Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos

Wang, Rongchun; Liu, Kechun; Zhang, Yun; Chen, Xiqiang; Wang, Handong

doi:10.3389/fphar.2020.00032

Cited by 16 publications

(7 citation statements)

References 48 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This can be observed on the increased expression of BAX and Apaf-1 in embryos with glucose 3% exposure. Earlier research studies showed that BAX has an important role in apoptosis process due to ROS, and increased BAX expression correlates with increased apoptosis rate [28][29][30][31]. The result of this study is linear to earlier studies which stated that increased Apaf-1 expression can increase apoptosis rate in zebrafish embryo through mitochondria-dependent pathway [32][33][34].…”

Section: Salacca Zalacca Peel Reduces Il-1β and Apoptosis Markers Expression Due To High Glucosesupporting

confidence: 86%

Ethanolic extract of Salacca zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo

Khotimah

Aninditha

et al. 2021

GSC Biol. and Pharm. Sci.

View full text Add to dashboard Cite

Gestational diabetes is a glucose intolerance level first identified during pregnancy. This condition is caused by hormone secretion that hinders insulin action from the placenta which caused insulin resistance that will cause hyperglycemia. This condition will increase oxidative stress, and in consequence increasing inflammation and cell death which can hinder fetal growth and development. Salacca zalacca peel is a food waste that contains antioxidants and anti-inflammatory properties. This research aimed to study the effect of SZ peel ethanolic extract in the expression of IL-1β and cell death marker (BAX and Apaf-1) in glucose 3% induced zebrafish embryo. Glucose metabolism is measured by the level of Phosphoenolpyruvate carboxykinase (PEPCK) at the age of 3 dpf. The expression of PEPCK, IL-1β, Bax, and Apaf-1 is measured using reverse transcriptase PCR (RT-PCR) method. Salacca zalacca peel extract is given in the concentration of 0,1; 0,2; and 0,4 mg/mL. The result of this research proves that PEPCK decreases in the group which was given glucose 3%. Salacca zalacca peel extract significantly decreases the expression of IL-1β and Bax in the concentration of 0,4 mg/mL, and also able to decrease Apaf-1 expression with the result that most closely resemble the negative control in the concentration of 0,4 mg/mL. In conclusion, Salacca zalacca peel extract protects from gestational diabetes condition through the decrease of IL-1β, Bax, and Apaf-1.

show abstract

Section: Salacca Zalacca Peel Reduces Il-1β and Apoptosis Markers Expression Due To High Glucosesupporting

confidence: 86%

Ethanolic extract of Salacca zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo

Khotimah

Aninditha

et al. 2021

GSC Biol. and Pharm. Sci.

View full text Add to dashboard Cite

show abstract

“…The Bcl2 expression involved in the occurrence of apoptosis. Caspase-3 can accelerate the cleavage of several cellular proteins, resulting in the induction of apoptosis . The real-time RT-PCR study revealed that there was no significant difference in PPAR-α, TGF-α, BCL2, and Caspase-3 expression after AgNCs exposure, compared to the control (see Figure S23a in the Supporting Information).…”

Section: Resultsmentioning

confidence: 98%

“…Caspase-3 can accelerate the cleavage of several cellular proteins, resulting in the induction of apoptosis. 65 The real-time RT-PCR study revealed that there was no significant difference in PPAR-α, TGF-α, BCL2, and Caspase-3 expression after AgNCs exposure, compared to the control (see Figure S23a in the Supporting Information). Similarly, TGF-α and BCL2 protein expression also showed no significant change (see Figure S23b in the Supporting Information).…”

Section: ■ Introductionmentioning

confidence: 98%

N-Cholyl d-Penicilamine Micelles Templated Red Light-Emitting Silver Nanoclusters: Fluorometric Sensor for S^2– Ions and Bioimaging Application Using Zebrafish Model

et al. 2022

View full text Add to dashboard Cite

Red-light-emitting silver nanoclusters (AgNCs) are recently emerged as a promising nanoprobe in the field of biomedical applications, because of their attractive properties, including brightness, luminescence stability, and better biocompatibility. In this report, we have developed highly water-soluble redlight-emitting AgNCs by using N-cholyl D-penicilamine (NCPA) as a biosurfactant at above the critical micelle concentration (CMC) at room temperature. Moreover, the NCPA was initially synthesized by demonstrating the reaction between cholic acid and D-penicilamine via a simple coupling reaction strategy. The primary and secondary critical micellar concentration (CMC) of NCPA surfactant was measured using pyrene (1 × 10 −6 M) as a fluorescent probe, and the values were found to be 3.18 and 10.6 mM, respectively. Steady-state fluorescence measurements reveal that the prepared AgNCs shows the excitation and emission maxima at 365 and 672 nm, respectively, with a large Stokes shift (307 nm). The average lifetime measurements and quantum yield of the AgNCs were calculated to be 143.43 ns and 16.34%, respectively. Also, the red luminescent NCPA-templated AgNCs was synthesized in various protic and aprotic polar solvents, among which DMF and DMSO exhibit bright emission at longer wavelength as synthesized in aqueous medium. At higher concentration of AgNO 3 , bright luminescent and highly stable solid AgNCs was obtained with excitation and emission maxima at 607 and 711 nm, respectively. Furthermore, the synthesized AgNCs has been successfully utilized as a fluorescent probe for selective and sensitive detection of S 2− ions at nanomolar level in water samples, showing its potential applicability for the detection of S 2− ions in drinking, river, and tap water samples. Finally, toxicity and bioimaging studies of NCPA-templated AgNCs was demonstrated using zebrafish as in vivo model, showing no significant toxicity up to 200 μL/mL. The AgNCs-stained embryos exhibited red fluorescence with high intensity, which shows that AgNCs are stable in a living system.

show abstract

“…Cebral et al ( 56 ) suggested that H 2 O 2 causes damage to embryos. Wang et al ( 57 ) reported that elevation of ROS promotes the malformation rate of zebrafish embryos. In the present study, exposure to chloroacetamide and its metabolites promoted generation of ROS and increased cell apoptosis in vivo and in vitro .…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity of chloroacetamide herbicides and their metabolites in vitro and in vivo

Zhang

Guan

et al. 2021

Int J Mol Med

View full text Add to dashboard Cite

The toxicity of chloroacetamide herbicide in embryo development remains unclear. Acetochlor (AC) is a chloroacetamide that metabolizes into 2-ethyl-6-methyl-2-chloroacetanilide (CMEPA) and 6-ethyl-o-toluidine (MEA). The present study determined the potential effect of AC and its metabolites on embryo development. Both HepG2 cells and zebrafish embryos were exposed to AC, CMEPA and MEA in the presence or absence of co-treatment with anti-reactive oxygen species (ROS) reagent N-acetylcysteine. The generation of ROS, levels of superoxide dismutase (SOD) and glutathione (GSH) in HepG2 cells and lactate dehydrogenase (LDH) leakage from HepG2 cells were investigated. The effects of AC, CMEPA and MEA on DNA breakage, MAPK/ERK pathway activity, viability and apoptosis of HepG2 cells were examined by comet assay, western blotting, MTT assay and flow cytometry, respectively. Levels of LDH, SOD and GSH in zebrafish embryos exposed to AC, CMEPA and MEA were measured. The hatching and survival rates of zebrafish embryos exposed to AC, CMEPA and MEA, were determined, and apoptosis of hatched fish was investigated using acridine orange staining. The present data showed AC, CMEPA and MEA induced generation of ROS and decreased levels of SOD and GSH in HepG2 cells, which in turn promoted DNA breakage and LDH leakage from cells, ultimately inhibiting cell viability and inducing apoptosis, as well as phosphorylation of JNK and P38. However, co-treatment with N-acetylcysteine alleviated the pro-apoptosis effect of AC and its metabolites. Moreover, exposure to AC, CMEPA and MEA lead to toxicity of zebrafish embryos with decreased SOD and GSH and increased LDH levels and cell apoptosis, ultimately decreasing the hatching and survival rates of zebrafish, all of which was attenuated by treatment with N-acetylcysteine. Therefore, AC and its metabolites (CMEPA and MEA) showed cytotoxicity and embryo development toxicity.

show abstract

Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos

Cited by 16 publications

References 48 publications

Ethanolic extract of Salacca zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo

Ethanolic extract of Salacca zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo

N-Cholyl d-Penicilamine Micelles Templated Red Light-Emitting Silver Nanoclusters: Fluorometric Sensor for S^2– Ions and Bioimaging Application Using Zebrafish Model

Genotoxicity of chloroacetamide herbicides and their metabolites in vitro and in vivo

Contact Info

Product

Resources

About

Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos

Cited by 16 publications

References 48 publications

Ethanolic extract of Salacca zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo

Ethanolic extract of Salacca zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo

N-Cholyl d-Penicilamine Micelles Templated Red Light-Emitting Silver Nanoclusters: Fluorometric Sensor for S2– Ions and Bioimaging Application Using Zebrafish Model

Genotoxicity of chloroacetamide herbicides and their metabolites in vitro and in vivo

Contact Info

Product

Resources

About

N-Cholyl d-Penicilamine Micelles Templated Red Light-Emitting Silver Nanoclusters: Fluorometric Sensor for S^2– Ions and Bioimaging Application Using Zebrafish Model