Evaluation of the card agglutination test (CATT/T. evansi) for detection of Trypanosoma evansi infection in water buffaloes (Bubalus bubalis) in Egypt

Hilali, M.; Abdel-Gawad, Ahmed H.; Nassar, Ahmed; Abdel-Wahab, A.; Magnus, E; Büscher, Philippe

doi:10.1016/j.vetpar.2004.02.009

Cited by 25 publications

(23 citation statements)

References 12 publications

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“…evansi could on the other hand also overestimate the prevalence of the disease, as both animals with active infection and animals recovered from infection could be seroconvert. This finding is in agreement with observation of Hilali et al (2004) reporting 24% prevalence of T. evansi infection in water buffaloes in Egypt while none of them were parasitologically positive. Ngaira et al (2005) indicated in camels that there were PCR positive cases missed by CATT/T.…”

Section: Discussionsupporting

confidence: 93%

Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses

Gari

Ashenafi

Alemu

et al. 2010

Trop Anim Health Prod

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Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6%, 36.7%, and 47.6% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6% in CATT/Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from dourine suspected horses were demonstrated in 4.6% of the animals using Woo test. The findings of the present study disclosed that dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference (P>0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference (P<0.05) in the prevalence of the disease between emaciated and animals with good body condition. Assessment of the degree of agreement of the diagnostic tests employed revealed low to fair (k = 0·1 - 0·4) with significantly higher sensitivity by PCR than other tests.

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Section: Discussionsupporting

confidence: 93%

Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses

Gari

Ashenafi

Alemu

et al. 2010

Trop Anim Health Prod

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“…Trypanosomiasis is the most important and serious pathogenic protozoal disease of camel caused by T. evansi infecting a wide range of animals throughout tropical and sub-tropical regions of the world [5][6][7][8]. Theileriosis is an important hemoparasitic disease of animals inducing a variety of clinical manifestations ranging from a subclinical presentation to a fatal disease depending, in part, on the animal species, host, age and the species of the microorganism.…”

Section: Introductionmentioning

confidence: 99%

Blood Parasites in Camels (Camelus dromedarius) in Northern West Coast of Egypt

El-Naga¹,

Barghash²

2016

J Bacteriol Parasitol

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“…This test is sensitive compared to microscopic parasitological methods and can detect early and late antibodies to T. evansi (Verloo et al, 2000). Similarly Hilali et al (2004) was able to detect anti-T. evansi antibodies in water buffaloes experimentally infected with strain from dromedary camel during undetectable parasitemia. The seropositivity in the present study ranged from very strong to moderate agglutination with the majority of the animals showed moderate level but the titer remains at a detectable level.…”

Section: Discussionmentioning

confidence: 95%

Prevalence of Trypanosoma evansi in Dromedary Camels (Camelus dromedarius) in Al-Ahsa Area of Eastern Saudi Arabia

Al-Salameen¹

2020

SJKFU

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The present study investigates the prevalence of Trypanosoma evansi in dromedary camels in Al-Ahsa area of Eastern Saudi Arabia using both parasitological and serological methods. Six hundred camels were examined in this study at three different locations in Al-Ahsa area namely University Veterinary Clinic, Hufof Veterinary Clinic, and Hufof Slaughterhouse. These camels were grouped according to their age into three groups, under 1 to 2 years, over 2 to 9 years and over 9 years old. The association of animal age and sex with Trypanosoma evansi infection was also investigated. Clinical examination of the surveyed camels showed loss of appetite in 2.3% of these animals, 13.3% of the camels suffering from diarrhea, 5.2% showed loss of weight while the rest of the surveyed camels (79.2%) were apparently healthy. Parasitological examination including Wet Mount Technique (WMT), Stained Blood Smears (SBS), and Hematocrit Centrifugation Technique (HCT) of blood samples obtained from the 600 surveyed camels failed to reveal any trypanosomes in these animals. Card Agglutination Test for Trypanosomes (CATT), however, was able to detect the presence of anti-T. evansi antibodies in the serum of 12.17% of the surveyed animals. Most of the seropositive camels were in the age group over 2 to 9 years, followed by those aging over 9 years, while the least seropositive cases were those aging under 1-2 years. The majority of the seropositive camels showed moderate agglutination level. The sex of camels showed high seropositivity to T. evansi in females compared to male camels.

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Evaluation of the card agglutination test (CATT/T. evansi) for detection of Trypanosoma evansi infection in water buffaloes (Bubalus bubalis) in Egypt

Cited by 25 publications

References 12 publications

Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses

Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses

Blood Parasites in Camels (Camelus dromedarius) in Northern West Coast of Egypt

Prevalence of Trypanosoma evansi in Dromedary Camels (Camelus dromedarius) in Al-Ahsa Area of Eastern Saudi Arabia

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