Evaluation of the effects of low concentrations of aridanin isolated from Tetrapleura tetraptera Taub. (Mimosaceae) on the growth and egg production of Biomphalaria glabrata say and Lymnaea columella say

Abstract

The effects o f low concentrations of aridanin ( O . B , 0.25, 0.5, and 1.0ppm) on the growth and egg production of Biomphalaria glabrata and Lymnaea columella were compared with the effects of low concentrations of niclosamide (0.025, 0.05, and 0.10 ppm). Aridanin and niclosamide caused significant reduction in the egg production and growth of B . glabrata and L. columella, indicating that aridanin has a potential use in schistosomiasis control if used in slow-release formulations. The poor chemical stability of aridanin in clear water suggests that its effectiveness as a molluscicide in the field will depend on the speed of its destructive action on snails.

The Effect of Aridanin Isolated from Tetrapleuratetraptera and Serotonin on the Isolated Gastrointestinal Tract Smooth Muscles of Biomphalaria glabrata and Uptake of Calcium

“…If skinning of any membrane is achieved, the cell will allow the entry of any ion or even a micromolecule (20); so the membrane potential can thus be completely abolished. The concentration of aridanin used in this study is low (4 X 10-7-3 X 10-6 M), and the fact that aridanin exerts its effects even at these sublethal concentrations suggests that an aridanin-induced pertubation of membrane may be related to its molluscicidal activity (1-3), its effect on the growth and egg production of B. glabrata and Lymnaea columella (11), and its effect on carbohydrate and protein content of B. glabrata (5).…”

“…Sublethal concentrations of aridanin were used in our studies on the effect of aridanin on the biology of B. glabrata (5,11). The enhancement of the rhythmicity of the gut of the snail was not mediated by endogenous substances like serotonin, acetylcholine, or catecholamines, as antagonists of these substances had no effect on the action produced by aridanin.…”

“…Controls with no aridanin were included. The test solutions were changed every 3-4 days because aridanin has been found to be inactive in solution after standing for up to 5 days (11). Calcium concentration was determined using the EDTA-titration method of Golterman et al (12).…”