Evaluation of the cellmatics and direct monoclonal fluorescence antibody staining for detection of genital chlamydial infections

Peterson, Ellena M.; Alexander, Richard; Aarnaes, Sandra L.; King, S D; Greenwood, J.; Maza, Luis M. de la

doi:10.1016/0732-8893(87)90098-8

Diagnostic Microbiology and Infectious Disease

1987

DOI: 10.1016/0732-8893(87)90098-8

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Evaluation of the cellmatics and direct monoclonal fluorescence antibody staining for detection of genital chlamydial infections

Ellena M. Peterson¹,

Richard Alexander²,

Sandra L. Aarnaes³

et al.

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1989

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“…Therefore, in the past decade more rapid tests that use immunologic detection or DNA probe technology that does not rely on growth of C. trachomatis have been developed. Two of these methods, direct fluorescent-antibody staining (DFA) and enzyme-linked immunosorbent assay (ELISA), because of their commercial availability, have been widely compared with a reference culture (3,5,9,12,13). In the majority of studies that used an optimal culture system, neither assay was as sensitive as culture.…”

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Molecular techniques for the detection of Chlamydia trachomatis

et al. 1989

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San Diego, Calif.) was compared with a culture reference method for the detection of Chlamydia trachomatis. Using stock isolates of each of the 15 serovars (A to K, Ba, Lt, L2, and L3) of C. trachomatis, the lower limit of sensitivity for the DNA probe ranged between 1,086 inclusion-forming units (IFU) for serovar E (Bour) to 2,930 IFU for serovar Lt (440), with the only exception being serovar C (TW-3), with which 99 IFU was detected. There was no cross-reactivity with Chlamydia psittaci (Texas turkey) and Chlamydia pneumoniae (TWAR-183). Bacterial and fungal isolates representing 14 species of normal vaginal fora as well as Neisseria gonorrhoeae gave negative results with the DNA probe when tested at a level of 1.5 x 107 CFU/mI. In addition, the DNA probe, a direct fluorescent-antibody stain (DFA) (MicroTrak; Syva Corp., Palo Alto, Calif.), and an enzyme-linked immunosorbent assay (Chlamydiazyme; Abbott Laboratories, North Chicago, 111.) were compared with culture for the detection of C. trachomatis, using 196 clinical cervical samples. Of the 196 samples, 20 (10%) were culture positive. Of the 176 culture-negative samples, 1 was not evaluated by DNA probe and 4, because of a lack of cellular material, were not evaluated by DFA. The sensitivities of the DNA probe, DFA, and enzyme-linked immunosorbent assay were 60, 75, and 85%, respectively, and specificities were 95, 99, and 97%, respectively. Of the false-positive direct results, there was only one specimen with which more than one direct method was positive, and with this specimen all three direct methods were positive. The majority of false-negative results by the direct methods were from specimens which by the culture method gave <100 IFU per culture.

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Molecular techniques for the detection of Chlamydia trachomatis

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Evaluation of the cellmatics and direct monoclonal fluorescence antibody staining for detection of genital chlamydial infections

Cited by 1 publication

References 9 publications

Molecular techniques for the detection of Chlamydia trachomatis

Molecular techniques for the detection of Chlamydia trachomatis

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