Evaluation of the Effect of the Ophthalmic Remedies Combination on the Viability of Human Conjunctival Cell Culture Chang Conjunctiva

Suetina, I. A.; Чернакова, Г. М.; Майчук, Д. Ю.; Russu, L. I.; Клещева, Е. А.; Муртазалиева, С. М.; Мезенцева, М. В.

doi:10.18008/1816-5095-2017-4-368-374

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2017

DOI: 10.18008/1816-5095-2017-4-368-374

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Evaluation of the Effect of the Ophthalmic Remedies Combination on the Viability of Human Conjunctival Cell Culture Chang Conjunctiva

I. A. Suetina¹,

Г. М. Чернакова²,

Д. Ю. Майчук³

et al.

Abstract: цель: изучить цитологический и иммунологический эффекты, оказываемые одновременным воздействием на культуру нормальных клеток конъюнктивы человека Chanq сonjunсtiva офтальмологических препаратов Броксинак® и Офтальмоферон® для совершенствования методики оценки комбинаций офтальмологических лекарственных средств in vitro. материал и методы. Для работы использована перевиваемая культура нормальных клеток конъюнктивы человека Chang conjunctiva, клон 1-5 С-4. Действие препаратов Броксинак® и Офтальмоферон® количес… Show more

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Cited by 2 publications

References 15 publications

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New Properties of the Heparin-Containing Drug in vitro (Potential Antiviral and Anti-Inflammatory Effects)

Чернакова¹,

Майчук²,

Слонимский³

et al. 2018

Ophthalmology in Russia

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Purpose: to study the cytological, immunological and antiviral effects of the HILOPARIN-KOMOD® drug in vitro.Material and methods.We used transplantable cultures of normal cells of the human Chang conjunctiva, and the kidney cells of the Vero monkeys. The cytotoxic effect of the HILOPARIN-KOMOD® was determined by the effect on the cell viability, and by optical density (OP) of the monolayer of the Chang conjunctiva cell culture using the enzyme immunoassay (ELISA) with MTT. The effect of the drug on the functional activity of conjunctival cells was evaluated by the production of cytokines at the level of their in vitro transcription. The antiviral effect of the drug HILOPARIN-KOMOD® was studied on the Vero cell line infected with herpes simplex virus type 1 (HSV-1) and HSV type 2 (HSV-2). The viral activity of HSV-1 and HSV-2 and the antiherpetic effect after the drug was evaluated by polymerase chain reaction (PCR). Results.No significant cytotoxic effect on the metabolism of conjunctival cells of the preparation HILOPARINKOMOD ® in dilutions from 1/2 to 1/2048 was revealed (in comparison with the control). The effect of the drug in dilutions of 1/40 and 1/1000 on the culture of the conjunctival cells resulted in suppression of the production of interferon λ-1 mRNA (IFNλ-1) and IFNλ-2 mRNA compared to the control. In the dilution of preparation 1/1000, the production of mRNA of interleukin-6 (IL-6) was not revealed with simultaneous presence of IL-10 mRNA. In different dilutions of the drug, the number of copies of HSV-1 virus DNA decreased in comparison with the control in all cases, the greatest antiviral effect was achieved in a 1: 2 dilution. The effect of the preparation HILOPARIN-KOMOD® on the culture of cells infected with HSV-2 in dilutions of 1: 2 and 1: 5 led to a decrease in the level of viral replication by 300 and 40 times, respectively.The conclusion.The drug HILOPARIN-KOMOD® has an anti-inflammatory effect that manifests itself in suppressing the synthesis of the mRNA of the acute phase proinflammatory cytokine IL-6 and stimulating the production of anti-inflammatory IL-10 mRNA, without having a cytotoxic effect on conjunctival cells. The proven antiviral effect of the drug makes it possible to recommend the inclusion of HILOPARIN-KOMOD® in the scheme of therapy of patients with viral ophthalmopathology.

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New Properties of the Heparin-Containing Drug in vitro (Potential Antiviral and Anti-Inflammatory Effects)

Чернакова¹,

Майчук²,

Слонимский³

et al. 2018

Ophthalmology in Russia

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Еpidemiological, Etiological and Clinical Aspects of Acute Infectious Conjunctives — at the Crossroads of Ophthalmology and Epidemiology (Clinical and Laboratory Study)

et al. 2019

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The clinical and laboratory picture of acute infectious conjunctivitis has undergone significant changes in the last few years. This circumstance, apparently, is associated with the appearance of a large number of both eye and systemic forms of antibacterial, anti-inflammatory, immunocorrecting drugs and their widespread use, often without establishing an etiological factor of inflammation. The objective of our work was to propose a scheme of rational empirical therapy of acute infectious conjunctivitis, based on the potential of modern ophthalmopharmacology; and to study the etiological spectrum and features of the clinical picture of the above diseases, thereupon correcting the proposed scheme according to the obtained data. Material and methods. A retrospective analysis of over 20,000 outpatient charts of patients with acute inflammation of the conjunctiva and a prospective examination of 275 patients with acute infectious conjunctivitis was carried out. The method of polymerase chain reaction (quantitative and qualitative) was used to identify the genetic material of the pathogen in scrapings from the conjunctiva, the oropharynx and the nasopharynx. Results. The data of the retrospective analysis has shown that when the symptoms indicated acute infectious conjunctivitis, the actual diagnosis of an ophthalmologist was adenoviral conjunctivitis. There were no other nosological forms with the type of pathogen recorded in outpatient charts. The peak incidence was in the winter-spring period, with the highest number of cases in 2011 and 2013 (1509 and 1482 respectively). Results of the prospective study showed the leading role of adenoviruses (20 % of cases), hemophilic rod (18 % of cases) and pneumococcus (12 % of cases) in the development of acute inflammation of the mucous membrane of the eye. As a concomitant infection, the bacteria of the staphylococcus group were found most often. The spectrum of the revealed pathology confirmed the validity of the selected empirical therapy. Conclusion. The results of the study allow us to recommend the prescription of three groups of drugs (antibiotic, antiviral, non-steroidal anti-inflammatory drug) in the treatment of acute infectious conjunctivitis.

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Evaluation of the Effect of the Ophthalmic Remedies Combination on the Viability of Human Conjunctival Cell Culture Chang Conjunctiva

Cited by 2 publications

References 15 publications

New Properties of the Heparin-Containing Drug in vitro (Potential Antiviral and Anti-Inflammatory Effects)

New Properties of the Heparin-Containing Drug in vitro (Potential Antiviral and Anti-Inflammatory Effects)

Еpidemiological, Etiological and Clinical Aspects of Acute Infectious Conjunctives — at the Crossroads of Ophthalmology and Epidemiology (Clinical and Laboratory Study)

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