Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model

Abstract

Collagen is an extracellular matrix protein present in the skin, tendon, cartilage and bone. Collagen peptides (CP) are produced by the hydrolysis of gelatin (heat-denatured collagen) by proteases and are utilized as a component of nutraceuticals. The current study investigated the effect of CP on the articular cartilage of OA by evaluating the serum levels of biomarkers (CTX–II for type II collagen degradation and CPII for type II collagen synthesis), histopathological changes (Mankin score, based on the toluidine blue staining of proteoglycans), and immunohistochemical staining of matrix metalloproteinase (MMP)-13 and type II collagen, using a rat experimental osteoarthritis (OA) model. Anterior cruciate ligament transection (ACLT) was performed on the right knee joint to surgically induce OA. Animals were divided into four groups: Control group (Control), sham-operated group (Sham), ACLT group without collagen peptide (ACLT group) and ACLT group with oral administration of CP (CP group). ACLT induced histological damages and significantly increased the Mankin score (P<0.05). However, CP administration markedly suppressed the Mankin score, although this difference was not significant. In addition, serum CTX-II levels were significantly decreased in CP group compared with those in the ACLT group (P<0.05). By contrast, serum CPII levels did not differ significantly among the four groups. Moreover, immunohistochemical staining of type II collagen and MMP-13 (an important type II collagen-degrading enzyme) indicated that the amount of type II collagen increased, whereas the number of MMP-13 positive chondrocytes decreased in the CP group compared with ACLT group. These observations suggest that CP has the potential to exert chondroprotective action on OA by inhibiting MMP-13 expression and type II collagen degeneration.

Evaluation of the anti-inflammatory actions of various functional food materials including glucosamine on synovial cells

“…Hyaluronic acid exerts the anti-inflammatory action by inhibiting bradykinin-induced arachidonic acid release from synovial fibroblasts in osteoarthritic inflamed joints (18). Collagen peptides exert a chondroprotective action on OA by inhibiting matrix metalloproteinase-13 expression and type II collagen degeneration (19). Olive leaf extract exhibits the anti-arthritis effect by preventing the destruction of cartilage and limiting peri-articular soft tissue inflammation in kaolin-and carrageenan-induced arthritis (21).…”

“…IL-1β was purchased from Peprotech EC Ltd. (London, UK). In addition, the effects of the following substances, which are known to exhibit anti-inflammatory or anti-arthritic action (17)(18)(19)(20)(21)(22)(23)(24)(25), were evaluated: Proteoglycan extracted from salmon nasal cartilage (17) was supplied by Ichimaru Pharcos Co., Ltd. (Gifu, Japan), hyaluronic acid (18) by N.C. Corporation (Kagawa, Japan), collagen peptides (19,20) by Nitta Gelatin Inc. (Osaka, Japan), olive leaf extract (21) by Eisai Food & Chemical Co., Ltd. (Tokyo, Japan), red ginger extract (22) from Oryza Oil & Fat Chemical Co., Ltd. (Aichi, Japan), Boswellia serrata extract (23) from Nippon Shinyaku Co., Ltd., devil's claw extract (24) from Ask Intercity Co., Ltd. (Chiba, Japan) and curcumin (25) from Indena Japan Co., Ltd. (Tokyo, Japan).…”

Bushenhuoxue Formula Facilitates Articular Cartilage Repair and Attenuates Matrix Degradation by Activation of TGF‐β Signaling Pathway

“…3 μ m sagittal sections were stained with Alcian Blue Hematoxylin/Orange G (ABH) for gross structural analyzing. Mankin scoring system which graded cartilage on a scale of 0-13 was used to evaluate the cartilage lesion [ 25 ]. Higher score means worse repairing.…”

Role of Collagen Derivatives in Osteoarthritis and Cartilage Repair: A Systematic Scoping Review With Evidence Mapping

“…Types of Studies A total of 41 studies have been published on collagen derivatives in OA and cartilage repair so far. Sixteen of these studies were preclinical in vitro and in vivo studies [25,26,[41][42][43][44][45][46][47][48][49][50][51][52][53][54] and 25 were clinical studies (including clinical trials, observational studies, and systematic reviews/ meta-analyses) [27,28,31,. Figure 2 depicts the distribution of the studies by specific study type.…”