Evaluation of the cytotoxicity, genotoxicity and apoptotic induction of an aqueous extract of Achyrocline satureioides (Lam.) DC

Sabini, María Carola; Cariddi, Laura Noelia; Escobar, Franco Matías; Mañas, Fernando; Comini, Laura R.; Reinoso, Elina; Sutil, Sonia; Acosta, A.C.; Montoya, Susana Nuñez; Contigiani, Marta Silvia; Zanon, Silvia; Sabini, Liliana

doi:10.1016/j.fct.2013.08.005

Cited by 28 publications

(23 citation statements)

References 21 publications

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“…Previous studies have reported that extracts from A. satureioides can induce apoptosis in cancer cells. 2,3 Many of these biological properties have been attributed to its phenolic constituents, with two major flavonoids, quercetin and luteolin, identified for their anticancer activity. 2,4 Our group recently reported the isolation of two molecules from A. satureioides, achyrobichalcone (ACB), and 3-O-methylquercetin (3OMQ).…”

Section: Introductionmentioning

confidence: 99%

Achyrocline satureioides compounds, achyrobichalcone and 3‐O‐methylquercetin, induce mitochondrial dysfunction and apoptosis in human breast cancer cell lines

et al. 2020

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Natural products are a valuable source of new molecules and are important for drug discovery. Many chemotherapeutics currently in clinical use were originated from natural sources and are effective cytotoxic agents. In this study, we investigated the cytotoxic and pro‐apoptotic effects of achyrobichalcone (ACB) and 3‐O‐methylquercetin (3OMQ), two novel compounds isolated from the Achyrocline satureioides plant. Because extracts from this plant have been shown to have anticancer activity in vitro, we evaluated ACB and 3OMQ using a human breast cancer cell line, MDA‐MB‐231, and a nontumorigenic human breast epithelial cell line, MCF‐12A. We found that ACB demonstrates cytotoxic effects on MDA‐MB‐231 cells, but not MCF‐12A cells. 3OMQ also demonstrated cytotoxic effects on MDA‐MB‐231 cells, but with lower selectivity compared to treated MCF‐12A cells. Cell death by both compounds was associated with caspase‐9 and caspase‐3/7 activation. Using high‐resolution respirometry, we found that ACB and 3OMQ were able to cause acute mitochondrial dysfunction in MDA‐MB‐231‐treated cells. These results suggest that apoptosis in MDA‐MB‐231 cells is induced through the activation of the mitochondrial‐dependent pathway. Collectively, these findings suggest that ACB is a strong candidate for further anticancer in vivo tests.

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Section: Introductionmentioning

confidence: 99%

Achyrocline satureioides compounds, achyrobichalcone and 3‐O‐methylquercetin, induce mitochondrial dysfunction and apoptosis in human breast cancer cell lines

et al. 2020

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“…Planta nativa no sul do Brasil (Lorenzi & Matos, 2008), seu uso popular é tradicionalmente considerado medicinal a partir dos extratos das flores, folhas e caule, devido a sua ação antiinflamatória (Nunes et al, 2003;Souza;Bassani &, Schapoval, 2007) antiespasmódica, analgésica e sedativa (Oliveira et al, 2001), antioxidante (Desmarchelier et al, 1998;Grassi-Zampieron;Vieira & Siqueira, 2009), imunoestimulante (Puhlmann et al, 1992). Estudos mostraram que o potencial de cito e genotoxicidade de extrações desta planta varia com a concentração de uso (Rivera et al, 2004;Fachinetto et al, 2007;Sabini et al, 2013;Salgueiro et al, 2016). Revisão de literatura científica sobre a planta também pode ser encontrada em Barata et al (2009) e Retta et al (2012.…”

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Disinfectant activity of the crude hydroalcoholic extract of Achyrocline satureioides (Asteraceae) on Candida spp. isolated in problem-situations of bovine mastitis

Avancini

Campos

Valente

et al. 2016

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RESUMO -O fenômeno de resistência apresentada por leveduras aos antimicóticos bem como a necessidade de insumos de higiene para uso em sistemas de criação animal baseados no modelo agroecológico motivaram o desenvolvimento deste trabalho. Os objetivos foram realizar a análise fitoquímica qualitativa e avaliar a ação antimicrobiana/desinfetante do extrato hidroalcoólico bruto [peso:volume de 5g:100 mL (50 mg mL)] das flores de Achyrocline satureioides sobre uma Candida albicans padrão (ATCC 14053) e isoladas de casos de mastite bovina: C. albicans (n = 1) , C. krusei (n = 21) e C. rugosa (n = 9). O teste foi o de suspensão, com as linhagens em três densidades populacionais (DP): 10 5 , 10 4 e 10 3 UFC mL, nos tempos de contato (TC) de 15 e 30 minutos e de 1 até 10 horas. Com resultados da atividade influenciados pelas variáveis DP e TC, todas as leveduras foram inativadas. Como exemplo na mais alta DP: a padrão estava inativada na leitura de 1 h; dos isolados clínicos, o de C. albicans inativado na leitura das 3 h; sete isolados de C. krusei estavam inativados aos 30 min e os outros 14 na leitura de 1 h; sete isolados de C. rugosa estavam inativados na leitura das 4 h, os outros até às 10 h. Os fitoquímicos identificados foram compostos fenólicos, taninos, taninos condensados e flavonóides. As evidências observadas sugerem o potencial do extrato para uso sobre as fontes de infecção em procedimentos de desinfecção ou de antissepsia, ou em formulações que lhe sirvam de veículo.Palavras-Chave: extrato vegetal; Achyrocline satureioides; Candida spp.; produto natural.ABSTRACT -This work was prompted by the phenomenon of yeasts' resistance to antifungals, and the need for sanitation products for use in livestock farming in the agroecological model. The objectives were to undertake a qualitative phytochemical analysis of, and assess the antimicrobial/disinfectant action of, the crude hydroalcoholic extract [weight:volume of 5g:100 mL (50 mg mL)] of flowers of Achyrocline satureioides on standard Candida albicans (ATCC 14053) and isolates of cases of bovine mastitis: C. albicans (n = 1) , C. krusei (n = 21) and C. rugosa (n = 9). The test was that of suspension, the lineages in three population densities (PD): 10 5 , 10 4 and 10 3 CFU mL, at contact times (CT) of 15 and 30 minutes and from 1 up to 10 hours. Activity results were influenced by the PD and CT variables, with all yeasts inactivated. For example, at the highest PD: the standard strain was inactivated in the reading at 1 hr; of the clinical isolates, C. albicans was inactivated in the reading at 3 hrs; seven isolates of C. krusei at 30 minutes and the other 14 at 1 hr; seven isolates of C. rugosa were inactivated in the reading at 4 hrs, with the others inactivated at 10 hrs. Phytochemicals identified were phenolic compounds, tannins, condensed tannins and flavonoids. The evidence observed suggests the extract's potential for use on sources of infection in disinfection/antisepsis procedures, or in formulations which serve as a vehicle.

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“…For this reason, is necessary to study the toxicity of extracts obtained from A. satureioides, mainly on normal cells, in order to evaluate the potential risk for human. In previous studies, we have demonstrated that cold aqueous extract of A. satureioides was not able to induce cytogenotoxic effects at concentrations used popularly [ 11 ]. Regarding the hot aqueous extract, the most used in folkloric medicine, a previous study showed that it was toxic on Vero cells [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

In VitroandIn VivoCytogenotoxic Effects of Hot Aqueous Extract ofAchyrocline satureioides(Lam.) DC.

Cariddi

Sabini

Escobar

et al. 2015

BioMed Research International

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In this work we extend the toxicological studies of hot aqueous extract of A. satureioides (As-HAE) evaluating cytotoxic and apoptotic effects on human peripheral blood mononuclear cells (PBMCs). We also determine genotoxic action of this extract in vivo. In addition, the extract was chemically characterized. Finally, we established a comparison with previous data of cold aqueous extract. The As-HAE induced cytotoxicity on PBMCs determined by trypan blue dye exclusion (CC50 = 653 μg/mL) and MTT (CC50 = 588 μg/mL) assays being more toxic than cold extract. However, As-HAE as well as cold extract did not induce apoptosis measured by Hoechst 33258 staining, TUNEL assay, and DNA fragmentation analysis. The in vivo micronucleus test showed that As-HAE exerted cytogenotoxic effects on bone marrow of mice, contrary to what was observed with cold extract. The chemical study of As-HAE allowed identifying the flavonoids found in cold extract: luteolin, quercetin, and 3-O-methylquercetin, but at higher concentrations. We suggest that toxic effects induced by As-HAE could be due to high concentrations of these flavonoids. Given that As-HAE is the most used in folkloric medicine, its administration should be controlled in order to prevent potential cell damage.

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Evaluation of the cytotoxicity, genotoxicity and apoptotic induction of an aqueous extract of Achyrocline satureioides (Lam.) DC

Cited by 28 publications

References 21 publications

Achyrocline satureioides compounds, achyrobichalcone and 3‐O‐methylquercetin, induce mitochondrial dysfunction and apoptosis in human breast cancer cell lines

Achyrocline satureioides compounds, achyrobichalcone and 3‐O‐methylquercetin, induce mitochondrial dysfunction and apoptosis in human breast cancer cell lines

Disinfectant activity of the crude hydroalcoholic extract of Achyrocline satureioides (Asteraceae) on Candida spp. isolated in problem-situations of bovine mastitis

In VitroandIn VivoCytogenotoxic Effects of Hot Aqueous Extract ofAchyrocline satureioides(Lam.) DC.

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