Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells

Abstract

Nano Ag has excellent antibacterial properties and is widely used in various antibacterial materials, such as antibacterial medicine and medical devices, food packaging materials and antibacterial textiles. Despite the many benefits of nano-Ag, more and more research indicates that it may have potential biotoxic effects. Studies have shown that people who ingest nanoparticles by mouth have the highest uptake in the intestinal tract, and that the colon area is the most vulnerable to damage and causes the disease. In this study, we examined the toxic effects of different concentrations of Ag-NPs on normal human colon cells (NCM460) and human colon cancer cells (HCT116). As the concentration of nanoparticles increased, the activity of the two colon cells decreased and intracellular reactive oxygen species (ROS) increased. RT-qPCR and Western-blot analyses showed that Ag NPs can promote the increase in P38 protein phosphorylation levels in two colon cells and promote the expression of P53 and Bax. The analysis also showed that Ag NPs can promote the down-regulation of Bcl-2, leading to an increased Bax/Bcl-2 ratio and activation of P21, further accelerating cell death. This study showed that a low concentration of nano Ag has no obvious toxic effect on colon cells, while nano Ag with concentrations higher than 15 μg/mL will cause oxidative damage to colon cells.

The Potential of Silver Nanoparticles for Antiviral and Antibacterial Applications: A Mechanism of Action

“…Moreover, the uses of AgNPs in the textile industry have gained quite a lot of attention due to the greater ion release and increasing catalytic activity as a result of the large surface area per mass of AgNP. The AgNPs are embedded into the fabric by two primary methods: (1) inserting the substrates with pre-formed nanoparticles and (2) inserting the substrates into a solution with silver salts and treating with reducing agents in order to convert silver salts into AgNPs [97]. A silver nanocoated fabric shows a homogeneous distribution of AgNPs and excellent antibacterial activity [98].…”

Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio

“…In the case of AgNP formulations evaluated in human colon tumor cells, most of them present their cytotoxic effect by ROS overproduction, which leads to apoptosis induction; however, limited information about coating agent or formulation stability is available [ 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ]. The results of this work agree with the general trend, all the formulations assessed promote mitochondrial ROS overproduction ( Figure 3 d) and trigger apoptosis ( Figure 3 b) as the major cell death pathway.…”

WT1-AS/IGF2BP2 Axis Is a Potential Diagnostic and Prognostic Biomarker for Lung Adenocarcinoma According to ceRNA Network Comprehensive Analysis Combined with Experiments

“…An equal volume of 2× sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer was added to the supernatant liquid, mixed well, and heated at 95 °C. The specific experimental details of Western blotting refer to previous research methods [ 27 ]. The GAPDH protein was used as an internal reference.…”