Evaluation of the Cytotoxic Potential of Adhesives, with Two on the Market: Scotchbond Universal and Optibond Solo Plus, and an Adhesive in the Experimental Phase: T1

Abstract

In vitro studies evaluating the cytotoxic potential of substances released from dental adhesives are lacking. The purpose of this study was to compare the cytotoxicity of the extracts of dental adhesives Scotchbond Universal and Optibond Solo Plus, and an adhesive in the experimental phase: T1. 3T3 mouse fibroblast cells and MG-63 osteoblast-like cells from human osteosarcoma were exposed for 24 h to serial extract dilutions. Cytotoxicity was determined using an MTT assay. For both cell lines, the cytotoxicity order obtained, of the unfiltered adhesive extracts, was T1 (less cytotoxic) < Optibond Solo Plus < Scotchbond Universal (most cytotoxic).

The Cytotoxicity of OptiBond Solo Plus and Its Effect on Sulfur Enzymes Expression in Human Fibroblast Cell Line Hs27

“…However, in our study, the concentrations higher than 0.3% were so potent that they left no living cells in the culture medium to investigate. In the paper by Santos, OptiBond Solo Plus was described as the most toxic toward the 3T3 fibroblast cell line and as such, is still one that needs further investigation [18]. In comparison, OptiBond Universal, but in the polymerized, safer form, was tested in an in vitro cellular experimental model-monocyte/macrophage cell line SC-where the cytotoxicity was measured by means of the XTT assay [25].…”

“…The crystal violet cell staining method described in [18] was used to evaluate the number of cells. For the crystal violet test, cells were cultured in 96-and 24-well plates for the 24 h-and 48 h-incubations with OptiBond Solo Plus at 37 • C. The medium was removed, cells washed with PBS, and fixed with 200 µL 96% ethanol for 15 min.…”