Evaluation of the effects of weak and moderate static magnetic fields on the characteristics of human low density lipoprotein in vitro

Abdi, Soheila; Dorranian, Davoud; Razavi, Àmirnader Emami; Naderi, Gholam Ali; Boshtam, Maryam; Ghorannevis, M.

doi:10.1002/bem.21779

Cited by 9 publications

(6 citation statements)

References 47 publications

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“…Before enzyme activity measurement, serum samples (inside a quartz cuvette) were put in the centre of the solenoid for certain times. The produced SMF and EMF at the exact site of cuvette location was measured using a digital teslameter with a 3-D sensor (Holaday, Eden Prairie, MN) (21,22).…”

Section: Methodsmentioning

confidence: 99%

Effects of static and electromagnetic fields on human serum paraoxonase-1 activity in vitro

Abdi

2018

JBRMS

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Introduction: In recent years the relationship between electromagnetic fields and coronary artery disease is attracted a considerable attention. Low density lipoprotein (LDL) oxidation is the initial step in the development of atherosclerosis. Paraoxonase1 (PON1) protects LDL and High density lipoprotein (HDL) against oxidative processes, thus preventing the formation of atherogenic (oxidized-LDL) ox-LDL molecules. In this study we investigated the effects of static magnetic fields (SMFs) and extremely low frequency electromagnetic fields (ELFEMFs) on PON1 activity as one of the independent risk factors for cardiovascular disease. Materials and methods: Pooled serum sample of 20 healthy men were exposed to SMFs and EMFs flux densities of 0.125, 0.25, 0.5, 1, 2, 3 and 4 mT for 60, 120 and 180 minutes at 25°C and then PON1 activity was measured spectrophotometrically using paraoxon as substrate. Results: EMFs of 0.125-1 mT had no effect on PON1 activity. Exposure to magnetic flux density of 2 mT leads to a significant increase in PON1 activity in 1 hour (P <0.05). Magnetic flux density of 3 and 4 mT, after 1hour of exposure, lead to an increase of PON1 activity to 1.2% and 2.8%, respectively (P <0.01). Conclusion: PON1 activity is influenced by a variety of agents like environmental, pharmacological, and lifestyle factors as well as age and sex. According to the finding of this study ELF-EMFs can alternate the serum activity of PON1 in vitro. If this effect of EMFs on PON1 activity has proven in vivo, it can be considered as an effective factor in coronary artery disease.

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Section: Methodsmentioning

confidence: 99%

Effects of static and electromagnetic fields on human serum paraoxonase-1 activity in vitro

Abdi

2018

JBRMS

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“…The device used to create an alternative magnetic field was solenoid which was described in our previous report (19). The conditions were the same for the cells inside the solenoid.…”

Section: Exposure Systemmentioning

confidence: 99%

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

et al. 2022

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: The biological effects of electromagnetic fields (EMF), in particular their beneficial or adverse impacts on the promotion and progression of cancer, have attracted considerable attention. C-Myc has a critical regulatory role in cell transformation and causes metabolic changes, that accompany malignant transformation. Observations have shown that c-Myc expression can be altered by circular RNAs (circRNA). In this study, the changes in the expression of c-Myc and circ-CCDC66 in the AGS cell line after the extremely low-frequency magnetic fields (ELF-MF) exposure were investigated. The AGS cells were exposed to different magnetic flux densities for 18 h, continuously and discontinuously (2h on/2h off). To evaluate the expression changes of c-Myc and circ-CCDC66 real-time PCR was used. The results showed that discontinuous magnetic fields could reduce c-Myc expression. Continuous exposure of 0.25 mT could reduce the c-Myc expression, but with increasing the magnetic flux density c-Myc was upregulated. The expression level of circ-CCDC66 decreased under exposure to continuous and discontinuous ELF-MFs. The difference between the results of c-Myc under the influence of magnetic fields may be described by the hypothesis of the window effect of the fields. Our results indicated that ELF-MFs can induce changes in c-Myc and circ-CCDC66 expression.

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“…A solenoid cylinder with a diameter of 12 cm, height of 30 cm, and 1200 turns was used to produce electromagnetic fields (6). An AC power supply (TDGC2, 220v, 50 -60 Hz Delta International Electric, Shanghai, China) was applied to produce various electromagnetic intensities using city power with a frequency of 50 Hz (19).…”

Section: Exposure Systemmentioning

confidence: 99%

“…Therefore, the intensity of the magnetic field obtained from these devices varies between 0.1 and 8 mT, depending on the current intensity and the distance from the device (5). They can lead to various biological effects depending on the density, frequency, and duration of their radiation (6). Electromagnetic waves have been reported to cause breast cancer Copyright © 2021, Author(s).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the PTEN and circRNA-CDR1as Gene Expression Changes in Gastric Cancer and Normal Cell Lines Following the Exposure to Weak and Moderate 50 Hz Electromagnetic Fields

et al. 2021

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Background: Attention to the electromagnetic exposure as a targeted tumor therapy has been recently increasing. Objectives: The aim of the current study was to investigate the effect of continuous and discontinuous electromagnetic fields on cell viability as well as phosphatase and tensin homolog (PTEN) and circular (circ)-RNA CDR1as genes expression in the normal and gastric cancer (GC) cell lines. Methods: After preparing gastric cancer cell lines (AGS) and normal cells (HU02 line), they were exposed to magnetic flux densities of 0.25, 0.5, 1, and 2 mT continuously and discontinuously (1h on/1h off) for 18 hours. The 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cell viability. In addition, after designing the primers, the expression of the PTEN and circ-CDR1as genes was studied using the real-time polymerase chain reaction (real-time-PCR) technique. The results were analyzed using SPSS software version 25. Results: The exposed normal and tumor cells to discontinuous electromagnetic fields resulted in increasing of cell survival rate in both normal and tumor cells. In contrast, the exposure of continuous electromagnetic field showed no effect on the viability of the normal and tumor cells at intensities of 0.25, 0.5, and 1 mT. The electromagnetic field showed a significant effect on the expression of the circ-CDR1as gene and this effect depended on the intensity of the electromagnetic field used and the cell type. We have found that the activity of PTEN gene in the normal and tumor cells increased and decreased with increasing intensity of discontinuous electromagnetic field, respectively. Conclusions: In general, the effect of electromagnetic field on gastric cancer seems to depend on the kind of exposure as well as an extent of intensity and can be used for cancer therapeutic purposes. However, more research is needed on this subject.

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Evaluation of the effects of weak and moderate static magnetic fields on the characteristics of human low density lipoprotein in vitro

Cited by 9 publications

References 47 publications

Effects of static and electromagnetic fields on human serum paraoxonase-1 activity in vitro

Effects of static and electromagnetic fields on human serum paraoxonase-1 activity in vitro

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

Evaluation of the PTEN and circRNA-CDR1as Gene Expression Changes in Gastric Cancer and Normal Cell Lines Following the Exposure to Weak and Moderate 50 Hz Electromagnetic Fields

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