Evaluation of the direct effects of poly(dopamine) on the in vitro response of human osteoblastic cells

Abstract

Functional poly(dopamine) coatings promise to become an efficient strategy to endow biomaterials with enhanced bioactive properties.

Probing polydopamine adhesion to protein and polymer films: microscopic and spectroscopic evaluation

“…There is also similarity to Mei et al's [18] spectrum with a common peak near 1600 cm -1 indicative of aromatic rings. There is further similarity to spectra obtained Iqbal et al [69] for PD-coated nanoparticles and good similarity to the spectrum obtained by Steeves et al [5] for PD with nanoporous titanium. Raman spectra were consistent between scans in different locations for the same samples (data not shown); however, the spectra (Fig.…”

“…This is similar to the process for breath figure array formation-where water droplets condensing as the solvent evaporates act as a template for the polymer as it is cast-but here the pores lack order, likely due to the dry environment in which they were cast. With a similar appearance to the PD previously observed via AFM [5], the image of deposited PD (Fig. 2d) shows a majority of small particles (100-250 nm) with some larger aggregates (350-550 nm) which fall in the range of sizes observed by others [5,27,28].…”

“…With a similar appearance to the PD previously observed via AFM [5], the image of deposited PD (Fig. 2d) shows a majority of small particles (100-250 nm) with some larger aggregates (350-550 nm) which fall in the range of sizes observed by others [5,27,28]. This sample was found to be smoother (R a 9.8 ± 0.5 nm) than reported values from Bi et al [23].…”

“…3). Since it has been found that PD supports cell growth [5], it might be expected that the proteins would experience more adhesion though the opposite appears to be true even when the unadjusted values are considered (Supplementary Information 2) except for mucin. The low adhesion observed with the PD-functionalised probes appears contradictory to the ease that PD can functionalise surfaces, as enabled by the displayed catechol, imine and amine groups [32].…”

“…The mussel adhesive-inspired polydopamine (PD) appears to be an ideal biomaterial coating since it fulfils all these criteria. It exhibits low cytotoxicity [2], antibacterial properties [3,4], promotes cell growth [5][6][7][8], resists corrosion [9], can aid antibiotic release [10,11], is simple to produce with control over film thickness [12] and can be attached to a variety of surfaces including silanes [13], metals [9,14], polymers [7,[15][16][17] dentine [18] and even hair [19].…”