Evaluation of the Effects of the Extract of Vernonia amygdalina on Fungi Associated with Infected Tomatoes (Lycopersicon esculentum) in Jos North Local Government Area, Plateau State, Nigeria

John, W. C.; Anyanwu, Nneoma Confidence JeanStephanie; Ayisa, Timothy

doi:10.9734/arrb/2016/23698

Cited by 5 publications

(6 citation statements)

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“…The percentage of fungal inhibition in amended PDA medium was also dependent on concentration, and the most significant reduction in mycelial growth was obtained with the highest concentration of crude extract. A similar result was observed by other researchers using ethanol leaf extract of V. amygdalina against tomato diseases [16]. This could be due to the level of composition antifungal compounds obtained from the crude extract.…”

Section: Discussionsupporting

confidence: 89%

“…To date, most researchers have focused on V. amygdalina crude extract in order to uncover its potential as an antifungal agent for the management of plant disease. Recent findings found that V. amygdalina ethanol extracts showed a good ability to inhibit postharvest fungal pathogens Rhizopus stolonifer and Fusarium moniliforme [ 16 , 17 ]. In another study, in an in vitro test using an ethanol crude extract of V. amygdalina at 300 mg/mL, the growth of Cercosporella persica and Curvularia lunatus were completely inhibited [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Yusoff

Haron

Mohamed

et al. 2020

Biology

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Gray mold disease caused by Botrytis cinerea is a damaging postharvest disease in tomato plants, and it is known to be a limiting factor in tomato production. This study aimed to evaluate antifungal activities of Vernonia amygdalina leaf extracts against B. cinerea and to screen the phytochemical compound in the crude extract that had the highest antifungal activity. In this study, crude extracts of hexane, dichloromethane, methanol, and water extracts with concentration levels at 100, 200, 300, 400, and 500 mg/mL were shown to significantly affect the inhibition of B. cinerea. Among the crude extracts, dichloromethane extract was shown to be the most potent in terms of antifungal activities. The SEM observation proved that the treatment altered the fungal morphology, which leads to fungal growth inhibition. For the in vivo bioassay, the fruits treated with dichloromethane extract at 400 and 500 mg/mL showed the lowest disease incidence with mild severity of infection. There were 23 chemical compounds identified in V. amygdalina dichloromethane extract using GCMS analysis. The top five major compounds were dominated by squalene (16.92%), phytol (15.05%), triacontane (11.31%), heptacosane (7.14%), and neophytadiene (6.28%). Some of these significant compounds possess high antifungal activities. This study proved that V. amygdalina from dichloromethane extract could be useful for inhibiting gray mold disease on tomato fruit and has potential as a natural antifungal agent.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Yusoff

Haron

Mohamed

et al. 2020

Biology

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“…The method of John et al [17] was used. Small portions of freshly grown colony were picked from the plate into a glass slide using a sterile inoculating needle.…”

Section: Fungi Identificationmentioning

confidence: 99%

“…The collected filtrate was evaporated to dryness using a rotary evaporator and a drying cabinet. The percentage yield of the extract was determined and the extract was transferred into a sterile sample container and preserved in the refrigerator [17].…”

Section: Preparation Of Lemongrass Extractmentioning

confidence: 99%

“…Fresh Mycelia cell were picked from cultures plates and used for the inoculation of cut part. The cut portions were sealed with petroleum jelly to prevent contamination by other microorganisms [17]. The inoculated tubers and the control (un-inoculated) were placed separately in sterile polythene bags containing cotton wool soaked in sterile distilled water to provide humid environment [19].…”

Section: Pathogenicity Testmentioning

confidence: 99%

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Bioassay of Lemongrass on Fungi Pathogen Associated with Cassava Tubers Rot in Farin Gada Market, Jos

John

Oke

Ishaya

et al. 2019

MRJI

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The aim of this study was to determine the effects of Lemongrass on fungal pathogen associated with cassava tuber rot. The study was carried out in the biology laboratory of the Federal College of Forestry Jos, Plateau state from March to May, 2019. Rotten and healthy cassava tubers were collected separately from Farin-Gada market Jos, fungi species were isolated from rotten cassava tubers by direct inoculation of the spoiled part on sterile Potato Dextrose Agar medium and incubated for 3-5 days, the isolated fungi were identified microscopically and macroscopically. The identified fungi were used for pathogenicity test. The antifungal effect of different concentrations of ethanol extract of lemongrass was investigated. Data collected were analyzed using one way ANOVA and the means were separated using Least Significant Difference (LSD) at (p≥0.05). The fungi isolated include, Fusarium sp, Penicillium sp, Geotrichum candidum, and Aspergillus flavus. The frequency of occurrence of the isolated fungi indicated Fusarium sp, Penicillium sp, Geotrichum candidum, and Aspergillus flavus had 30, 15, 35 and 20% respectively. 20 mL of the tested extract gave the highest inhibition of 19.07, 20.57, 18.17 mL and 18.00 mL on Fusarium spp, Penicillium spp, Geotrichum candidum and Aspergillus flavus respectively. At the 5th day of incubation the results of the pathogenicity showed that Aspergillus flavus gives the highest deterioration of 9.17 mm. The length of deterioration showed significant difference. The lemongrass extract indicated anti-fungal effect on the fungal isolates, therefore could be used to control cassava tuber rot caused by fungi.

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Antifungal potentials of some botanicals on Sclerotium rolfsii schum., the causal pathogen of damping-off of Gmelina arborea Roxb. in Ibadan, Southwestern Nigeria

Nwogwugwu

Batcho

2019

J Plant Dis Prot

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Evaluation of the Effects of the Extract of Vernonia amygdalina on Fungi Associated with Infected Tomatoes (Lycopersicon esculentum) in Jos North Local Government Area, Plateau State, Nigeria

Cited by 5 publications

References 0 publications

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Bioassay of Lemongrass on Fungi Pathogen Associated with Cassava Tubers Rot in Farin Gada Market, Jos

Antifungal potentials of some botanicals on Sclerotium rolfsii schum., the causal pathogen of damping-off of Gmelina arborea Roxb. in Ibadan, Southwestern Nigeria

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