Evaluation of the Deswelling Period in Dextran-Containing Medium After Corneal Organ Culture

Borderie, Vincent; Baudrimont, M.; Lopez, M. Algara; Carvajal, Santos; Laroche, L.

doi:10.1097/00003226-199703000-00015

Cited by 57 publications

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“…Furthermore, keratocyte density seems to decrease during organ culture . In addition, we found that four days of incubation with dextran-containing organ culture medium induced lysis of some keratocytes (Borderie et al 1997). In the present study, keratocyte injuries found in two corneas with abnormal light microscopic examination consisted of gross cellular edema and internal cytolysis.…”

Section: Discussionsupporting

confidence: 54%

Light microscopic evaluation of human donor corneal stroma during organ culture

Borderie

Martinache

Sabolic

et al. 1998

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To try to facilitate evaluation of corneal stroma during organ culture by means of light microscopy. Methods: Corneal stroma of 53 consecutive organ-cultured corneas was studied by means of light microscopy during endothelial quality control. Out of 9 corneas with bad stromal evaluation, 2 were studied by means of transmission electron microscopy, and 7 were grafted. From the remaining 44 corneas with a normal light microscopic appearance, 35 were grafted. Results: Stromal abnormalities consisted of bright visible structures with a celllike shape corresponding to keratocyte injuries (i.e. cellular edema, light and dark vacuoles, cell membrane disruption and, finally, internal cytolysis) as observed by TEM. At 3 months postoperatively no clinical differences between the two groups of transplants were observed. Conclusion: Corneal stroma can be evaluated qualitatively and easily by means of light microscopy during organ culture. Further studies are needed to investigate whether the presence of lysed keratocytes in the graft's stroma actually influences the outcome of transplantation.

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Section: Discussionsupporting

confidence: 54%

Light microscopic evaluation of human donor corneal stroma during organ culture

Borderie

Martinache

Sabolic

et al. 1998

Acta Ophthalmologica Scandinavica

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“…The preoperative ECC were taken before these culturemedium preserved corneas were put into Dextran to deswell, which has been shown to lead to a loss of endothelial cells in excess of 8%. 10 Consequently, the actual preoperative ECC is likely to be lower than that provided by the eye bank. Despite this, our mean ECL are comparable with recent literature.…”

Section: Discussionmentioning

confidence: 99%

Endothelial cell loss and complication rates with combined Descemets stripping endothelial keratoplasty and cataract surgery in a UK centre

Jones

Fajgenbaum

Hollick

2015

Eye

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Purpose To report 6-month, 1-and 2-year endothelial cell loss (ECL), intra-and postoperative complications in a large series of patients undergoing either Descemets stripping endothelial keratoplasty (DSEK) or a combined phacoemulsification and DSEK in a UK centre. Patients and methods Patients undergoing DSEK with or without concurrent cataract surgery were included in this retrospective study. Surgeries were performed between January 2006 and May 2013. Main outcomes included intra-and postoperative complications and percentage ECL.Results DSEK was performed in 226 eyes (210 patients). Of these, 141 eyes (126 patients) underwent DSEK alone and 85 eyes (84 patients) underwent DSEK combined with cataract surgery. Excluding complex anterior segment pathology the mean percentage ECL at 6, 12 and 24 months was 40.5 ± 13.4, 45.1 ± 14.6 and 53.1 ± 13.0 in the DSEK group and 40.7 ± 15.4, 42.6 ± 15.3 and 49.6 ± 16.5 in patients undergoing the combined procedure, respectively. There was no significant difference in percentage ECL at 6 or 24 months between the two groups both in complex and routine cases. Intraoperative complications occurred in four patients undergoing DSEK and three undergoing combined procedure. Postoperative complication rates did not reach statistical significance between the groups. Conclusion Mean ECL and complication rates were comparable at 6, 12 and 24 months in routine cases undergoing concurrent DSEK with cataract surgery and those undergoing DSEK. These data support the combined procedure in patients requiring both cataract surgery and endothelial keratoplasty. Further collaboration to report endothelial cell counts from other UK centres should be encouraged.

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“…It has been shown that the presence of dextran in the deswelling media may induce endothelial alterations. 31 In addition, decrease of endothelial cell density between 5% and 10% has been found after longer periods of organ culture storage. 32 In the conditions of our experiments, corneas stored for 12 days in Optisol-GS after a 24 h period in organ culture with 15-epi-LXA4 had a decrease in cell damage that ranged from 36% to 66%.…”

Section: Discussionmentioning

confidence: 99%

Aspirin-Triggered Lipoxin A4 (15-epi-LXA4) Increases the Endothelial Viability of Human Corneas Storage in Optisol-GS

Kakazu²,

Bazán³

et al. 2011

Journal of Ocular Pharmacology and Therapeutics

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Purpose: The human corneal endothelium has a very low mitotic rate, and with aging there is a decrease in the number of cells. 15-epi-LXA4 is an anti-inflammatory, bioactive lipid formed when aspirin acetylates cyclooxygenease-2 and redirects cyclooxygenease-2 catalytic activity away from prostaglandins. The purpose of the current study was to evaluate the action of 15-epi-LXA4 in the endothelium viability of human corneas stored in Optisol-GS. Methods: Human corneal endothelial (HCE) cells along with the Descemet's membrane were isolated from fresh human eyes obtained from National Disease Research Interchange. Cell phenotype was identified by using the tight junctions cell marker ZO-1. LXA4 receptor (FPR2/ALX) was detected by immunostaining of HCE cells and human corneal tissue using a polyclonal antibody. Cell proliferation was evaluated with Ki-67 antibody. To measure cell migration, confluent HCE cells were wounded by a linear scraping with a sterile pipette tip in the center of the well and incubated for 24 h with or without 15-epi-LXA4. To evaluate the reparative capacity of 15-epi-LXA4, 7 pairs of human corneas were incubated in Dulbecco's modified Eagle's medium/F12 media at 37°C with or without 100 nM 15-epi-LXA4 for 24 h and then stored at 4°C in Optisol-GS for 12 days. Endothelial viability was assessed by 2 staining techniques: a viability/cytotoxicity kit and trypan blue combined with alizarin red S. Results: HCE cells and the endothelium of human corneal sections strongly expressed the LXA4 receptor. There was a 3-fold increase in cell proliferation when HCE cells were incubated with 100 nM 15-epi-LXA4 for 24 h. No significant migration was observed after 24 h incubation with 15-epi-LXA4. Corneas incubated for 24 h in Dulbecco's modified Eagle's medium/F12 media in the presence of 15-epi-LXA4 and then stored for 12 days in Optisol-GS had a 36% to 56% increase in viability compared with controls without 15-epi-LXA4. Conclusions: 15-epi-LXA4 is an important mediator that protects the integrity of the human endothelium during corneal preservation in Optisol-GS.

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Evaluation of the Deswelling Period in Dextran-Containing Medium After Corneal Organ Culture

Cited by 57 publications

References 0 publications

Light microscopic evaluation of human donor corneal stroma during organ culture

Light microscopic evaluation of human donor corneal stroma during organ culture

Endothelial cell loss and complication rates with combined Descemets stripping endothelial keratoplasty and cataract surgery in a UK centre

Aspirin-Triggered Lipoxin A4 (15-epi-LXA4) Increases the Endothelial Viability of Human Corneas Storage in Optisol-GS

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