Evaluation of the effects of different liquid inoculant formulations on the survival and plant-growth-promoting efficiency of Rhodopseudomonas palustris strain PS3

Lee, Sook-Kuan; Lur, Huu‐Sheng; Lo, Kai-Jiun; Cheng, Kuan‐Chen; Chuang, Chun-Chao; Tang, Shiueh-Jung; Yang, Zhi-Wei; Liu, Chi-Te

doi:10.1007/s00253-016-7582-9

Cited by 54 publications

(50 citation statements)

References 47 publications

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“…Hence it is necessary to develop strategies for effective inoculation methods, so that bacteria of interest gain advantage in colonization efficiency over others. Product quality, compatibility, and stability determine effective colonization and consistent performance of the inoculum under field conditions ( Lee et al, 2016 ).…”

Section: Strategies For Improving Rhizosphere Colonization By Pgpr Inmentioning

confidence: 99%

Plant Growth-Promoting Rhizobacteria: Context, Mechanisms of Action, and Roadmap to Commercialization of Biostimulants for Sustainable Agriculture

et al. 2018

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Microbes of the phytomicrobiome are associated with every plant tissue and, in combination with the plant form the holobiont. Plants regulate the composition and activity of their associated bacterial community carefully. These microbes provide a wide range of services and benefits to the plant; in return, the plant provides the microbial community with reduced carbon and other metabolites. Soils are generally a moist environment, rich in reduced carbon which supports extensive soil microbial communities. The rhizomicrobiome is of great importance to agriculture owing to the rich diversity of root exudates and plant cell debris that attract diverse and unique patterns of microbial colonization. Microbes of the rhizomicrobiome play key roles in nutrient acquisition and assimilation, improved soil texture, secreting, and modulating extracellular molecules such as hormones, secondary metabolites, antibiotics, and various signal compounds, all leading to enhancement of plant growth. The microbes and compounds they secrete constitute valuable biostimulants and play pivotal roles in modulating plant stress responses. Research has demonstrated that inoculating plants with plant-growth promoting rhizobacteria (PGPR) or treating plants with microbe-to-plant signal compounds can be an effective strategy to stimulate crop growth. Furthermore, these strategies can improve crop tolerance for the abiotic stresses (e.g., drought, heat, and salinity) likely to become more frequent as climate change conditions continue to develop. This discovery has resulted in multifunctional PGPR-based formulations for commercial agriculture, to minimize the use of synthetic fertilizers and agrochemicals. This review is an update about the role of PGPR in agriculture, from their collection to commercialization as low-cost commercial agricultural inputs. First, we introduce the concept and role of the phytomicrobiome and the agricultural context underlying food security in the 21st century. Next, mechanisms of plant growth promotion by PGPR are discussed, including signal exchange between plant roots and PGPR and how these relationships modulate plant abiotic stress responses via induced systemic resistance. On the application side, strategies are discussed to improve rhizosphere colonization by PGPR inoculants. The final sections of the paper describe the applications of PGPR in 21st century agriculture and the roadmap to commercialization of a PGPR-based technology.

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Section: Strategies For Improving Rhizosphere Colonization By Pgpr Inmentioning

confidence: 99%

Plant Growth-Promoting Rhizobacteria: Context, Mechanisms of Action, and Roadmap to Commercialization of Biostimulants for Sustainable Agriculture

et al. 2018

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“…We assayed SAGE in the phylogenetically distant alphaproteobacterium, Rhodopseudomonas palustris 60 . Able to grow under both anaerobic and aerobic conditions in heterotrophic, phototrophic, and chemolithotrophic modes of growth, R. palustris has one of the most versatile metabolic repertoires known bacteria, and are also often PGPR [61][62][63][64] .…”

Section: Sage Enables Efficient Genome Engineering In Diverse Bacteriamentioning

confidence: 99%

The SAGE genetic toolkit enables highly efficient, iterative site-specific genome engineering in bacteria

Elmore

Francis

et al. 2020

Preprint

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Sustainable enhancements to crop productivity and increased resilience to adverse conditions are critical for modern agriculture, and application of plant growth promoting rhizobacteria (PGPR) is a promising method to achieve these goals. However, many desirable PGPR traits are highly regulated in their native microbe, limited to certain plant rhizospheres, or insufficiently active for agricultural purposes. Synthetic biology can address these limitations, but its application is limited by availability of appropriate tools for sophisticated, high-throughput genome engineering that function in environments where selection for DNA maintenance is impractical. Here we present an orthogonal, Serine-integrase Assisted Genome Engineering (SAGE) system, which enables iterative, site-specific integration of up to 10 different DNA constructs at efficiency on par or better than replicating plasmids. SAGE does not require use of replicating plasmids to deliver recombination machinery, and employs a secondary serineintegrase to excise and recycle selection markers. Furthermore, unlike the widely utilized pBBR1 origin, DNA transformed using SAGE is stable without selection. We highlight SAGE's utility by constructing a 287-member constitutive promoter library with a ~40,000-fold dynamic range in P. fluorescens SBW25. We show that SAGE functions robustly in diverse ⍺and "-proteobacteria, thus providing evidence that it will be broadly useful for engineering industrial or environmental bacteria.

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“…Different technological approaches have been used in order to formulate more efficient inoculants, which must be combined with suitable application methods in the agricultural environment (1,(6)(7)(8). An adequate formulation must guarantee a minimum number of viable microbial cells, and once applied in the soil-plant system, establish itself in the rhizosphere or plant-host body.…”

Section: Introductionmentioning

confidence: 99%

“…An adequate formulation must guarantee a minimum number of viable microbial cells, and once applied in the soil-plant system, establish itself in the rhizosphere or plant-host body. Thus, the inoculant formulation must guarantee the survival of the cells for a period that allows their shelf-life commercialization (6). Also, the rapid decline of the introduced microbial population, reduce competitiveness, and plant colonization capability (9,10).…”

Section: Introductionmentioning

confidence: 99%

Mutualistic interaction withTrichoderma longibrachiatumUENF-F476 boosted plant growth-promotion ofSerratia marcescensUENF-22GI

Reis

Alves

Santos

et al. 2020

Preprint

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BACKGROUND: A plethora of bacteria-fungal interactions occurs on the extended fungal hyphae network in soil. The mycosphere of saprophytic fungi can serve as a bacterial niche boosting their survival, dispersion, and activity. Such ecological concepts can be converted to bioproducts for sustainable agriculture. Accordingly, we tested the hypothesis that the well-characterized beneficial bacterium Serratia marcescens UENF-22GI can enhance their plant growth-promoting properties by combination with Trichoderma longibrachiatum UENF-F476. RESULTS: The colony and cell interactions demonstrated S. marcescens and T. longibrachiatum compatibility. Bacteria cells were able to attach, forming aggregates- biofilms and migrates through fungal hyphae network. Bacterial migration through growing hyphae was confirmed using two-compartment Petri dishes assay. Fungal inoculation increased the bacteria survival rates into the vermicompost substrate over the experimental time. Also, in vitro indolic compound, phosphorus, and zinc solubilization bacteria activities increased in the presence of the fungus. In line with the ecophysiological bacteria fitness, tomato and papaya plantlet growth was boosted by bacteria-fungi combination applied under plant nursery conditions. CONCLUSION: Mutualistic interaction between mycosphere-colonizing bacterium S. marcescens UENF-22GI and the saprotrophic fungi T. longibrachiatum UENF-F467 increased the ecological fitness of the bacteria alongside with beneficial potential for plant growth. A proper combination and delivery of compatible beneficial bacteria-fungus represent an open avenue for biological enrichment of plant substrates technologies in agricultural systems.

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Evaluation of the effects of different liquid inoculant formulations on the survival and plant-growth-promoting efficiency of Rhodopseudomonas palustris strain PS3

Cited by 54 publications

References 47 publications

Plant Growth-Promoting Rhizobacteria: Context, Mechanisms of Action, and Roadmap to Commercialization of Biostimulants for Sustainable Agriculture

Plant Growth-Promoting Rhizobacteria: Context, Mechanisms of Action, and Roadmap to Commercialization of Biostimulants for Sustainable Agriculture

The SAGE genetic toolkit enables highly efficient, iterative site-specific genome engineering in bacteria

Mutualistic interaction withTrichoderma longibrachiatumUENF-F476 boosted plant growth-promotion ofSerratia marcescensUENF-22GI

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