Evaluation of the biological responses of silver nanoparticles synthesized using <i>Pelargonium</i> x <i>hortorum</i> extract

Lopez-Ayuso, Christian Andrea; Garcia-Contreras, Rene; Manisekaran, Ravichandran; Figueroa, Mario; Arenas-Arrocena, Ma. Concepción; Hernandez-Padron, Genoveva; Pozos-Guillén, Amaury; Acosta-Torres, Laura Susana

doi:10.1039/d3ra00201b

Cited by 1 publication

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“…Later, using a sterile 1 mL pipet tip (8 mm), the well was filled with 100 μL of NPs of various concentrations (5, 10, 25, and 50 μg) along with standard 2% chlorhexidine (CHx-25 μg, FGM, Mexico) and distilled water as a control. The plates were incubated at 37 °C for 24 h. The zone of inhibition (ZOI) was measured using a Vernier caliper, and photographs were taken. − In the case of sutures, the exact procedure mentioned above was carried out, but instead of a well, 5 cm sutures were placed in the agar plates using sterile tweezers and incubated for 24 h, after which the ZOI was measured.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and Cytotoxic Effect of Positively Charged Nanosilver-Coated Silk Sutures

Monroy Caltzonci,

Rasu Chettiar,

Ibarra

et al. 2024

ACS Omega

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Sutures are a crucial component of surgical procedures, serving to close and stabilize wound margins to promote healing. However, microbial contamination of sutures can increase the risk of surgical site infections (SSI) due to colonization by pathogens. This study aimed to tackle SSI by synthesizing positively charged silver nanoparticles (P-AgNPs) and using them to produce antimicrobial sutures. The P-AgNPs were reduced and stabilized using polyethylenimine (PEI), a cationic branched polymer. The physiochemical characteristics of P-AgNPs were confirmed from the surface plasmon resonance (SPR) peak at 419 nm, spherical morphology with a particle size range of 8−10 nm, PEI functional groups on NPs, a hydrodynamic diameter of 12.3 ± 2.4 nm, and a zeta potential of 31.3 ± 6 mV. Subsequently, the surfaces of silk sutures were impregnated with P-AgNPs at different time intervals (24, 48, and 96 h) using an ex situ method. Scanning electron microscopy (SEM) and tensile strength studies were conducted to determine the coating and durability of the NP-coated sutures. The NPs were quantified on sutures using inductively coupled plasma optical emission spectrophotometry (ICP-OES), which was in the range of 1−5 μg. Primarily, antimicrobial activity was studied using three microorganisms (Candida albicans, Streptococcus mutans, and Staphylococcus aureus) for both P-AgNPs and suture-coated P-AgNPs using the agar diffusion method. The results showed that only the NPs and NP-coated sutures exhibited enhanced antimicrobial effects against bacteria and fungi. Finally, the cytotoxicity of the sutures was investigated using stem cells from the apical papilla (SCAPs) for 24 h, which exhibited more than 75% cell viability. Overall, the results indicate that NP-coated sutures can potentially be used as antimicrobial sutures to diminish or inhibit SSI in postoperative or general surgery patients.

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Section: Methodsmentioning

confidence: 99%

Antimicrobial and Cytotoxic Effect of Positively Charged Nanosilver-Coated Silk Sutures

Monroy Caltzonci,

Rasu Chettiar,

Ibarra

et al. 2024

ACS Omega

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Evaluation of the biological responses of silver nanoparticles synthesized using Pelargonium x hortorum extract

Abstract: Silver nanoparticle synthesis mediated using Pelargonium x hortorum and its biological assays.

Cited by 1 publication

References 83 publications

Antimicrobial and Cytotoxic Effect of Positively Charged Nanosilver-Coated Silk Sutures

Antimicrobial and Cytotoxic Effect of Positively Charged Nanosilver-Coated Silk Sutures

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