Evaluation of the biological and physicochemical properties of calcium silicate‐based and epoxy resin‐based root canal sealers

Ferreira, Gabriela Cardoso; Pinheiro, Lucas Siqueira; Nunes, Júlia Silveira; Mendes, Roberta Almeida; Schuster, Cláudia Daniela; Soares, Renata Grazziotin; Kopper, Patrícia Maria Poli; Figueiredo, José Antônio Poli de; Grecca, Fabiana Soares

doi:10.1002/jbm.b.35004

Cited by 9 publications

(8 citation statements)

References 32 publications

(78 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, EDS only exhibits the distribution of elements on the sample's surface. Other complementary techniques such as X-ray diffraction (XRD) analysis or attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy are needed to identify calcium hydroxide peaks and other crystalline phases in hydraulic cements after setting (Ferreira et al, 2021).…”

Section: F I G U R Ementioning

confidence: 99%

“…Therefore, sealers should exhibit an adequate biocompatibility, i.e. they should not induce an adverse reaction or response from biological tissues upon contact (Ferreira et al, 2021). The same applies on a cellular level, wherein surrounding cellular populations should not experience a decrease in their viability, migration/proliferation or differentiation (da Silva et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

et al. 2022

View full text Add to dashboard Cite

Aim To assess the cytocompatibility and bioactive potential of the new calcium silicate cement‐based sealer AH Plus Bioceramic Sealer (AHPbcs) on human periodontal ligament stem cells (hPDLSCs) compared with the epoxy resin‐based sealer AH Plus (AHP) and the calcium silicate cement‐based sealer Endosequence BC Sealer (ESbcs). Methodology Standardized sample discs and 1:1, 1:2 and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM–EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, cell migration/proliferation via a wound‐healing assay, osteo/cemento/odontogenic marker expression via RT‐qPCR and cell mineralized nodule formation via Alizarin Red S staining. HPDLSCs were isolated from extracted third molars. Comparisons were made with hPDLSCs cultured in unconditioned (negative control) or osteogenic (positive control) culture media. Statistical significance was established at p < .05. Results A higher peak of Ca2+ was detected from ESbcs compared with AHPbcs and AHP in SEM–EDX. Both AHPbcs and ESbcs showed significantly positive results in the cytocompatibility assays (cell viability, migration/proliferation, attachment and morphology) compared with a negative control group, whilst AHP showed significant negative results. Both AHPbcs and ESbcs exhibited an upregulation of at least one osteo/odonto/cementogenic marker compared with the negative and positive control groups. Both ESbcs and AHPbcs showed a significantly higher calcified nodule formation than the negative and positive control groups, indicative of their biomineralization potential and were also significantly higher than AHP group. Conclusion AH Plus Bioceramic Sealer exhibited a significantly higher cytocompatibility and bioactive potential than AH Plus and a similar cytocompatibility to that of Endosequence BC Sealer. Endosequence BC Sealer exhibited a significantly higher mineralization potential than the other tested sealers. The results from this in vitro study act as supporting evidence for the use of AH Plus Bioceramic Sealer in root canal treatment.

show abstract

Section: F I G U R Ementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Three of the six studies that analyzed the response in the dorsal subcutaneous tissue of rats (inflammatory infiltrate) also assessed cell viability. [ 13 39 49 ] Forty-one studies included in this systematic review investigated only cell viability. [ 1 2 3 9 10 11 12 14 15 27 28 29 30 31 32 33 34 35 37 38 40 41 43 44 45 46 47 48 50 51 52 53 55 56 57 60 61 62 63 65 ] In addition, four studies that investigated genotoxicity also analyzed cell viability[ 4 54 58 64 ] and one study investigated only genotoxicity.…”

Section: Resultsmentioning

confidence: 99%

“…The biological properties of premixed calcium silicate-based root canal sealers have been widely described. [ 4 13 14 15 16 17 ] However, no previous meta-analysis has been conducted to integrate the results of these studies and test the hypothesis that premixed calcium silicate-based sealers have better biological properties than the “gold standard” AH Plus. The present study tested this alternative hypothesis and the results suggest that the new bioceramic sealers have better response in the dorsal subcutaneous tissue of rats and lower cytotoxicity than AH Plus, whereas no difference was found with regard to genotoxicity as indicated by MNT.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Premixed calcium silicate-based root canal sealers have better biological properties than AH Plus: A systematic review and meta-analysis of in vivo animal studies and in vitro laboratory studies

Malta,

Santi,

Barcelos

et al. 2024

Journal of Conservative Dentistry and Endodontics

View full text Add to dashboard Cite

Objectives: The aim was to determine whether premixed calcium silicate-based root canal sealers have better biological properties than AH Plus. Materials and Methods: Searches of studies published up to January 2023 were performed in the PubMed/MEDLINE and EMBASE and via other methods (databases of the International Endodontic Journal, Journal of Endodontics, and gray literature). The inclusion criteria were in vivo animal and in vitro studies that analyzed the response in the dorsal subcutaneous tissue of rats, cell viability, and genotoxicity. Systematic Review Centre for Laboratory Animal Experimentation Risk of Bias (RoB) tool for in vivo studies and modified CONSORT checklist for in vitro were appraised. Meta-analysis was performed using the Stata. Results: Fifty-two studies were included. In the RoB, in vivo studies fulfilled 20%–50% of the items and in vitro 60%–100%. The studies included in the meta-analysis demonstrated better histocompatibility with the premixed calcium silicate-based sealers at 30 days and greater cell viability with these sealers when used in undiluted extracts in experimental period of 72 h and in extracts with 1:2 and 1:4 dilution in 24 and 72 h. In contrast, no difference between materials was found concerning genotoxicity. Conclusion: Premixed calcium silicate-based root canal sealers have better histocompatibility and are less cytotoxic than the epoxy resin-based sealer AH Plus, demonstrating favorable biological behavior.

show abstract

Biological properties of Ceraputty as a retrograde filling material: an in vitro study on hPDLSCs

et al. 2023

View full text Add to dashboard Cite

Objectives To assess the cytocompatibility and bioactive potential of the new calcium silicate-based cement Ceraputty on human periodontal ligament stem cells (hPDLSCs) compared to Biodentine and Endosequence BC root repair material (ERRM). Materials and methods hPDLSCs were isolated from extracted third molars from healthy donors. Standardized sample discs and 1:1, 1:2, and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM–EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, osteo/cemento/odontogenic marker expression via RT-qPCR, and cell calcified nodule formation via Alizarin Red S staining. hPDLSCs cultured in unconditioned or osteogenic media were used as negative and positive control groups, respectively. Statistical analysis was performed using one-way ANOVA or two-way ANOVA and Tukey’s post hoc test. Statistical significance was established at p < 0.05. Results The highest Ca2+ peak was detected from Biodentine samples, followed by ERRM and Ceraputty. hPDLSC viability was significantly reduced in Ceraputty samples (p < 0.001), while 1:2 and 1:4 Biodentine and ERRM samples similar results to that of the negative control (p > 0.05). Biodentine and ERRM exhibited an upregulation of at least one cemento/odonto/osteogenic marker compared to the negative and positive control groups. Cells cultured with Biodentine produced a significantly higher calcified nodule formation than ERRM and Ceraputty (p < 0.001), which were also higher than the control groups (p < 0.001). Conclusion Ceraputty evidenced a reduced cytocompatibility towards hPDLSCs on its lowest dilutions compared to the other tested cements and the control group. Biodentine and ERRM promoted a significantly higher mineralization and osteo/cementogenic marker expression on hPDLSCs compared with Ceraputty. Further studies are necessary to verify the biological properties of this new material and its adequacy as a retrograde filling material. Clinical relevance This is the first study to elucidate the adequate biological properties of Ceraputty for its use as a retrograde filling material.

show abstract

Evaluation of the biological and physicochemical properties of calcium silicate‐based and epoxy resin‐based root canal sealers

Cited by 9 publications

References 32 publications

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

Premixed calcium silicate-based root canal sealers have better biological properties than AH Plus: A systematic review and meta-analysis of in vivo animal studies and in vitro laboratory studies

Biological properties of Ceraputty as a retrograde filling material: an in vitro study on hPDLSCs

Contact Info

Product

Resources

About