Evaluation of the efficacy of an autogenous <i>Escherichia coli</i> vaccine in broiler breeders

Li, Lili; Thøfner, Ida; Christensen, Jens Peter; Ronco, Troels; Pedersen, Karl; Olsen, Rikke Heidemann

doi:10.1080/03079457.2016.1267857

Cited by 34 publications

(25 citation statements)

References 19 publications

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“…Passive immunity by maternal derived antibodies protected the offspring of vaccinated breeders during the first two weeks of life (Rosenberger et al, 1985;Heller et al, 1990). In contrast, Li et al (2017) did not find transfer of E. coli antibodies from vaccinated parent stock to their progeny. In any case, maternal antibodies do not protect broilers during the second half of the grow-out period.…”

Section: Discussionmentioning

confidence: 78%

“…Literature regarding vaccination for the protection of laying hens against colibacillosis is very scare; to date only two manuscripts on this subject have been published (Gregersen et al, 2010;Li et al, 2017). Gregersen and others performed a field study in broiler breeders using a subunit vaccine (E. coli fimbrial antigen F11 and E. coli flagellar toxin in a water-in-oil emulsion).…”

Section: Discussionmentioning

confidence: 99%

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The efficacy of inactivated Escherichia coli autogenous vaccines against the E. coli peritonitis syndrome in layers

Landman

Eck

2017

Avian Pathology

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Autogenous Escherichia coli vaccines to prevent the E. coli peritonitis syndrome (EPS) in laying hens are often used in the field, although their effectiveness has not been demonstrated yet. Therefore, in this study, which consisted of two experiments, their efficacy was assessed. In the first experiment, the EPS-inducing ability of three E. coli isolates originating from bone marrow of hens that died due to EPS and with different Pulsed-Field Gel Electrophoresis patterns, was examined by intravenous inoculation of the isolates in 17-week-old brown layers. Based on the results one isolate was chosen for the preparation of the vaccines and for homologous challenge and another one for heterologous challenge performed in the second experiment. In the named experiment, groups of laying hens which had been vaccinated intramuscularly at 14 and 18 weeks of age with inactivated vaccine either formulated as aqueous suspension or as water-in-oil emulsion were homologously or heterologously challenged per aerosol at 30 weeks of age. The vaccines contained ≥10 formaldehyde-inactivated colony-forming units (cfu) of E. coli per hen dose in 0.5 ml. The estimated E. coli challenge dose uptake ranged from 10 to 10 cfu per hen. Groups consisted of 18 hens each and were housed in separate isolators from 27 weeks of age. Control groups were included in this experiment, which was ended eight days after challenge. Vaccinations had no effect on body growth and both vaccine types induced (almost) complete protection against homologous challenge, while protection against heterologous challenge was inconclusive.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

The efficacy of inactivated Escherichia coli autogenous vaccines against the E. coli peritonitis syndrome in layers

Landman

Eck

2017

Avian Pathology

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“…Vaez Zadah et al [ 47 ] reported that a booster dose of an O2 outer membrane protein (OMP)-based vaccine induces a significant increase in specific antibody titers. In contrast, Li et al [ 38 ] reported the limited clinical efficacy of an O78 autogenous vaccine even though they measured a significant rise in antibody titers but used different vaccination (subcutaneous) and inoculation (intra-oviduct) routes in broiler breeders. Most publications, however, report reduced mortality and macroscopic lesions as the common outcome of E. coli O78 vaccination, regardless of the vaccine used and experimental inoculation route [ 27 , 28 , 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…By nature, each autogenous vaccine is different, and reports on the impact of their usage are sporadic. As far as colibacillosis in layers/breeders is concerned, two reports have been published, with a negative [ 38 ] and a positive outcome [ 37 ]. However, to the authors’ knowledge, no publication has reported so far on the use of autogenous vaccines in a colibacillosis vaccination program, together with a commercial vaccine, evaluated by intratracheal challenge.…”

Section: Introductionmentioning

confidence: 99%

An Assessment of the Level of Protection Against Colibacillosis Conferred by Several Autogenous and/or Commercial Vaccination Programs in Conventional Pullets upon Experimental Challenge

Koutsianos

Gantelet

Franzo

et al. 2020

Veterinary Sciences

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The prevention of avian colibacillosis has historically been investigated through vaccination, with variable outcomes. Commercial live (attenuated) and inactivated vaccines are reported to have limited efficacy in the context of heterologous challenge. Autogenous vaccination, using field isolates, is widely used, but scarcely documented. Different vaccination programs, including a live commercial vaccine and/or an inactivated autogenous vaccine, were compared for three different avian pathogenic Escherichia coli (APEC) strain (serotypes O78, O18 and O111) challenges. On the pullet farm, four groups of conventional pullets received different vaccination protocols. Group A was kept unvaccinated (control group). Group B was vaccinated three times with a live commercial O78 E. coli vaccine (at one day old, 59 and 110 days of age). Group C was immunized twice (at 79 and 110 days) with a three-valence autogenous vaccine (O78, O18 and O111). Group D was vaccinated first with the commercial vaccine (at one day old and 59 days), then with the autogenous vaccine (110 days). Birds were transferred to the experimental facility at 121 days of age and were challenged 10 days later. In each group, 20 birds were challenged with one of the three APEC strains (O78, O18, O111); in total, 80 birds were challenged by the same strains (20 per group). The recorded outcomes were: mortality rate, macroscopic lesion score in target organs and the bacterial recovery of the challenge strain from bone marrow and pooled organs. When challenged with O78 or O111 strains, birds from groups C and D proved to be significantly better protected, in terms of lesion scoring and bacteriological isolation, than those of groups A and B. With the O18 challenge, only birds of group D presented a statistically significant reduction of their lesion score. To the authors’ knowledge, this is the first report on the efficacy of an immunization program in poultry that combines commercial and autogenous vaccines.

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“…Autogenous vaccines have been used in terrestrial agriculture including cattle, swine and poultry where they have successfully reduced antibiotic use (Arsenakis et al, 2018;Baums et al, 2010;Li et al, 2017;Reeves et al, 2013). For instance, exudative epidermitis caused by Staphylococcus hyicus is a common skin disease in pigs and autogenous vaccine against S. hyicus reduce morbidity and mortality rate while the amount of metaphylactic antimicrobial treatment has been reduced (Arsenakis et al, 2018).…”

Section: Chapter 5: General Discussionmentioning

confidence: 99%

A pan genome reverse vaccinology approach to prevent Streptococcus agalactiae infection in farmed tilapia

Kawasaki¹

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Streptococcus agalactiae (Group B Streptococcus; GBS) is a Gram-positive pathogenic bacterium that causes disease in a wide range of animals including fish. Outbreaks of GBS in farmed fish have substantial economic impacts on the aquaculture industry, especially in tilapia production, a staple food product in tropical and subtropical low-and middle-income countries and the second most widely farmed fish after carp. Although vaccination can effectively prevent disease caused by GBS in fish it often fails after relatively short periods of use. Capsular polysaccharide (CPS) is the major protective antigen in GBS vaccines and a critical virulence factor. There are currently ten CPS serotypes of GBS and, whilst vaccination against one serotype confers protection in fish, it is not cross-protective against other serotypes. In addition, under the strong selective pressure of vaccination-driven adaptive immunity, evolution of novel serotypes can occur. To improve the serotype crossprotective efficacy of GBS vaccines for aquaculture, I employed a pan-genome reverse vaccinology approach to design candidate serotype-independent protective vaccines based on conserved proteins in fish-pathogenic GBS serotypes. Reverse vaccinology exploits recent advances in genomics to predict antigens in the genome sequence. Reverse vaccinology combined with pan-genome can identify the antigens that are conserved across a diversity of strains of a pathogen.To identify potential vaccine candidate antigens, a pan-genome was constructed for GBS comprising 82 genomes including representative isolates from wild marine fish and stingrays, farm and companion animals and from human clinical cases in Australia, along with isolates from farmed tilapia in Honduras and representative genomes from all current serotypes available via the NCBI Genbank database. Core genome single nucleotide polymorphism 3 (SNP) analysis indicated dissemination of the aquatic host-adapted ST-261 lineage in wild Australian fish from an ancestral tilapia strain, which is congruent with several introductions of tilapia into northern Australia from Israel and Malaysia during the 1970s and 1980s.Aquatic serotype Ib GBS strains have lost many virulence factors during adaptation, but six adhesins, named ST-261 adhesins, were well-conserved across the aquatic isolates and might be critical for virulence in fish and good targets for vaccine development. Three of six ST-261 adhesins were also conserved across all strains including terrestrial isolates and were chosen as vaccine candidates. Expressions of ST-261 adhesin genes were inversely co-regulated with CPS expression dependent upon carbon source and metal ions in the medium. However, gene transcription was not reflective of surface expression as serum antibodies derived in tilapia against recombinant adhesins did not detect similar variation in the level of ST-261 adhesins in whole cell ELISA or Western blot. This may be due to posttranscriptional processing causing a disconnect between gene and protein expression.To evaluate th...

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Evaluation of the efficacy of an autogenous Escherichia coli vaccine in broiler breeders

Cited by 34 publications

References 19 publications

The efficacy of inactivated Escherichia coli autogenous vaccines against the E. coli peritonitis syndrome in layers

The efficacy of inactivated Escherichia coli autogenous vaccines against the E. coli peritonitis syndrome in layers

An Assessment of the Level of Protection Against Colibacillosis Conferred by Several Autogenous and/or Commercial Vaccination Programs in Conventional Pullets upon Experimental Challenge

A pan genome reverse vaccinology approach to prevent Streptococcus agalactiae infection in farmed tilapia

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