Evaluation of the effect of CaD on the bone structure and bone metabolic changes in senile osteoporosis rats based on MLP–ANN methods

Tang, Xian‐Liang; Yingying, Gao; Chen, Yuheng; Li, Xiaoxi; Yu, Ping; Ma, Zitong; Liu, Renhui

doi:10.1039/c9fo01322a

Cited by 11 publications

(9 citation statements)

References 51 publications

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“…BMD of each right femur was measured with a dual-energy X-ray absorptiometry system (DEXA, Lexxos-2000, Medlink, France) equipped with appropriate software for bone assessment in small animals according to our previous report [21].…”

Section: Bone Mineral Density (Bmd)mentioning

confidence: 99%

“…After BMD measurement, the biomechanical properties of the right femur were assessed using a diaphysis bending test on a WD-1 Universal Testing Apparatus (KeXin Testing Machine Co., Ltd., Changchun, China) that was equipped with calculation and analysis software according to our previous report [21]. Loading-displacement curves were recorded on-line and analyzed to determine the ultimate load and displacement at ultimate.…”

Section: Bone Biomechanical Testingmentioning

confidence: 99%

“…The left femur were lysed on ice in the RIPA buffer with protease inhibitor cocktail for 40 min to extract total proteins. The proteins were analyzed with a bicinchoninic acid (BCA) protein assay kit and 30 μg/lane were used for WB analysis as previously described [21], using antibodies, including TGF-β1 (1:4000), RANKL (1:500), Wnt5a (1:4000), Atp6v0d2 (1:500), M-CSF (1:500), OPG (1:5000) and BMP-2 (1:2000), and internal reference protein of β-Tubulin (1:1000).…”

Section: Western Blot (Wb)mentioning

confidence: 99%

“…In our previous study, a MLP-ANN model had been established according to the dynamic changes of relevant parameters in male rats aged 3, 6, 9, 12, 15 and 17 months using SPSS 21.0 software (SPSS Inc., Chicago, USA) [21]. The agreement between the experimental data and the predicted value of MLP is very high, and the model is credible, so we used the constructed MLP model to evaluate the comprehensive effect of EF and LLF in single use or combined use on SOP rats.…”

Section: Evaluatin Based On Mlp-annmentioning

confidence: 99%

“…Multi-layer perception (MLP) is an important nonlinear multi-factor analysis method and one of the most widely used ANN models. In our previous study [21], a credible MLP-ANN model has been designed according to the dynamic changes of osteoporosis parameters in SOP rats aged from 6 to 17 months. In the present study, we focused on exploring the changes of the parameters related to bone remodeling in SOP rats, used the constructed MLP-ANN model to evaluate and compare the therapeutic effect of EF and LLF in single use or in combination, and provide experimental basis for the treatment of clinical SOP.…”

Section: Introductionmentioning

confidence: 99%

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Systemic osteoprotective effects of Epimedii Folium and Ligustri Lucidi Fructus in senile osteoporosis rats by promoting the osteoblastogenesis and osteoclastogenesis based on MLP-ANN model

Tang

Yingying

et al. 2020

Chin Med

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Background: Senile osteoporosis (SOP), which is caused by unbalanced bone remodeling, leads to significant economic and societal burdens globally. The combination of Epimedii Folium (EF) and Ligustri Lucidi Fructus (LLF) serves as a commonly-used prescription for SOP in Traditional Chinese Medicine (TCM). This study aimed to evaluate the osteoprotective effects of EF and LLF in combination on SOP rats based on the constructed multilayer perception (MLP)-artificial neural network (ANN) model. Methods: 15 month old male Sprague-Dawley rats were administrated with EF, LLF or the combination of EF and LLF (EF&LLF) for 2 months, while 17 month old rats were used as the aging control group. All the rats were anesthetized with 25% ethyl carbamate, then their serum liver and bone tissues were taken. We detected bone mass, bone mineral density (BMD), biomechanics and the microstructure of bone trabecula by micro-CT and H&E staining to evaluate the degree of osteoporosis. Blood lipids and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyl transferase (GGT) and liver pathology were use to assess the side effects of drugs. Levels of alkaline phosphatase (ALP) and Tartrate-resistant acid phosphatase (TRACP) and the ratio of ALP to TRACP both in serum and bone were measured for the evaluation of bone turnover rate. The bone mRNA and protein expression of osteoprotegerin (OPG), nuclear factor-kappa B ligand (RANKL), macrophage colony-stimulating factor (M-CSF), d2 isoform of vacuolar (H +) ATPase (ATP6V0d2), insulin-like growth factor (IGF-1), bone morphogenetic protein-2 (BMP2), M-CSF, Wnt5a, transforming growth factor-β1 (TGF-β1) were detected for evaluating bone metabolism. Results: The results showed that EF&LLF improved bone mass and bone quality by preventing bone loss, increasing maximal load as well as protecting the micro-structural retrogressive change of trabecular bone in SOP rats; ameliorated the steatosis in the liver and decreased blood lipids and serum ALT, AST and GGT; enhanced bone remodeling by stimulating the expression of ALP and TRACP. At the molecular levels, EF&LLF stimulated the osteoclastogenesis by upregulating the protein and mRNA expression of OPG, RANKL, M-CSF and ATP6V0d2; meanwhile, EF&LLF stimulated

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Section: Bone Mineral Density (Bmd)mentioning

confidence: 99%

Section: Bone Biomechanical Testingmentioning

confidence: 99%

Section: Western Blot (Wb)mentioning

confidence: 99%

Section: Evaluatin Based On Mlp-annmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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