Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy

Došenović, Milan; Radaković, Milena; Vučićević, Miloš; Vejnović, Branislav; Vasiljević, Maja; Marinković, Darko; Stanimirović, Zorica

doi:10.1556/004.2020.00058

Cited by 3 publications

(2 citation statements)

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“…Leffa et al 31 showed that xylazine-ketamine administration resulted in DNA damage in the liver and kidneys of rats. Došenović et al 32 conducted a study on turtles and found that medetomidine-ketamine anesthesia did not cause DNA damage. Similarly, in this study, it was determined that xylazine-ketamine administration caused higher levels of DNA damage in the liver and kidneys compared to medetomidine-ketamine administration ( Fig.…”

Section: Discussionmentioning

confidence: 99%

The effects of repeated doses of xylazine-ketamine and medetomidineketamine anesthesia on DNA damage in the liver and kidney

Sancak

2023

Acta Cir. Bras.

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Purpose: This study evaluated the DNA damage caused by repeated doses of xylazine-ketamine and medetomidine-ketamine anesthesia in the liver and kidneys. Methods: In this study, 60 rats were used. The rats were divided into group 1 (xylazine-ketamine), and group 2 (medetomidine-ketamine), and these anesthetic combinations were administered to the rats at repeated doses with 30-min intervals. The effects of these anesthetic agents on the tumor necrosis factor-alpha gene for DNA damage were investigated. Results: According to the gene expression results, it was observed that a single dose of xylazine-ketamine was 2.9-fold expressed, while first and second repeat doses did not show significant changes in expression levels. However, in the case of the third repetition, it was observed to be 3.8-fold overexpressed. In the case of medetomidine-ketamine administration, it was observed that a single-dose application resulted in a 1.04-fold expression, while the first and the third repeat doses showed a significant down expression. The samples from the second repeat dose administration group were found to have insignificant levels of expression. Conclusions: This study can contribute to understanding the safe anesthetic combination in research and operations in which xylazine-ketamine and medetomidine-ketamine combinations are used.

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Section: Discussionmentioning

confidence: 99%

The effects of repeated doses of xylazine-ketamine and medetomidineketamine anesthesia on DNA damage in the liver and kidney

Sancak

2023

Acta Cir. Bras.

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“…In this study, all the antioxidant defenserelated traits, CAT, were downregulated (i.e., SOD, GSH/GSSG, and T-AOC) in turtles from low latitudes after being exposed to heat waves (Figure 2). Widely depressed antioxidant defenses (i.e., SOD, GPx, and T-AOC) in low-latitude turtles may limit their ability to eliminate ROS and prevent oxidative damage, such as the production of MDA (Došenović et al, 2021;Pinya et al, 2021). The effects of SOD, CAT, and GPx activity on the elimination of MDA have been widely demonstrated in vertebrates, including fishes (Fakhereddin and Doğan, 2021), reptiles (Han et al, 2020;Kou et al, 2021;Pinya et al, 2021), birds (Altan et al, 2003), and mammals (Wei et al, 2016).…”

Section: Higher Oxidative Damage By Heat Waves In Low-latitude Turtlesmentioning

confidence: 99%

Heat wave induces oxidative damage in the Chinese pond turtle (Mauremys reevesii) from low latitudes

Tao

Ou²,

Wu³

et al. 2022

Front. Ecol. Evol.

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IntroductionGlobal warming has led to frequent heat waves, causing global organisms to face severe survival challenges. However, the way in which heat waves threaten the fitness and survival of animals remains largely unclear. Oxidative damage and immunity are widely considered the link between heat waves and threats to animals.MethodsTo evaluate the oxidative damage caused by heat waves and to reveal the physiological resistance to heat waves by the antioxidant defense of animals from different latitudes, we exposed both high-latitude (Zhejiang) and low-latitude (Hainan) populations of Chinese pond turtle (Mauremys reevesii) to simulate heat waves and a moderate thermal environment for 1 week, respectively. Next, we compared the oxidative damage by malondialdehyde (MDA) and antioxidant capacity by superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and total antioxidant capacity (T-AOC) in the liver tissues and evaluated the innate immunity by serum complement protein levels (C3, C4) and lysozyme activity in plasma of turtles.Results and discussionWe found that heat waves significantly increased the content of MDA and the activity of CAT, whereas it decreased the activity of SOD, T-AOC, and GSH/GSSG in turtles from low latitudes. Furthermore, heat waves increased CAT activity but decreased GSH/GSSG in turtles from high latitudes. Although the turtles from high latitudes had higher levels of innate immunity, the heat waves did not affect the innate immunity of C3, C4, or lysozyme in either population. These results indicate that the low-latitude population suffered higher oxidative damage with lower antioxidant capacities. Therefore, we predict that Chinese pond turtles from low latitudes may be more vulnerable to heat waves caused by climate warming. This study reveals the physiological and biochemical resistance to heat waves in Chinese pond turtles from different latitudes and highlights the importance of integrative determination of fitness-related responses in evaluating the vulnerability of ectotherms from different latitudes to climate warming.

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A comparison of anesthesia induction by two different administration routes and doses of ketamine and medetomidine in red-eared sliders (Trachemys scripta elegans)

Lutvikadić

MAKSIMOVIĆ

2024

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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This study aimed to assess the efficiency of ketamine and medetomidine by two different doses and routes on anesthesia depth and cardiac stability in red-eared sliders. Each turtle was anesthetized two times, with seven days wash-out period. Induction of anesthesia consisted of a bolus combination of ketamine (10 mg/kg) and medetomidine (0.2 mg/kg) administered in the left brachial biceps in the intramuscular protocol, or a bolus combination of ketamine (20 mg/kg) and medetomidine (0.2 mg/kg) administered in subcarapacial sinus after clear blood presence confirmation in the intravenous protocol. Vital signs, reaction on the skin palpation, manual mouth opening for endotracheal intubation, palpebral and cloacal reflex, and the withdrawal reflex of the front and hind limbs were measured and recorded every 5 minutes for 60 minutes after anesthesia injection. Atipamezole (1 mg/kg) was administered in the right brachial biceps one hour after ketamine and medetomidine administration. Needle insertion and possible painful reactions to drug administration were also evaluated and recorded. Obtained data were analyzed for normality and paired t-tests, Wilcoxon, or McNamar tests were performed where appropriate. The values of P ≤ 0.05 were considered significant. A significantly less pronounced decrease in heart rate was observed with intravenous anesthesia protocol. Both protocols recorded complete anesthesia recovery 60 minutes after intramuscular atipamezole administration. A ketamine-medetomidine dose combination administered intravenously provides a more stable and consistent anesthetic plane in red-eared sliders than ketamine-medetomidine administered intramuscularly.

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Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy

Cited by 3 publications

References 36 publications

The effects of repeated doses of xylazine-ketamine and medetomidineketamine anesthesia on DNA damage in the liver and kidney

The effects of repeated doses of xylazine-ketamine and medetomidineketamine anesthesia on DNA damage in the liver and kidney

Heat wave induces oxidative damage in the Chinese pond turtle (Mauremys reevesii) from low latitudes

A comparison of anesthesia induction by two different administration routes and doses of ketamine and medetomidine in red-eared sliders (Trachemys scripta elegans)

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