Evaluation of the establishment of native Mediterranean plant species suggested for landscape enhancement in archaeological sites of Greece

Papafotiou, Μ.; Bertsouklis, Κ.F.; Martini, Alessandro; Vlachou, G.; Akoumianaki‐Ioannidou, A.; Kanellou, E.; Kartsonas, E.D.

doi:10.17660/actahortic.2017.1189.35

Cited by 10 publications

(5 citation statements)

References 5 publications

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“…C. nepeta has potential use as an ornamental landscape plant or groundcover for urban and suburban parks, gardens, and green roofs (Caneva et al, 2013;Casalini et al, 2017;Dunnett et al, 2008), as well as in restoration projects of Mediterranean archae-ological sites (Papafotiou et al, 2017a), and in the creation of meadows with native plant species (Gilbert and Anderson, 1998;Maurer et al, 2000), particularly in soils with low fertility (Bretzel et al, 2009a;Vannucchi et al, 2015). It showed good levels of coverage, great survival, size, and flowering performance on green roofs (Benvenuti and Bacci, 2010;Dunnett et al, 2008), as well as self-sowing and acceptable percentage of emergence (Benvenuti, 2014;Casalini, et al, 2017;Nagase et al, 2013).…”

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confidence: 99%

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Vlachou¹,

Papafotiou²,

Bertsouklis³

2019

horts

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Seed ecophysiology and micropropagation of Clinopodium nepeta, an aromatic Mediterranean plant with pharmaceutical and horticultural uses was investigated. The optimum germination temperature of seeds stored at room temperature for 0, 6, or 12 months was 15 to 20 °C (100% germination completed in 10 to14 days) and cardinal temperatures were defined at 10 and 30 °C (80% to 82% and 62% to 76% germination, respectively). Six or 12 months of storage did not seem to affect germination, although 12-month-old seeds germinated at higher percentage and completed germination earlier at 15 °C than at 20 °C. Concerning micropropagation, shoot multiplication at subcultures of both adult plant- and seedling-origin nodal explants was tested on Murashige and Skoog (MS) medium supplemented with various cytokinin types, i.e., zeatin (ZEA), 6-benzyladenine (BA), kinetin (KIN), and 6-γ-γ-(dimethylallylamino)-purine (2IP), at various concentrations from 0.0 to 8.0 mg·L−1. Both explant types presented a rather similar response during in vitro culture. Increasing concentration of all cytokinin types resulted in an increase in shoot number per responding explant (1.1–5.3) and in most cases a decrease in shoot length (0.6–3.4 cm). Increasing cytokinin concentration induced hyperhydricity to a number of shoots (0.1–6.5) per explant, mostly when ZEA and BA were used. Supplementing the MS medium with 8.0 mg·L−1 BA combined with 0.1 mg·L−1 1-naphthaleneacetic acid (NAA) led to almost elimination of hyperhydricity and very satisfactory shoot production (80%/88% explant response and 6.5/7.5 shoot number per responding explant for seedling- / adult-origin explants, respectively). Alternatively, increasing the agar concentration to 12.0 g·L−1 and supplementing the medium with 8.0 mg·L−1 BA only, resulted in the same effect on eliminating hyperhydricity, such as the addition of NAA, and in the best shoot multiplication response achieved in this study (100% explant response, 9.4/9.9 shoots per explant for seedling-/adult-origin explants, respectively). Microshoots rooted abundantly (92% to 100%) on half-strength MS medium, either Hf or supplemented with 0.5 mg·L−1 to 4.0 mg·L−1 indole-3-butyric acid (IBA). The addition of IBA to the rooting medium, regardless of its concentration, affected only the root length by increasing it 2- to 3-fold. Microshoot clusters produced on multiplication media rooted at 96% when cultured on Hf half-strength MS medium. Rooted microshoots and shoot clusters survived at 80% to 100%, respectively, after ex vitro acclimatization in peat:perlite 1:1 (v/v).

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confidence: 99%

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Vlachou¹,

Papafotiou²,

Bertsouklis³

2019

horts

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“…At the in vitro establishment stage, A. hermanniae explants from seedlings (juvenile explants) responded at higher percentages to produce shoots than explants from adult wild plants (adult explants). In MS medium with 1.0 mg L −1 BA, explants from seedlings produced significantly more shoots (14) compared to explants from adult native plants (6), verifying work on A. cytisoides [26]. In that work, the response of seedling-origin explants was also superior, although the number of shoots produced was low (5) in MS medium with 0.5 mg L −1 BA, and even fewer were the number of shoots (2) produced from seedling-origin explants of A. barba-jovis in the same medium as that of the present study [27,28].…”

Section: In Vitro Clonal Propagationmentioning

confidence: 99%

“…Due to the plant's appearance, as well as its tolerance in barren soils, xerothermic conditions, winds, and strong temperature changes, it is recommended to be used as an ornamental plant in sustainable urban and peri-urban green spaces, in rock gardens, xeriscaping, costal planting and degraded soil areas, as well as in archaeological sites of the Mediterranean region [6]. Its property of attracting pollinators and its limited needs for irrigation serves the current trend for sustainable landscaping.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Seed and Clonal Propagation of the Mediterranean Bee Friendly Plant Anthyllis hermanniae L.

Martini

Papafotiou

2023

Sustainability

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Anthyllis hermanniae L. (Fabaceae) is a perennial Mediterranean shrub with the potential to be used as a bee-friendly ornamental plant in arid and semi-arid regions, valued for its tolerance of barren soils, winds, and strong temperature changes. With the aim of facilitating the introduction of the species into the horticulture industry, its in vitro seed and clonal propagation was investigated for the first time, to our knowledge. Seeds stored in the dark at room temperature for 4, 7, 9, 12, and 18 months germinated at percentages higher than 80% after scarification, when incubated in vitro in solid half-strength Murashige and Skoog (MS) medium at temperatures from 10 to 25 °C, while photoperiod (continuous darkness or 16 h light period/8 h dark) during incubation did not affect germination. Explants excised from in vitro grown seedlings established at higher percentages compared to explants from adult native plants, more efficiently in MS medium with 1.0 mg L−1 6 N benzyladenine (BA). During subcultures in the same medium, juvenile explants formed more and longer shoots than adult ones. Almost all adult explants formed shoots when subcultured in MS medium with 0.0 to 4.0 mg L−1 BA, zeatin, kinetin or 6-(γ,γ-dimethylallylamino)purine (2iP). ΒΑ at 0.5 to 2.0 mg L−1 induced many more shoots (17–21) per explant and much higher multiplication indices compared to all other cytokinins, while longer shoots were produced in a medium without hormones or with 0.5–1.0 mg L−1 2iP. Microshoots cultured in half-strength MS medium with 0.0–4.0 mg L−1 indole-3-butyric acid rooted at highest percentage (around 70%) in the medium containing 4.0 mg L−1 IBA, while microshoots of juvenile origin developed more and longer roots compared to adult ones. Micropropagated plantlets were successfully acclimatized ex vitro (>97%), regardless of their origin. The efficient micropropagation of A. hermaniae will facilitate its sustainable exploitation as a bee-friendly landscape plant, a forage plant for honeybees in Mediterranean areas, and a medicinal plant.

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“…The findings will be useful for both the enhancement of natural values and the protection of monuments on the site, thus ensuring a balance between the need to preserve natural and cultural values, which is an aspect that should be better considered in the management plan of archeological sites [5,10,24,39,49,50].…”

Section: Introductionmentioning

confidence: 99%

Vegetation Analysis in the Archaeological Area of Pasargadae WHS (Iran) Enhancing the Naturalistic Value of the Site within the Occurring Environmental Changes

Zangari,

Hosseini,

Caneva

2024

Sustainability

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This study provides pioneering research on the vegetation of archaeological areas in Iran to enhance its naturalistic and bioindication values by selecting the Pasargadae World Heritage Site (WHS). Vegetation surveys were carried out in different homogeneous habitats, analyzing the plant communities through statistical elaboration, syntaxonomic role, mapping, and enhancement of plants with conservation interest. In an ecological approach, the study included an analysis of the recent climate changes and human interventions influencing the water resources. Results revealed seven main vegetation types reflecting ecological gradients shaped by environmental, edaphic, and anthropogenic factors. The syntaxonomic analysis showed a primary subdivision in semi-natural grasslands and synanthropic vegetation. Several key species were identified as bioindicators of multiple factors, such as: Launaea acanthodes, Stipa barbata, Alhagi maurorum, Bellevalia saviczii, Glycyrrhiza glabra, Convolvulus arvensis, and Hordeum murinum. The vegetation map showed how the hilly grassland communities hosted the highest number of species with conservation interest and their need to be better protected. Bio-climatic data, such as the construction of dams and the exploitation for irrigation purposes, pointed to the increasing xeric conditions, which make urging conservation efforts for the site’s historical and naturalistic values. The study underscores the importance of preserving places with high plant diversity for effective site management, and enhances the intricate relationship between vegetation and natural features in the occurring environmental changes.

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Evaluation of the establishment of native Mediterranean plant species suggested for landscape enhancement in archaeological sites of Greece

Cited by 10 publications

References 5 publications

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

In Vitro Seed and Clonal Propagation of the Mediterranean Bee Friendly Plant Anthyllis hermanniae L.

Vegetation Analysis in the Archaeological Area of Pasargadae WHS (Iran) Enhancing the Naturalistic Value of the Site within the Occurring Environmental Changes

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