Evaluation of the clastogenicity and anticlastogenicity of the carotenoid bixin in human lymphocyte cultures

Antunes, Lusânia Maria Greggi; Pascoal, Lívia Mendonça; Bianchi, María de Lourdes Pires; Dias, Francisca L.

doi:10.1016/j.mrgentox.2005.04.006

Cited by 43 publications

(16 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The basal frequency of chromosomal aberrations evaluated in the cultured blood lymphocytes from healthy controls was in line with the results obtained by Karahalil et al [16] in healthy non-smokers. Treatment with 0.5 μg/ml of cisplatin was not cytotoxic to the control cultures, as expected from previous studies [17], but a significant decrease was detected in SCD patients. Cell cycle control is a fundamental process to regulate cell proliferation and mitosis.…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Sensitivity to cisplatin-induced mutations and elevated chromosomal aberrations in lymphocytes from sickle cell disease patients

et al. 2008

View full text Add to dashboard Cite

Sickle cell disease (SCD) is an inherited disorder caused by a single nucleotide substitution in the beta-globin gene. The clinical heterogeneity observed in SCD patients has been attributed to environmental and genetic factors. The patients are subjected to increased oxidative stress, particularly during vaso-occlusive crises and acute chest pain. Another possible cause of oxidative stress in SCD is the high concentration of iron in the patients' plasma. The increase in oxidative stress could be a relevant risk factor for mutagenesis and carcinogenesis. Studies on the frequency of basal chromosomal aberrations in cultured lymphocytes from SCD patients have not been reported so far. In order to contribute to the understanding of the role of the different biomarkers and their relationship with the extremely variable clinical manifestation of SCD, we investigated the frequency of chromosome damage in peripheral lymphocytes from sickle cells patients and healthy controls. We found an increased frequency of chromosome damage and percentage of aberrant metaphases in these patients when compared with control subjects, even at basal values (p<0.05). In the cytogenetic sensitivity assay, the results showed that these patients presented a marked decrease in the mitotic index values compared with healthy controls. Cisplatin-induced chromosomal damage in lymphocytes from these patients was significantly higher than the frequency measured in healthy controls. The results obtained in the present study showed that more investigations are needed in order to elucidate the susceptibility to genomic instability of SCD patients.

show abstract

Section: Discussionsupporting

confidence: 87%

“…We hypothesise that cisplatininduced sensitisation may occur through reactive oxygen species. Cisplatin is a potent genotoxic agent, inducing sister-chromatid exchanges, chromosomal aberrations and micronucleus formation in cultured human lymphocytes [17,22].…”

Section: Discussionmentioning

confidence: 99%

Sensitivity to cisplatin-induced mutations and elevated chromosomal aberrations in lymphocytes from sickle cell disease patients

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The great scavenging capacity of bixin, found in this in vitro study, can explain the in vivo effects of bixin reported in the literature, supporting that it may have future clinical application. The carotenoid bixin was shown to be capable of acting as an antioxidant by intercepting free radicals generated by the commonly used chemotherapeutic drug, clastogen cisplatin, such as superoxide radical and hydroxyl radical (Antunes, Pascoal, Bianchi, & Dias, 2005), and by inhibiting the ROS generation by DNA-cisplatin interaction in a concentration-dependent manner (Rios, Antunes, & Bianchi, 2009). Bixin also reduced the total number of chromosome aberrations, inhibited the increase in lipid peroxidation, and inhibited renal glutathione depletion induced by cisplatin (Silva, Antunes, & Bianchi, 2001).…”

Section: Scavenging Of Ros and Rns By Annatto Extractsmentioning

confidence: 99%

In vitro scavenging capacity of annatto seed extracts against reactive oxygen and nitrogen species

et al. 2011

View full text Add to dashboard Cite

Bixa orellana L. (annatto), from Bixaceae family, is a native plant of tropical America, which accumulates several carotenoids (including bixin and norbixin), terpenoids, tocotrienols and flavonoids with potential antioxidant activity. In the present study, the in vitro scavenging capacity of annatto seed extracts against reactive oxygen species (ROS) and reactive nitrogen species (RNS) was evaluated and compared to the bixin standard. Annatto extracts were obtained using solvents with different polarities and their phenolic compounds and bixin levels were determined by high performance liquid chromatography coupled to diode array detector. All annatto extracts were able to scavenge all the reactive species tested at the low μg/mL range, with the exception of superoxide radical. The ethanol:ethyl acetate and ethyl acetate extracts of annatto seeds, which presented the highest levels of hypolaetin and bixin, respectively, were the extracts with the highest antioxidant capacity, although bixin standard presented the lowest IC(50) values.

show abstract

“…The seed extracts have been reported to exhibit chemopreventive (Agner et al, 2005) and antioxidant activity (Martinez-Tome et al, 2001). Bixin has also been found to have anticlastogenic activity (Antunes et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Preliminary pharmacological screening of Bixa orellana L. leaves

Shilpi

Taufiq-ur-Rahman

Uddin

et al. 2006

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Evaluation of the clastogenicity and anticlastogenicity of the carotenoid bixin in human lymphocyte cultures

Cited by 43 publications

References 31 publications

Sensitivity to cisplatin-induced mutations and elevated chromosomal aberrations in lymphocytes from sickle cell disease patients

Sensitivity to cisplatin-induced mutations and elevated chromosomal aberrations in lymphocytes from sickle cell disease patients

In vitro scavenging capacity of annatto seed extracts against reactive oxygen and nitrogen species

Preliminary pharmacological screening of Bixa orellana L. leaves

Contact Info

Product

Resources

About