Evaluation of the Effect of Titatnium Dioxide Nanoparticles/Gelatin Composite on Infected Skin Wound Healing; An Animal Model Study

Abstract

Objective:To evaluate effects of titanium dioxide /gelatin nanocomposite on wound healing in mice as a model study. Methods:Fifty male rats were randomized into five groups of ten animals each. In group I, 0.1 mL sterile saline 0.9% solution was added to the wounds with no infection. In group II, the wounds were infected with MRSA and only treated with 0.1 mL the sterile saline 0.9% solution. In group III, infected wounds were treated with gelatin. In group IV, animals with infected wounds were treated with 0.1 mL titanium dioxide nanoparticles. In group V, animals with infected wounds were treated with titanium dioxide /gelatin nanocomposite. Wound size was measured on 2, 6, 10, 14, 18 and 20 days after surgery. Results:Reduction in wound area indicated that there was significant difference between group IV and other groups (p<0.05). Quantitative histological and morphometric studies and mean rank of the qualitative studies demonstrated that there was significant difference between group IV and other groups (P<0.05). Conclusion:Titatnium dioxide nanoparticles/gelatin composite offered potential advantages in wound healing acceleration and fibroblast proliferation on early days of healing phases. Acceleration in wound repair could be associated with earlier wound contraction and stability of damaged area by rearrangement of granulation tissue and collagen fibers.

<p>Comparative Study of Antibacterial Effects of Titanium Dioxide Nanoparticles Alone and in Combination with Antibiotics on MDR <em>Pseudomonas aeruginosa</em> Strains</p>

“…47 Recently many studies reported that the wound dressing with TDN enhanced wound healing due to its antimicrobial and cell growth stimulation features. [48][49][50][51][52] In contrast to our findings, Kotlhao et al (2017) reported that TDN had no antibacterial activity against Pseudomonas aeruginosa 53 which may be due to the reduction in porosity of Gram-negative cell wall or existence of efflux pump that causes a decrease in intracellular concentrations of these compounds, resulting in a drastic reduction of biocidal activity. 54 The Effect of TDN and Antibiotics on MDR P. aeruginosa Isolates…”

Natural-Based Biomaterial for Skin Wound Healing (Gelatin vs. Collagen): Expert Review

“…Similarly, native gelatin showed good adhesion to fibroblasts and cell viability from 72 h up to 7 days [ 93 , 94 ]. The in vivo studies done by Hsu et al, (2019) [ 95 ], Nikpasand et al, (2019) [ 96 ], and Jang et al, (2017) [ 97 ] indicated the formation of thick granulation tissues, increased re-epithelization and blood vessel formation with an absence of cytotoxic effects confirmed through CCK-8 assays. For both Col and gelatin scaffolds, the analysed physicochemical properties correlated with those of an ideal scaffold for wound healing as per discussed in the physicochemical section above.…”

Contribuições Do Enfermeiro Na Pesquisa Básica: Modelo De Fixação De Curativo Em Feridas Cutâneas Excisionais De Camundongos

“…Observa-se grande número de estudos desenvolvidos em modelos experimentais 12,13 . No entanto, esses modelos utilizados para avaliação do tratamento de feridas não contemplam, em sua totalidade, os princípios da cobertura ideal.…”