Evaluation of the butyrylcholinesterase expression and activity in CHO, HEK-293 and vero cell lines transformed by dual promoter expression vector

Mirzaie, Vida; Eslaminejad, Touba; Babaei, Homayoon; Nematollahi‐Mahani, Seyed Noureddin

doi:10.3233/jcb-210042

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…After 24h of incubation, the medium was changed with 100 µl of CUR and CC-CUR nanocomposite in serum-free medium without antibiotics. RNA extraction was done according to a published protocol (23).…”

Section: Transfection Of Cellsmentioning

confidence: 99%

Evaluating the effects of Curcumin Nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF- 7, MDA-MB-231 and SKBR3 cell lines

Eslaminejad,

Nematollahi-Mahani,

Sargazi

et al. 2024

Preprint

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Breast cancer is one of the most common diseases worldwide. miR-221 and miR-222 are two microRNAs with pivotal roles in many cellular processes which regulate the Wnt/β-catenin pathway. Curcumin (CUR), a yellow polyphenolic compound, targets numerous pathways relevant to cancer therapy. The main aim of this study was to compare the ability of chitosan curcumin nanoparticle (CC-CUR) with the curcumin in modulating miR-221 and miR-222 expression through Wnt/β-catenin pathway in MCF-7, MDA-MB-231 and SK-BR-3 breast cancer cells. Chitosan-cyclodextrin-tripolyphosphate containing curcumin nanoparticles (CC-CUR) were prepared. Experimental groups including CC-CUR, CUR and negative control were designed. The expression of miR-221 and miR-222 and Wnt/β-catenin pathway genes was measured. The level of miR-221 and miR-222 and β-catenin genes decreased in MCF-7 and MDA-MB-231 cells and WIF1 gene increased in all cells in CC-CUR group. However, in SK-BR-3 cells miRs and WIF1 gene expressions were increased following CC-CUR administration and β-catenin decreased by administration of CUR. Significant decreasing of β-catenin and increasing of WIF1 gene in almost all three cell lines, indicates that this formulation exerts its effect mainly through the Wnt/β-catenin pathway. These preliminary findings may pave the way for the use of curcumin nanoparticles in the treatment of some known cancers.

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Section: Transfection Of Cellsmentioning

confidence: 99%

Evaluating the effects of Curcumin Nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF- 7, MDA-MB-231 and SKBR3 cell lines

Eslaminejad,

Nematollahi-Mahani,

Sargazi

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…After 24 h of incubation, the medium was changed with 100 μl of CUR and CC-CUR nanocomposite in serum-free medium without antibiotics. RNA extraction was done according to a published protocol [23].…”

Section: Transfection Of Cellsmentioning

confidence: 99%

Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines

Eslaminejad,

Nematollahi-Mahani,

Sargazi

et al. 2024

Diagn Pathol

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Background Breast cancer is one of the most common diseases worldwide that affects women of reproductive age. miR-221 and miR-222 are two highly homogeneous microRNAs that play pivotal roles in many cellular processes and regulate the Wnt/β-catenin signaling pathway. Curcumin (CUR), a yellow polyphenolic compound, targets numerous signaling pathways relevant to cancer therapy. The main aim of this study was to compare the ability of chitosan curcumin nanoparticle (CC-CUR) formulation with the curcumin in modulating miR-221 and miR-222 expression through Wnt/β-catenin signaling pathway in MCF-7, MDA-MB-231 and SK-BR-3 breast cancer cell lines. Method Chitosan-cyclodextrin-tripolyphosphate containing curcumin nanoparticles (CC-CUR) were prepared. Cytotoxicity of the CUR and CC-CUR was evaluated. Experimental groups including CC-CUR, CUR and negative control were designed. The expression of miR-221 and miR-222 and Wnt/β-catenin pathway genes was measured. Results The level of miR-221 and miR-222 and β-catenin genes decreased in MCF-7 and MDA-MB-231 cells and WIF1 gene increased in all cells in CC-CUR group. However, the results in SK-BR-3 cell line were unexpected; since miRs and WIF1 gene expressions were increased following CC-CUR administration and β-catenin decreased by administration of CUR. Conclusion Although the composite form of curcumin decreased the expression of miR-221 and miR-222 in MCF-7 and MDA cells, with significant decreasing of β-catenin and increasing of WIF1 gene in almost all three cell lines, we can conclude than this formulation exerts its effect mainly through the Wnt/β-catenin pathway. These preliminary findings may pave the way for the use of curcumin nanoparticles in the treatment of some known cancers.

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Evaluation of the butyrylcholinesterase expression and activity in CHO, HEK-293 and vero cell lines transformed by dual promoter expression vector

Cited by 2 publications

References 34 publications

Evaluating the effects of Curcumin Nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF- 7, MDA-MB-231 and SKBR3 cell lines

Evaluating the effects of Curcumin Nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF- 7, MDA-MB-231 and SKBR3 cell lines

Evaluating the effects of curcumin nano-chitosan on miR-221 and miR-222 expression and Wnt/β-catenin pathways in MCF-7, MDA-MB-231 and SKBR3 cell lines

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