Evaluation of the Efficacy of Granulocytic Colony-Stimulating Factor for the Treatment of Experimental Myocardial Destruction in Mice

Abstract

We studied the effects of recombinant granulocytic CSF on heart remodeling in BALB/c mice after cryodestruction. Administration of granulocytic CSF was started 1 day after cryodestruction (subcutaneously, 10 μg/kg/day, for 4 days). As early as after the first injection, leukocytosis in the peripheral blood started to develop, leukocyte count peaked on days 4-6 and returned to normal on day 14. Treatment with granulocytic CSF significantly increased the content of progenitor cells in the bone marrow and led to rapid development of the inflammatory reaction and myocardium infiltration with mononuclear cells. Injections of granulocytic CSF did not reduce scar area, but provided significantly less pronounced heart hypertrophy, which attests to its better functional properties. By day 30 after cryodestruction, control animals and animals receiving granulocytic CSF exhibited similar morphological picture at the site of damage. Thus, our regimen of granulocytic CSF administration produced a mobilizing effect on bone marrow progenitor cells and postinfarction heart remodeling. Direct effects of granulocytic CSF on the heart have to be established for its use in the treatment of myocardial infarction.

Radiofrequency Ablation as a Possible Method for Preparing Pathologically Altered Myocardium for Intramyocardial Cell Transplantation

“…MSC were obtained using BM mononuclear fraction from rat femoral bones; they were cultured for 14 days in a sterile CO 2 -incubator at 37 o C, 100% humidity, and 5% CO 2 [5,9].…”

“…Experimental group 1 comprised animals receiving transplantation of MSC after radiofrequency destruction [4,5]. Animals of group 2 (injection of an equivalent volume of culture medium after radiofrequency ablation) and group 3 (MSC transplantation without radiofrequency ablation) served as the control.…”