Evaluation of the effects of temperature, light, and UV-C radiation on HSP70A expression in Chlamydomonas reinhardtii

Abstract

In this study, various physical parameters (temperature, light intensity and UV-C radiation) which could be effective in heat shock response on C.reinhardtii by using molecular tools were investigated. In total 256 transformants were obtained, among them 160 transformants had continuous expression while 96 of them had heat-inducible expression. In these transformants, arylsulfatase activities were detected qualitatively and quantitatively. The best two transformants were selected and used in studies. To determine the effect of temperature, the cells were shifted from 23 °C to 35 °C, 37 °C, 40 °C and 42 °C. The heat shock response was induced at all temperatures. In investigating the effect of light intensity, 0, 14, 28, 70, 140 µmol E.m -2 s -1 were used. It was found that the light intensity of 28 µmol E.m -2 s -1 and above increased ARS activity.On the other hand, Ultraviolet C radiation application was carried out for periods of 2, 6 and 12 h, and no significant change in ARS activity was observed. In order to compare the selected arylsulfatase activity results in the study, Real-Time Polymerase Chain Reaction trials were conducted at the transcript level, and parallel results were obtained.As a result of the study, it was determined that the heat shock response was triggered by temperature and light intensity. These might be also important for plant stress and ecological studies.