Evaluation of the genotoxic potential of the Hypericum brasiliense (Guttiferae) extract in mammalian cell system in vivo

Espósito, Anderson Victorino; Pereira, Danielli Maria Vieira; Rocha, Leandro; Maistro, Edson Luis

doi:10.1590/s1415-47572005000100026

Cited by 9 publications

(6 citation statements)

References 9 publications

(10 reference statements)

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“…Frequency of aberrant metaphases in blank controls was within the usual range of 1.4–2.0% previously reported in the literature for Wistar male rats [ 25 , 26 , 27 ]. As expected, animals treated with CP showed a high frequency of abnormal metaphases (21.83 ± 3.25%) , which was comparable with the frequency of 16–21% determined by other authors in rats treated with a single 30–40 mg/kg dose of CP [ 25 , 27 , 28 ]. The most frequent CAs observed were chromatid breaks and chromatid exchanges (data not shown).…”

Section: Resultssupporting

confidence: 90%

Evaluation of the Biological Activity of Naturally Occurring 5,8-Dihydroxycoumarin

et al. 2013

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5,8-Dihydroxycoumarin (5,8-DHC) was isolated from aerial parts of sweet grass (Hierochloë odorata L.) and screened for antioxidant and genotoxic activities. A clear linear dependency of radical scavenging capacity in DPPH• and ABTS•+ assays was determined. 5,8-DHC was very efficient in retarding rapeseed oil oxidation (Oxipress test). TPC (total phenols content) and FRAP (the ability to reduce ferric ion to ferrous ion) assays revealed a somewhat lower antioxidant capacity of 5,8-DHC as compared with gallic acid. Genotoxic activity was tested using different genetic end-points: chromosome aberrations (CAs) and micronuclei (MN) in Wistar rat bone marrow in vivo, CAs and sister chromatid exchanges (SCEs) in human lymphocytes in vitro, and somatic mutations and recombination in Drosophila melanogaster wing cells in vivo. 5,8-DHC did not increase frequency of CAs in rat bone marrow cells, but induced a significant increase of MN. It was slightly mutagenic in the Drosophila melanogaster assay after 120 h of treatment, but not after 48 h of treatment. 5,8-DHC induced both CAs and SCEs in vitro in human lymphocytes in a clear dose-dependent manner. Thus, 5,8-DHC may be classified as weakly genotoxic both in vivo and in vitro.

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Section: Resultssupporting

confidence: 90%

Evaluation of the Biological Activity of Naturally Occurring 5,8-Dihydroxycoumarin

et al. 2013

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“…Tais resultados são comparáveis aos relatados por Espósito et al (2005) que avaliaram o efeito tóxico de Hypericum brasiliense.…”

Section: Resultsunclassified

Avaliação do efeito mutagênico do extrato hidroalcoólico bruto, por meio de bioensaios in vivo e prospecção fitoquímica de Cecropia glaziovii Sneth (embaúba), Cecropiaceae

Stange¹,

Gomes²,

Andrade³

et al. 2009

Rev. bras. farmacogn.

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RESUMO:O gênero Cecropia é conhecido popularmente por "embaúba" e apresenta várias espécies medicinais, sendo que estudos com a espécie C. glaziovii Sneth indicam que o extrato aquoso apresenta efeitos broncodilatador, anti-hipertensivo e antidepressivo, provavelmente, atribuídos às catequinas, procianidinas e flavonóides. O presente estudo objetivou avaliar os potenciais efeitos tóxico, citotóxicos, clastogênicos e aneugênicos do extrato bruto hidroalcoólico de folhas de C. glaziovii, por meio da dose letal média (DL 50 ), da análise de micronúcleo em células de medula óssea de roedores e do teste com Allium cepa e realizar a prospecção fitoquímica. Os resultados demonstraram que as concentrações testadas não causaram toxicidade aguda, nem apresentaram atividade clastogênica e aneugênica, sugerindo que o extrato de C. glaziovii não interferem na divisão celular. A prospecção fitoquímica indicou a presença de taninos, flavonóides, fenóis, antraquinonas, cumarinas, catequinas, proteínas, açúcares redutores, depsídeos/depsidonas e triterpenos. Mesmo assim, esses resultados não prescrevem o consumo terapêutico da espécie, sem continuidade da sua avaliação e do cumprimento de todas as etapas pré-clínicas e clínicas.Unitermos: Cecropia glaziovii, Cecropiaceae, mutagênese, micronúcleos, planta medicinal.ABSTRACT: "Evaluation of mutagenic effect of crude hydroalchoolic extract, using in vivo alive bioassays and phytochemical screening of Cecropia glaziovii Sneth (embaúba), Cecropiaceae". The genus Cecropia is as known popularly "embaúba" and presents several medical species. Studies with the species C. glaziovii Sneth indicates that the hydroalchoolic extracts presents bronchodilator, anti-hypertensive and antidepressive effects, probably due its contests catechins, procyanidins and flavonoids. The aim of this study was to assess the potential toxic, cytotoxic, clastogenic and aneugenic effects of the crude hydroalchoolic extract of leaves of C. glaziovii, by means of the determination of the median lethal dose (LD 50 ), the analysis of micronucleus in cells from bone marrow of rodents and through the Allium cepa assay, and also to performe the phytochemical screening. The results showed that at the concentrations tested no cause acute toxicity, as well as no clastogenic and aneugenic activity, was observed suggesting that the extract of C. glaziovii do not interfere in cell division. The phytochemical screening indicated the presence of tannins, flavonoids, phenols, anthraquinones, coumarins, catechins, proteins, reducing sugars, depsides, depsidons and triterpenes. Howerver, these results do not prescribe therapeutic consumption of the species, without keeping its evaluation and the achievement of all pre-clinical and clinical stages.

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“…For example, Sarimahmut et al 102 showed that H. adenotrichum extract possessed genotoxic effects on human lymphocytes demonstrated by different methods including comet assay. In contrast, another study showed that H. brasiliense extract did not exhibit a genotoxic effect in in vivo rat models 103 . In several studies, anti‐genotoxic effects of Hypericum spp extracts were found.…”

Section: Discussionmentioning

confidence: 91%

“…In contrast, another study showed that H. brasiliense extract did not exhibit a genotoxic effect in in vivo rat models. 103 In several studies, anti-genotoxic effects of Hypericum spp extracts were found.…”

Section: Genotoxicity Of H Alpestrementioning

confidence: 99%

Hypericum alpestre extract exhibits in vitro and in vivo anticancer properties by regulating the cellular antioxidant system and metabolic pathway of L‐arginine

Ginovyan,

Javrushyan,

Karapetyan

et al. 2024

Cell Biochemistry & Function

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Conventional treatment methods are not effective enough to fight the rapid increase in cancer cases. The interest is increasing in the investigation of herbal sources for the development of new anticancer therapeutics. This study aims to investigate the antitumor capacity of Hypericum alpestre (H. alpestre) extract in vitro and in vivo, either alone or in combination with the inhibitors of the l‐arginine/polyamine/nitric oxide (NO) pathway, and to characterize its active phytochemicals using advanced chromatographic techniques. Our previous reports suggest beneficial effects of the arginase inhibitor NG‐hydroxy‐nor‐ l‐arginine and NO inhibitor NG‐nitro‐Larginine methyl ester in the treatment of breast cancer via downregulation of polyamine and NO synthesis. Here, the antitumor properties of H. alpestre and its combinations were explored in vivo, in a rat model of mammary gland carcinogenesis induced by subcutaneous injection of 7,12‐dimethylbenz[a]anthracene. The study revealed strong antiradical activity of H. alpestre aerial part extract in chemical (DPPH/ABTS) tests. In the in vitro antioxidant activity test, the H. alpestre extract demonstrated pro‐oxidant characteristics in human colorectal (HT29) cells, which were contingent upon the hemostatic condition of the cells. The H. alpestre extract expressed a cytotoxic effect on HT29 and breast cancer (MCF‐7) cells measured by the MTT test. According to comet assay results, H. alpestre extract did not exhibit genotoxic activity nor possessed antigenotoxic properties in HT29 cells. Overall, 233 substances have been identified and annotated in H. alpestre extract using the LC‐Q‐Orbitrap HRMS system. In vivo experiments using rat breast cancer models revealed that the H. alpestre extract activated the antioxidant enzymes in the liver, brain, and tumors. H. alpestre combined with chemotherapeutic agents attenuated cancer‐like histological alterations and showed significant reductions in tumor blood vessel area. Thus, either alone or in combination with Nω‐OH‐nor‐ l‐arginine and Nω‐nitro‐ l‐arginine methyl ester, H. alpestre extract exhibits pro‐ and antioxidant, antiangiogenic, and cytotoxic effects.

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Evaluation of the genotoxic potential of the Hypericum brasiliense (Guttiferae) extract in mammalian cell system in vivo

Cited by 9 publications

References 9 publications

Evaluation of the Biological Activity of Naturally Occurring 5,8-Dihydroxycoumarin

Evaluation of the Biological Activity of Naturally Occurring 5,8-Dihydroxycoumarin

Avaliação do efeito mutagênico do extrato hidroalcoólico bruto, por meio de bioensaios in vivo e prospecção fitoquímica de Cecropia glaziovii Sneth (embaúba), Cecropiaceae

Hypericum alpestre extract exhibits in vitro and in vivo anticancer properties by regulating the cellular antioxidant system and metabolic pathway of L‐arginine

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