Evaluation of the biological influence of a stable carbon nanohorn dispersion

Horie, Masanori; Komaba, Lilian Kaede; Fukui, Hitoshi; Kato, Haruhisa; Endoh, Shigehisa; Nakamura, Ayako; Miyauchi, Arisa; Maru, Junko; Miyako, Eijiro; Fujita, Katsuhide; Hagihara, Yoshihisa; Yoshida, Yasukazu; Iwahashi, Hitoshi

doi:10.1016/j.carbon.2012.11.015

Cited by 16 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, many researchers have reported that cytotoxicity of CNHs was very low. 6,6,29,35,36 However, a high uptake level of CNHs in RAW 264.7, a well-known murine macrophage cell line, seemed to generate reactive oxygen species (ROS), lysosomal membrane destabilization, cell apoptosis and necrosis. 35 Russier et al reported that human macrophages appeared less responsive to carbon nanomaterials in comparison to murine macrophage.…”

Section: Discussionmentioning

confidence: 99%

Carbon nanohorns allow acceleration of osteoblast differentiationviamacrophage activation

et al. 2016

Self Cite

View full text Add to dashboard Cite

Carbon nanohorns (CNHs), formed by a rolled graphene structure and terminating in a cone, are promising nanomaterials for the development of a variety of biological applications. Here we demonstrate that alkaline phosphatase activity is dramatically increased by coculture of human monocyte derived macrophages (hMDMs) and human mesenchymal stem cells (hMSCs) in the presence of CNHs. CNHs were mainly localized in the lysosome of macrophages more than in hMSCs during coculturing. At the same time, the amount of Oncostatin M (OSM) in the supernatant was also increased during incubation with CNHs. Oncostatin M (OSM) from activated macrophage has been reported to induce osteoblast differentiation and matrix mineralization through STAT3. These results suggest that the macrophages engulfed CNHs and accelerated the differentiation of mesenchymal stem cells into the osteoblast via OSM release. We expect that the proof-of-concept on the osteoblast differentiation capacity by CNHs will allow future studies focused on CNHs as ideal therapeutic materials for bone regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Carbon nanohorns allow acceleration of osteoblast differentiationviamacrophage activation

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…362, 363 Lastly, recent carbon nanohorn (CNH, Figure 1d) studies highlight the counterintuitive nature of higher-order carbon nanomaterial effects in biology. No distinct changes in intracellular ROS levels or cellular proliferation have been noted after treatment with broad-range concentrations of as-produced or albumin-dispersed CNH, 364, 365 even though these allotropes have semiconductor properties. Interestingly, CNH photosensitization becomes apparent when CNH are pre-oxidized, loaded with photoactive dyes, albumin dispersed, and illuminated.…”

Section: Higher Order Carbon Nanomaterialsmentioning

confidence: 93%

Redox-active nanomaterials for nanomedicine applications

et al. 2017

View full text Add to dashboard Cite

Nanomedicine utilizes the remarkable properties of nanomaterials for the diagnosis, treatment, and prevention of disease. Many of these nanomaterials have been shown to have robust antioxidative properties, potentially functioning as strong scavengers of reactive oxygen species. Conversely, several nanomaterials have also been shown to promote the generation of reactive oxygen species, which may precipitate the onset of oxidative stress, a state that is thought to contribute to the development of a variety of adverse conditions. As such, the impacts of NMs on biological entities are often associated with and influenced by their specific redox properties. In this review, we overview several classes of nanomaterials that have been or projected to be used across a wide range of biomedical applications, with discussion focusing on their unique redox properties. Amongst the nanomaterials examined include iron, cerium, and titanium metal oxide nanoparticles, gold, silver, and selenium nanoparticles, and various nanoscale carbon allotropes such as graphene, carbon nanotubes, fullerenes, and their derivatives/variations. Principal topics of discussion include the chemical mechanisms by which the nanomaterials directly interact with biological entities and the biological cascades that are thus indirectly impacted. Selected case studies highlighting the redox properties of nanomaterials and how they affect biological responses are used to exemplify the biologically-relevant redox mechanisms for each of the described nanomaterials.

show abstract

“…18 The low toxicity revealed by in vivo and in vitro toxicological studies is linked to their metal-free synthesis. [19][20][21] The suitability of CNHs for in vivo studies has been further demonstrated by biodistribution studies which showed no obvious toxicologic lesions during the experiments. 22,23 Examples of CNH delivery systems include delivery of anticancer agents, 24 controlled release of drugs, 25 and phthalocyanine hybrids for dual-modality photothermal and photodynamic therapy.…”

Section: Introductionmentioning

confidence: 95%

Multimodal Microscopy Distinguishes Extracellular Aggregation and Cellular Uptake of Single‐Walled Carbon Nanohorns

Devereux

Cheung²,

Daly³

et al. 2018

Chemistry A European J

View full text Add to dashboard Cite

The low toxicity, high surface area, and ease of functionalisation of carbon nanohorns (CNH) makes them attractive systems for cellular imaging, diagnostics and therapeutics. However, challenges remain for the biomedical translation of these and other nanomaterials. A significant task is tuning the surface chemistry to achieve optimal cellular interactions. Herein, we combine real-time fluorescent imaging of nanoparticle cellular uptake and real-time differential interference contrast (DIC) imaging of extracellular media to monitor a) nanoparticle/nanoparticle and b) nanoparticle/cell interactions for CNHs covalently modified with an OFF/ON near-IR dye, the fluorescence of which is switched OFF in extracellular environments and triggered upon cellular internalisation. CHN samples modified with different loadings of the hydrophobic dye are taken as a simple model of drug-loaded nanoparticle systems. The punctate fluorescence suggests the CNHs are delivered to lysosomes and other vesicles of the endocytic pathway. DIC imaging highlights the competition that exists for many particle types, between extracellular aggregation and cellular internalization, the efficiency of which would be dependent upon the amount of fluorophore loading. The results of this study illustrate how complementary real-time imaging methods together with physicochemical characterisation can be used to address the challenges involved in optimising nanoparticle/cell interactions for biomedical applications.

show abstract

Evaluation of the biological influence of a stable carbon nanohorn dispersion

Cited by 16 publications

References 30 publications

Carbon nanohorns allow acceleration of osteoblast differentiationviamacrophage activation

Carbon nanohorns allow acceleration of osteoblast differentiationviamacrophage activation

Redox-active nanomaterials for nanomedicine applications

Multimodal Microscopy Distinguishes Extracellular Aggregation and Cellular Uptake of Single‐Walled Carbon Nanohorns

Contact Info

Product

Resources

About