Evaluation of the effect of various nutritional and environmental factors on biosurfactant production by Staphylococcus epidermidis

Abstract

Abstract. Alyousif NA, Al-Tamimi WH, Al-sahib MAA. 2022. Evaluation of the effect of various nutritional and environmental factors on biosurfactant production by Staphylococcus epidermidis. Biodiversitas 23: 3533-3538. Biosurfactants are biological surface-active compounds synthesized mainly by hydrocarbon-utilizing bacteria. The properties of biosurfactants make them promising compounds for the application in various fields. The current study evaluated the effect of various nutritional and environmental factors on biosurfactants produced by Staphylococcus epidermidis. This bacterium, for first time in world was isolated from oilfield reservoir in the current study and identified by 16S rDNA sequencing and considered biosurfactant producer according to screening tests. Several factors were evaluated in the current study to assess the optimal conditions for producing the biosurfactant. The results demonstrated that the best carbon source was olive oil with 2% concentration and glutamic acid was the best nitrogen source with 0.2% concentration. The study demonstrated that the best biosurfactant production was recorded at incubation time of 5 days with a 3% inoculum size. Further study is required to determine the chemical structure of produced biosurfactants and to evaluate the potential application of biosurfactants in various fields, therefore constituting a stimulus for future studies.

Application of Biosurfactants in Medical Sciences

“…Biosurfactants can be classified as glycolipids, lipopeptides, neutral lipids, phospholipids, or fatty acids, based on their chemical structures and microbial origin. The types and quantities of biosurfactants produced by microbes are closely related to their growth environment [ 56 ] and nutritional conditions [ 57 ]. Therefore, exploring the growth environment and nutritional conditions of microorganisms is also a popular research topic for expanding the yield of target biological surfactants [ 58 ].…”

Molecular determination of the microbial diversity associated with vaginitis and testing their sensitivity to selected antimicrobials

“…There is growing evidence that connects enterococci to BV and AV. These bacteria were isolated from different environments, including produced water in oil fields (Alyousif et al 2020;Hamzah et al 2020;Aboud et al 2021;Alshami et al 2022;Alyousif et al 2022). One of the bacteria detected at a frequency of 1(4.2%) was Streptococcus agalactiae strain 149, a significant pathogen in AV and other human diseases.…”

Isolation, screening and molecular identification of bioflocculants–producing bacteria

“…The release of wastewater into water sources without suitable treatment may have harmful impacts on the health of both people and the environment. Water contamination can be caused by agricultural practices, unlawful dumping, landfill leachate leaks, and the discharge of industrial and sewage effluent (Arifin et al 2018;Hassimi et al 2020).…”

“…The bacteria produce bioflocculants under normal conditions in extremely small quantities, therefore it is important to explore methods to increase bioflocculants yield by the bacteria. The optimization of the medium composition and the cultivation conditions including (carbon, nitrogen sources, inoculum size, temperature, initial pH of the medium and cultivation time) that affects bioflocculants yield (Gouveia et al 2019;Abbas et al 2020;Alyousif et al 2022). A bioflocculant-producing bacterium was isolated from the soil and wastewater in the current study.…”