in lung cancer cell line A549 and simultaneously lower cytotoxic activity in normal lymphocytes.…”

Section: Discussionmentioning

confidence: 57%

Enhanced P53 and BAX gene expression and apoptosis in A549 cells by cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP

et al. 2005

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Lung cancer remains one of the most common causes of cancer-related death worldwide. Approximately 80% is histologically non-small cell lung carcinoma (NSCLC) and in about 70% of patients it is an unresectable type. Clinical studies indicated that application of platinum derivatives caused good results and combinations of platinum with other agents could improve median survivals. In view of the central problem of sufficient efficiency of drugs in chemotherapy, efforts have focused on the development of alternative platinum-based analogues that can be more effective in cancer treatment. cis-bis(3-aminoflavone)dichloroplatinum(II) (cis-Pt(II) complex of 3-aminoflavone) represents a novel class of platinum-based potential antitumour agents. In order to evaluate the degree of apoptosis, acridine orange/ethidium bromide and Hoechst 33258/propidum iodide double staining as well as RT-PCR (P53 and BAX expression evaluation) were used in lung cancer cell line A549 after treatment with this compound in comparison with cis-diamminedichloroplatinum(II) (cis-DDP). Apoptotic cells at early and late stages and also necrotic ones were observed after usage of cis-Pt(II) complex of 3-aminoflavone and the percentage of these cells outnumbered the values obtained after cis-DDP application. The former compound induced a higher percentage of P53 and BAX expression in A549 cells in comparison with the latter one. Results indicate the beneficial properties of cis-Pt(II) complex of 3-aminoflavone as a potential antitumor drug.

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Section: Discussionmentioning

confidence: 57%

Enhanced P53 and BAX gene expression and apoptosis in A549 cells by cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP

et al. 2005

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“…In the 1 H NMR spectra of the ligand, recorded in DMSO-d 6 , the signal of NH (1) was at 8.59 ppm, the signals of two diastereotopic CH2 groups in the 3.33-3.03 ppm range] and the N-NH2 protons resonated as a singlet at 4.74 ppm (atom numbering according to Figure 1).…”

Section: Resultsmentioning

confidence: 99%

Spectroscopic investigation, DFT calculations and cytotoxic activity of a 1-amino-1,3-dihydrospiro[imidazoline-4,2-indene]-2,5-dione and its platinum and palladium complexes

Buyukliev¹,

Cherneva²,

Konstantinov³

et al. 2017

Arkivoc

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“…We hypothesise that cisplatininduced sensitisation may occur through reactive oxygen species. Cisplatin is a potent genotoxic agent, inducing sister-chromatid exchanges, chromosomal aberrations and micronucleus formation in cultured human lymphocytes [17,22].…”

Section: Discussionmentioning

confidence: 99%

Sensitivity to cisplatin-induced mutations and elevated chromosomal aberrations in lymphocytes from sickle cell disease patients

et al. 2008

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Sickle cell disease (SCD) is an inherited disorder caused by a single nucleotide substitution in the beta-globin gene. The clinical heterogeneity observed in SCD patients has been attributed to environmental and genetic factors. The patients are subjected to increased oxidative stress, particularly during vaso-occlusive crises and acute chest pain. Another possible cause of oxidative stress in SCD is the high concentration of iron in the patients' plasma. The increase in oxidative stress could be a relevant risk factor for mutagenesis and carcinogenesis. Studies on the frequency of basal chromosomal aberrations in cultured lymphocytes from SCD patients have not been reported so far. In order to contribute to the understanding of the role of the different biomarkers and their relationship with the extremely variable clinical manifestation of SCD, we investigated the frequency of chromosome damage in peripheral lymphocytes from sickle cells patients and healthy controls. We found an increased frequency of chromosome damage and percentage of aberrant metaphases in these patients when compared with control subjects, even at basal values (p<0.05). In the cytogenetic sensitivity assay, the results showed that these patients presented a marked decrease in the mitotic index values compared with healthy controls. Cisplatin-induced chromosomal damage in lymphocytes from these patients was significantly higher than the frequency measured in healthy controls. The results obtained in the present study showed that more investigations are needed in order to elucidate the susceptibility to genomic instability of SCD patients.

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Evaluation of the genotoxicity of cis-bis(3-aminoflavone)dichloroplatinum(II) in comparison with cis-DDP

Cited by 38 publications

References 48 publications

Enhanced P53 and BAX gene expression and apoptosis in A549 cells by cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP

Enhanced P53 and BAX gene expression and apoptosis in A549 cells by cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP

Spectroscopic investigation, DFT calculations and cytotoxic activity of a 1-amino-1,3-dihydrospiro[imidazoline-4,2-indene]-2,5-dione and its platinum and palladium complexes

Sensitivity to cisplatin-induced mutations and elevated chromosomal aberrations in lymphocytes from sickle cell disease patients

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