Evaluation of the effects of boron nitride nanotubes functionalized with gum arabic on the differentiation of rat mesenchymal stem cells

Ferreira, Tiago Hilário; Rocca, Antonella; Marino, Attilio; Mattoli, Virgilio; Sousa, Edésia Martins Barros de; Ciofani, Gianni

doi:10.1039/c5ra05091j

Cited by 19 publications

(11 citation statements)

References 39 publications

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“…The cell viability was more than 80% in all the studied concentrations, indicating no adverse effects on HeLa cultures in terms of metabolic activity after the incubation time. This result is in accordance with others studies that show the good biocompatibility of BNNTs up to 200 μg/mL [ 10 , 32 , 33 ].…”

Section: Resultssupporting

confidence: 93%

“…Recent studies have shown that BNNTs present no apparent toxicity in the concentrations required for their good performance [ 6 ]. It has been demonstrated that BNNTs are internalized by cells [ 7 ], can efficiently deliver DNA [ 8 ], and improve the process of gene transfection [ 9 ] and the differentiation of mesenchymal stem cells [ 10 ]. Furthermore, a theoretical study by Hilder and Hill shows that BNNTs are suitable for drug encapsulation [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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An Assessment of the Potential Use of BNNTs for Boron Neutron Capture Therapy

et al. 2017

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Currently, nanostructured compounds have been standing out for their optical, mechanical, and chemical features and for the possibilities of manipulation and regulation of complex biological processes. One of these compounds is boron nitride nanotubes (BNNTs), which are a nanostructured material analog to carbon nanotubes, but formed of nitrogen and boron atoms. BNNTs present high thermal stability along with high chemical inertia. Among biological applications, its biocompatibility, cellular uptake, and functionalization potential can be highlighted, in addition to its eased utilization due to its nanometric size and tumor cell internalization. When it comes to new forms of therapy, we can draw attention to boron neutron capture therapy (BNCT), an experimental radiotherapy characterized by a boron-10 isotope carrier inside the target and a thermal neutron beam focused on it. The activation of the boron-10 atom by a neutron generates a lithium atom, a gamma ray, and an alpha particle, which can be used to destroy tumor tissues. The aim of this work was to use BNNTs as a boron-10 carrier for BNCT and to demonstrate its potential. The nanomaterial was characterized through XRD, FTIR, and SEM. The WST-8 assay was performed to confirm the cell viability of BNNTs. The cells treated with BNNTs were irradiated with the neutron beam of a Triga reactor, and the apoptosis caused by the activation of the BNNTs was measured with a calcein AM/propidium iodide test. The results demonstrate that this nanomaterial is a promising candidate for cancer therapy through BNCT.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

An Assessment of the Potential Use of BNNTs for Boron Neutron Capture Therapy

et al. 2017

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“…Ciofani et al reported that poly-L lysine functionalized BNNT effectively conjugates fluorescent markers (quantum dots) and targeting moieties (folic acid) thus exhibiting good dispersing ability and cytocompatibility [33][34][35] . Li et al proved that BNNT enhances the differentiation of mesenchymal stem cells into osteoblasts 36 .…”

Section: Introductionmentioning

confidence: 99%

Design of Boron Nitride/Gelatin Electrospun Nanofibers for Bone Tissue Engineering

Nagarajan

Belaid

Pochat-Bohatier

et al. 2017

ACS Appl. Mater. Interfaces

147

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Gelatin is a biodegradable biopolymer obtained by collagen denaturation, which shows poor mechanical properties. Hence, improving its mechanical properties is very essential toward the fabrication of efficient nontoxic material for biomedical applications. For this aim, various methods are employed using external fillers such as ceramics or bioglass. In this report, we introduce boron nitride (BN)-reinforced gelatin as a new class of two-dimensional biocompatible nanomaterials. The effect of the nanofiller on the mechanical behavior is analyzed. BN is efficiently exfoliated using the biopolymer gelatin as shown through Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). The exfoliated BN reinforces gelatin electrospun fibers, which results in an increase in the Young's modulus. The Electrospun Mats (ESM) are stable after the glutaraldehyde cross-linking, and the fibrous morphology is preserved. The cross-linked gelatin/BN ESM is highly bioactive in forming bonelike hydroxyapatite as shown by scanning electron microscopy. Due to their enhanced mineralization ability, the cross-linked ESM have been tested on human bone cells (HOS osteosarcoma cell line). The cell attachment, proliferation, and biocompatibility results show that the ESM are nontoxic and biodegradable. The analysis of osteoblast gene expression and the measurement of alkaline phosphatase activity confirm that these materials are suitable for bone tissue engineering.

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“…Overall, it was reported that BNNTs were able to enhance the osteogenesis of MSCs, which showed potential in bone regeneration in orthopaedic applications [52]. Ferreira et al [60] incubated GA-BNNTs in MSCs to investigate the cytocompatibility. The cell viability and proliferation were not affected, up to 20 µg•mL −1 of GA-BNNTs [60].…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

In Vitro and In Vivo Cytotoxicity of Boron Nitride Nanotubes: A Systematic Review

Kakarla

Kong

2022

Nanomaterials

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Boron nitride nanotubes (BNNTs) are an exciting class of nanomaterials due to their unique chemical and physical characteristics. In recent decades, BNNTs have gained huge attention in research and development for various applications, including as nano-fillers for composites, semiconductor devices, hydrogen storage, and as an emerging material in biomedical and tissue engineering applications. However, the toxicity of BNNTs is not clear, and the biocompatibility is not proven yet. In this review, the role of BNNTs in biocompatibility studies is assessed in terms of their characteristics: cell viability, proliferation, therapeutic outcomes, and genotoxicity, which are vital elements for their prospective use in biomedical applications. A systematic review was conducted utilising the databases Scopus and Web of Science (WOS) (2008–2022). Additional findings were discovered manually by snowballing the reference lists of appropriate reviews. Only English-language articles were included. Finally, the significant analysis and discussion of the chosen articles are presented.

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Evaluation of the effects of boron nitride nanotubes functionalized with gum arabic on the differentiation of rat mesenchymal stem cells

Cited by 19 publications

References 39 publications

An Assessment of the Potential Use of BNNTs for Boron Neutron Capture Therapy

An Assessment of the Potential Use of BNNTs for Boron Neutron Capture Therapy

Design of Boron Nitride/Gelatin Electrospun Nanofibers for Bone Tissue Engineering

In Vitro and In Vivo Cytotoxicity of Boron Nitride Nanotubes: A Systematic Review

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