Evaluation of the fluorescein diacetate (FDA) vital dye viability test with hamster and bovine embryos

The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes

“…To assess their vitality, oocytes were incubated for 10 min at 37˚C in 2.5 µg/L fluorescein diacetate fluorochrome in PBS. Oocytes were washed in PBS before being observed with an epifluorescence microscope (using 450 -490 nm and 520 nm excitation and emission filters respectively) at 100× magnification (active estearases present in live oocytes hydrolyse the fluorochrome to render a green fluorescent compound) [22] [23].…”

The use of eosin B to assess the viability and developmental potential of rat embryos

“…However, it has been reported that exposure to fluorescein diacetate did not affect embryonic survival to day 17 of pregnancy for the blastocyst stage embryos of the mouse (Mohr and Trounson 1980) and exposure to neutral red and trypan blue did not affect development to term following transfer of frozen-thawed mouse blastocysts to pseudopregnant recipients (Thadani et al 1982). Others have reported that exposure of embryos to fluorescein diacetate prior to transfer did not affect the post-transfer viability of bovine blastocysts (Hoppe and Bavister 1984), 8-cell hamster embryos (Hoppe and Bavister 1984) or rabbit embryos collected 2 to 4 days after mating (Schilling et al 1979). …”

“…From these observations, it is concluded that multiple exposures of the rat embryo to micromolar concentrations of eosln B does not alter the developmental potential of the embryo. Comparable studies have not been performed for the other dye assays reported to be efficacious for the assessment of embryonic viability (Church and Raines 1980, Hoppe and Bavister 1984, Hutz et al 1985, Mohr and Trounson 1980, Schilling et al 1979). …”

“…Evaluation of the staining response of embryos while immersed in dye has been reported (Brock andRowson 1950, Hutz et al 1985). However, most methods for embryonic assessment using dyes require or recommend that the embryo is washed prior to evaluation of the staining response (Church and Raines 1980, Hoppe and Bavister 1984, Kardymowicz 1972, Mohr and Trounson 1980, Schilling et al 1979, Thadani et al 1982. Of those studies, only a few (Mohr andTrounson 1980, Thadani et al 1982) exposed the embryo to the dye in the same medium used to culture the embryo and then determined the staining response, after washing…”

“…Rhodamine 123 and ethidium bromide has been used to estimate viability of human spermatozoa (Evenson et al 1982 (Church and Raines 1980, Hoppe and Bavister 1984, Renard et al 1982, Schilling et al 1979, hamster (Hoppe andBavister 1984, Hutz et al 1985), mouse (Jackowski 1977, Mohr andTrounson 1980), rabbit (Schilling et al 1979), and squirrel monkey (Chan et al, 1982).…”