Evaluation of the Effect of 3 Probiotics on Experimental <i>Escherichia coli</i> Enterotoxaemia in Weaned Piglets*

Cupere, Francoise de; Deprez, Piet; Demeulenaere, D; Muylle, E.

doi:10.1111/j.1439-0450.1992.tb01169.x

Cited by 40 publications

(18 citation statements)

References 14 publications

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“…Administration of lactic acid bacteria has been shown to reduce intestinal coliform and Enterobacteriaceae counts in the majority of previous studies (4,22,24,28,38,39), although some have seen no effects (7,13). The trend towards positive effects observed in ours and other studies, although not significant, should not be ignored, as individual variations in the responses of different animals are to be expected due to the complexity of the intestine (36).…”

Section: Resultscontrasting

confidence: 33%

Relative Ability of Orally Administered Lactobacillus murinus To Predominate and Persist in the Porcine Gastrointestinal Tract

Gardiner¹,

Casey

et al. 2004

Appl Environ Microbiol

101

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Five porcine-derived Lactobacillus or Pediococcus isolates administered to pigs (n ‫؍‬ 4), either singly or as a combination at ϳ10 10 CFU per day varied with respect to intestinal survival and persistence. Two Lactobacillus murinus strains survived best and were excreted at ϳ10 7 to 10 8 CFU/g of feces. In contrast, Pediococcus pentosaceus DPC6006 had the lowest fecal count at ϳ10 5 CFU/g and was excreted at a significantly lower level than both L. murinus strains. Fecal L. murinus DPC6003 counts were also significantly higher than both Lactobacillus salivarius DPC6005 and Lactobacillus pentosus DPC6004 (ϳ10 6 CFU/g). The L. murinus strains persisted for at least 9 days postadministration in both the feces and the cecum. Animals fed a combination of all five strains excreted ϳ10 7 CFU of the administered strains/g, with L. murinus predominating, as determined by randomly amplified polymorphic DNA PCR. Postadministration, variation was observed between animals fed the strain combination, but in general, L. murinus DPC6002 and DPC6003 and L. pentosus DPC6004 predominated in the feces and the cecum while P. pentosaceus DPC6006 was detected only in the cecum. Fifteen days after the start of culture administration, mean fecal Enterobacteriaceae counts were significantly lower in some of the treatment groups. In addition, when mean preadministration counts were compared with those obtained after 21 days of culture administration, Enterobacteriaceae counts were reduced by ϳ87 to 98% in pigs fed L. salivarius DPC6005, P. pentosaceus DPC6006, L. pentosus DPC6004, and the culture mix. In conclusion, the porcine intestinal isolates have potential as probiotic feed additives for pigs, with differences in strain performance highlighting the advantages of using culture combinations.

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Section: Resultscontrasting

confidence: 33%

Relative Ability of Orally Administered Lactobacillus murinus To Predominate and Persist in the Porcine Gastrointestinal Tract

Gardiner¹,

Casey

et al. 2004

Appl Environ Microbiol

101

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“…Challenge studies with piglets and E. coli 0141 :K85 showed no influence on clinical symptoms, mortality or excre tion of haemolytic E. coli (De Cupere et al, 1992). In a study conducted in our institute, it could also be shown that although the colonization with mucosa-asso ciated enterobacteria was reduced by a probiotic B. cereus preparation (Jadamus et , the probiotic had no influence on the occurrence of pathogenic E. coli as measured with a PCR assay (Goebel et al, 2000).…”

Section: Influence On Intestinal Microbial Communitiesmentioning

confidence: 87%

Probiotic feed additives - effectiveness and expected modes of action

Simon¹,

Jadamus²,

Vahjen³

2001

J. Anim. Feed Sci.

127

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In animal nutrition, probiotics are viable microorganisms used as a feed supplement, which lead to beneficial effects for the host animal. For most species a trend towards improved performance has been reported due to the use of probiotics, but statistically significant improvements of weight gain and of feed conversion are rare, mainly because of variations in the individual reactions of the ani mals. However, reductions of the incidence of diarrhoea were significant in most studies with piglets and calves. In contrast to human nutrition, where Lactobacillus species are common probiotic pro ducts, in animal nutrition Enterococcus spp., Saccharomyces yeast, and spore-forming Bacillus spp. are most frequently used as probiotic microorganisms. Furthermore, in human nutrition long-term effects for lasting health promotion and longevity are sought, but the aims of probiotic supplementa tion in modern animal production are fast effects such as improvement of weight gain and feed conversion. Thus, results from probiotic research in the field of human nutrition are not completely valid for animal nutrition.Although 19 probiotic preparations have received temporary approval in the European Union, their modes of action, which lead to beneficial effects, are only partly known. It is very probable that the impact of probiotics on pathogenic and nonpathogenic intestinal bacteria is of prime importance. However, modifications of microstructures and barrier functions of intestinal tissues as well as reac tions of the immune system seem to be involved in the overall effect, directly or as a consequence of modified bacterial populations. In order to specifically improve probiotics and their application in defined indications, more research is needed to determine their underlying mechanisms.

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“…Results are the means of at least three experiments. This decrease in viability may explain the necessity of supplying Bacillus based probiotics in high concentrations and on a daily basis (6). It has been shown that results obtained in vitro highly correlate with those obtained in vivo, thus allowing the use of the formers in the selection of probiotic candidates (10).…”

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confidence: 88%

Properties of the Bacillus Cereus strain used in probiotic CenBiot

et al. 1999

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Bacillus cereus CenBiot fulfilled the requirements to be used as probiotic. The spores showed D 80 of 14 hs, inhibited Escherichia coli and Yersinia pseudotuberculosis after 24 hs in associative culture, were innocuous for suckling and adult mice and were not inhibited by antibiotics at low concentrations. Revista de Microbiologia (1999) Probiotics are "viable mono or mixed cultures of microorganisms which, applied to animal or man, beneficially affect the host by improving the properties of the indigenous flora" (10). They must promote growth, improve feed conversion and inhibit enteropathogens, without causing any undesirable effect. In addition, they must survive the stress produced during manufacturing, storage and administration at farm conditions. Key words: probiotic, Bacillus cereusThe search for new probiotic strains has increased in recent years due to the necessity to find economic and effective substitutes for antibiotics used as feed additives. Several strains of Lactobacillus, Pediococcus, Bacteroides, Bifidobacterium, Bacillus, Streptococcus and Escherichia coli, alone or consortiated, have been used as probiotics (8), giving controversial results. Among the various species of probiotics, those belonging to the genus Bacillus have the advantage that, due to their capacity to sporulate, they survive at ambient temperatures as well as during desiccation by methods that involve moderate heating, such as spray dryers, avoiding the use of lyophilization or other expensive technologies (10). This property also makes possible the administration of spores mixed with powdered vehicles instead of gels or liquids used with non sporulated bacteria. Although several strains of different species of Bacillus are being used for this purpose, information concerning their properties was seldom reported.Probiotic CenBiot, prepared with a strain of Bacillus cereus at Centro de Biotecnologia of Universidade Federal de Pelotas, Rio Grande do Sul, Brazil, was tested in commercial farms showing beneficial effects in the control of diarrhoea and feed conversion in pigs (21). The effects of some factors that could affect the survival of Bacillus cereus in the intestinal tract and during manufacturing, such as interaction with enteropathogens, resistance to heat and to variation of pH, are reported in this work.The strain was classified as Bacillus cereus due to its morphology, Gram staining, capacity to form 12 C. Gil-Turnes et al. thin wall spores in aerobic conditions, growth in glucose broth under anaerobic conditions, production of lecithinase and acetyl-methyl-carbinol and absence of urease (20). To obtain spore suspensions, cultures were heated at 80 o C for 15 minutes, centrifuged, and after three successive washings with ultrapure water, resuspended in ultrapure sterile water pH 7.2.To test the effect of heat, aliquots of 300µl of spore suspensions were heated in a water bath at 50 o C for 4 days, 80 o C for 60 min, 85 o C for 60 min and 90 o C for 40 min. Samples were collected every 24 hours, 10 min,...

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Evaluation of the Effect of 3 Probiotics on Experimental Escherichia coli Enterotoxaemia in Weaned Piglets*

Cited by 40 publications

References 14 publications

Relative Ability of Orally Administered Lactobacillus murinus To Predominate and Persist in the Porcine Gastrointestinal Tract

Relative Ability of Orally Administered Lactobacillus murinus To Predominate and Persist in the Porcine Gastrointestinal Tract

Probiotic feed additives - effectiveness and expected modes of action

Properties of the Bacillus Cereus strain used in probiotic CenBiot

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