Evaluation of the cytotoxicity of different root canal sealers on L929 cell line by MTT assay

Abstract

The purpose of this study was to evaluate the cytotoxic effects of commercially available root canal sealers [Sealite Ultra (SU), Tubli-Seal (TS), Tubli-Seal EWT (TS-EWT), Pulp Canal Sealer (PCS), Pulp Canal Sealer EWT (PCS-EWT), Endomethasone N (En N), and Apexit Plus (AP)] on L929 cells by using MTT assay. After incubation with each sealer's extract at 37°C in a humidified air atmosphere containing 5% CO 2 for 24 h, MTT (5 mg/mL) in saline was added into each well and further incubated at 37°C for 4 h. Formazan precipitate was dissolved in a buffer containing 23% sodium dodecyl sulfate and 50% N,N-dimethylformamide (pH 4.7). Optical densities of dissolved formazan were read using a microplate spectrophotometer. AP, TS, and TS-EWT showed no cytotoxicity at any dilution tested. Other sealers exhibited some degree of cytotoxicity at the 1/4 and 1/2 dilutions. PCS-EWT and SU exerted more potent cytotoxicity at 1/2 dilution than the other sealers.

The effects of Sarconesiopsis magellanica larvae (Diptera: Calliphoridae) excretions and secretions on fibroblasts

“…A 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium (MTT Sigma M2128) bromide solution was used for determining cell viability, following a previously described procedure (Yilmaz et al, 2012), with some modifications. Five thousand cells were seeded per well on a 96-well Polysorb plate.…”

Inhibiting the role of Skp2 suppresses cell proliferation and tumorigenesis of human gastric cancer cells via the upregulation of p27kip1

“…MTT assay. The cell survival rate and proliferation were determined using an MTT assay, as described (26,27). In brief, the BGC-823 cells and MKN-45 cells were plated into 48-well plates at 1,000 cells/well, and the cells were transfected with Skp2-specific shRNA or negative control (N.C.) shRNA using Lipofectamine 2000 for 24, 48, 72 and 96 h, respectively, at room temperature.…”

A Comparative Study on Root Canal Repair Materials: A Cytocompatibility Assessment in L929 and MG63 Cells

“…A number of fibroblast cell lines, including L929 and 3T3, have been widely used for MTT assays due to their availability and reproducible outcomes [8–10]. Since there is direct contact between root canal repair materials and periapical tissues, these materials are expected to exhibit osteoinductive or osteoconductive properties that promote bone deposition and eventually root canal repair [4, 11].…”