Evaluation of the Efficacy and Safety of a Marine-Derived Bacillus Strain for Use as an In-Feed Probiotic for Newly Weaned Pigs

Prieto, Maria Luz; O’Sullivan, L.; Tan, Shiau Pin; McLoughlin, Peter; Hughes, Helen; O'Donovan, Orla; Rea, Mary C.; Kent, Robert M.; Cassidy, Joseph P.; Gardiner, Gillian E.; Lawlor, Peadar G.

doi:10.1371/journal.pone.0088599

Cited by 35 publications

(39 citation statements)

References 38 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our study, the addition of probiotics resulted in an increase in the number of goblet cells in E2 + EM group, which received the probiotic (Figure 2). This confirms the study of Prieto et al (2014) using Bacillus pumilus, who after 22 days of supplementation of weaned piglets showed a higher number of goblet cells/1 villi (p < .01) compared to the control group (13.97 vs. 9.67). However, other authors did not show the effect of Enterococcus faecium on increasing the number of goblet cells in the jejunum of fattening pigs (Giannenas et al, 2016).…”

Section: Ta B L E 6 Porcine Jejunal Morphologysupporting

confidence: 88%

Influence of the effective microorganisms (EM) on performance, intestinal morphology and gene expression in the jejunal mucosa of pigs fed different diets

Reszka

Dunisławska

Sławińska

et al. 2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

The aim of the study was to determine the influence of the effective microorganisms (EM) on performance parameters, intestinal morphology and gene expression in the jejunal mucosa in pigs under different feeding regimes. The study group comprised of 150 piglets divided into three feeding groups: C, E1 and E2. Feeding groups included: C—standard fodder, blend with a full share of post‐extracted soy meal, E1—in the phase I of fattening: pea and lupin/soybean 50/50%; in the phase II of fattening: pea and lupin/soybean 75/25%, and E2—in the phase I of fattening: pea and lupin/soybean 50/50%; in the phase II of fattening: pea and lupin 100%. The experimental factor was addition of a probiotic EM Carbon Bokashi to the diets (C + EM, E1 + EM and E2 + EM). After slaughter, histological evaluation and gene expression analysis were performed. The highest intestinal villi were reported in E2 + EM. A higher intestinal absorption area was demonstrated in groups C + EM and E2 + EM. An interaction between feeding and EM Bokashi supplementation was found in villus surface area crypt depth, villus height/crypt depth and number of goblet cells. Mucosa thickness and number of goblet cells was the largest in E2 + EM. Gene expression of FABP4 increased in E1, and GLUT2 decreased in E2. Gene expression of IL10 and FABP4 increased in E2 + EM. The results indicate that the E2 diet is more optimal for EM Bokashi supplementation, because in this group, EM positively influenced the morphological characteristics of the porcine jejunum and caused an increase in the expression of genes related to the metabolism and functioning of the gastrointestinal tract.

show abstract

Section: Ta B L E 6 Porcine Jejunal Morphologysupporting

confidence: 88%

Influence of the effective microorganisms (EM) on performance, intestinal morphology and gene expression in the jejunal mucosa of pigs fed different diets

Reszka

Dunisławska

Sławińska

et al. 2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

show abstract

“…A 1.5-ml volume of the resultant supernatant and 1.5 ml internal standard were mixed gently and filtered through a 0.45-mpore-size Whatman filter (VWR International Ltd., Dublin, Ireland) into a labeled 8-mm amber GC vial (Antech Solutions Ltd., Waterford, Ireland). Extracts were stored at Ϫ80°C until analysis by gas chromatography, as previously described (25,69).…”

Section: Figmentioning

confidence: 99%

“…In particular, the enrichment of specific microbes, supported by beneficial functionality, could pinpoint prospective microbial biomarkers for FE within the porcine intestinal microbiota. Optimization of the microbiota could then potentially be achieved through the use of these specific bacterial taxa as probiotics or, alternatively, by increasing their abundance via the use of prebiotics or other dietary supplements (10,24,25) or by fecal microbiota transplantation (26).…”

mentioning

confidence: 99%

Exploring a Possible Link between the Intestinal Microbiota and Feed Efficiency in Pigs

McCormack

Curião

Buzoianu

et al. 2017

Appl Environ Microbiol

Self Cite

260

208

View full text Add to dashboard Cite

Feed efficiency (FE) is critical in pig production for both economic and environmental reasons. As the intestinal microbiota plays an important role in energy harvest, it is likely to influence FE. Therefore, our aim was to characterize the intestinal microbiota of pigs ranked as low, medium, and high residual feed intake ([RFI] a metric for FE), where genetic, nutritional, and management effects were minimized, to explore a possible link between the intestinal microbiota and FE. Eightyone pigs were ranked according to RFI between weaning and day 126 postweaning, and 32 were selected as the extremes in RFI (12 low, 10 medium, and 10 high). Intestinal microbiota diversity, composition, and predicted functionality were assessed by 16S rRNA gene sequencing. Although no differences in microbial diversity were found, some RFI-associated compositional differences were revealed, principally among members of Firmicutes, predominantly in feces at slaughter (albeit mainly for low-abundance taxa). In particular, microbes associated with a leaner and healthier host (e.g., Christensenellaceae, Oscillibacter, and Cellulosilyticum) were enriched in low RFI (more feed-efficient) pigs. Differences were also observed in the ileum of low RFI pigs; most notably, Nocardiaceae (Rhodococcus) were less abundant. Predictive functional analysis suggested improved metabolic capabilities in these animals, especially within the ileal microbiota. Higher ileal isobutyric acid concentrations were also found in low RFI pigs. Overall, the differences observed within the intestinal microbiota of low RFI pigs compared with that of their high RFI counterparts, albeit relatively subtle, suggest a possible link between the intestinal microbiota and FE in pigs.IMPORTANCE This study is one of the first to show that differences in intestinal microbiota composition, albeit subtle, may partly explain improved feed efficiency (FE) in low residual feed intake (RFI) pigs. One of the main findings is that, although microbial diversity did not differ among animals of varying FE, specific intestinal microbes could potentially be linked with porcine FE. However, as the factors impacting FE are still not fully understood, intestinal microbiota composition may not be a major factor determining differences in FE. Nonetheless, this work has provided a potential set of microbial biomarkers for FE in pigs. Although culturability could be a limiting factor and intervention studies are required, these taxa could potentially be targeted in the future to manipulate the intestinal microbiome so as to improve

show abstract

“…Enterococcus faecalis can improve the efficiency of feed utilization and can balance the microbial environment in weaned piglets [11]. Bacillus pumilus spore suspension can lower the incidence of diarrhea and improve growth performance after weaning [12]. Lactobacillus reuteri can also promote growth, reduce diarrhea and regulate the immune system of pigs [8].…”

Section: Introductionmentioning

confidence: 99%

Growth Performance and Post-Weaning Diarrhea in Piglets Fed a Diet Supplemented with Probiotic Complexes

Lu¹,

Zhang²,

Zhao³

et al. 2018

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

Weaning stress can affect the growth performance and intestinal health of piglets. Dietary alternatives to antibiotics, such as dietary probiotics, especially those containing multiple microbial species, are a preventive strategy for effectively controlling post-weaning diarrhea. In this study, we investigated forty-eight crossbred piglets in three treatment groups for 21 days: the control and experimental groups were supplemented with Enterococcus faecium DSM 7134, Bacillus subtilis AS1.836 plus Saccharomyces cerevisiae ATCC 28338 (EBS) or Lactobacillus paracasei L9 CGMCC No. 9800 (EBL). On day 21, weaned piglets supplemented with two kinds of probiotic complexes showed increased growth performance and significantly reduced postweaning diarrhea (p < 0.05). The EBS treatment increased acetic acid and propionic acid in the feces (p < 0.05), and the EBL treatment increased fecal acetic acid, propionic acid, butyrate and valerate (p < 0.05). Moreover, the fecal microbiota of the piglets changed markedly in EBL treatment. The addition of EBS and EBL may have similar effects on the prevention of diarrhea by improving the intestinal morphology and regulating the microbiota during the weaning period.

show abstract

Evaluation of the Efficacy and Safety of a Marine-Derived Bacillus Strain for Use as an In-Feed Probiotic for Newly Weaned Pigs

Cited by 35 publications

References 38 publications

Influence of the effective microorganisms (EM) on performance, intestinal morphology and gene expression in the jejunal mucosa of pigs fed different diets

Influence of the effective microorganisms (EM) on performance, intestinal morphology and gene expression in the jejunal mucosa of pigs fed different diets

Exploring a Possible Link between the Intestinal Microbiota and Feed Efficiency in Pigs

Growth Performance and Post-Weaning Diarrhea in Piglets Fed a Diet Supplemented with Probiotic Complexes

Contact Info

Product

Resources

About