Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus orientalis Leaf Infusion Against Ethanol Toxicity in Rats

Abstract

The aim of this study was to evaluate the protective effects of the leaf infusion of chinar (Platanus orientalis L.) on erythrocyte fragility, haematological parameters and antioxidant status against ethanol-induced oxidative stress in rats. Thirty male rats were divided into five groups: Control, Ethanol, Ethanol+Silymarin (10 mg kg -1 ), Ethanol+PO-20 mg mL -1 infusion, and Ethanol+PO-60 mg mL -1 infusion. According to the results, in the Ethanol group, erythrocyte counts, red cells distribution, plateletcrit, platelet and lymphocyte levels significantly decreased compared to the Control group, while PO-60 dose-fed group showed a significant increase in haematocrit and haemoglobin values compared to the Ethanol group. There were significant changes in erythrocyte fragility of Ethanol and Ethanol-treatment groups at different NaCl concentrations of 0.3 and 0.6 according to Control group. It was observed that PO Leaf infusion reduced the hemolysis caused by ethanol at a concentration of 0.3% NaCl, thus reducing the values to the control values. In addition, PO leaf infusion caused a significant increase in total antioxidant status against ethanol toxicity and a significant decrease in total oxidative status and oxidative stress index. It was concluded that PO leaf infusion may have antihematotoxic effect, reducing erythrocyte fragility and increase antioxidant capacity against ethanol toxicity.