Evaluation of the biocompatibility of resin composite‐based dental materials with gingival mesenchymal stromal cells

Abstract

Resin composite‐based dental materials can leach certain components into the oral environment, causing potentially harmful gingival biological effect. Gingival tissue is a rich source of mesenchymal stem cells (MSCs) that is easily accessible, and can be used as a complementary approach for the investigation of dental material biocompatibility. Using gingival MSCs (gMSCs), the present study aimed to investigate the cytotoxicity of two classes of restorative dental materials (ormocers and resin composites) used to restore class II cavities close to the gingival margin, in addition to analyzing the leached compounds from these resin composite‐based materials. Functionality assays (Colony‐forming unit, migratory potential, and proliferation assays) and a viability assay (MTT) were employed. Cells' aspect was observed by scanning electron microscopy (SEM). Leached monomers were also quantitated using high‐performance liquid chromatography (HPLC). The cytotoxicity of the biomaterials was highlighted by impaired functionality and diminished viability of gMSCs. Despite being variants of the same commercial material, the two ormocers behaved differently one material having a more negative impact on cell functionality than the other. Cells appeared to attach well to all materials. Main monomer molecules were mostly released by the tested materials. For all samples, an increased elution of monomers was recorded in artificial saliva as compared with culture medium. One composite material has released nearly eight times more urethane dimetacrylate in artificial saliva than in culture medium. Significantly lower gMSC viability scores were recorded for all the investigated samples in comparison with the control.

Are Metal Ions That Make up Orthodontic Alloys Cytotoxic, and Do They Induce Oxidative Stress in a Yeast Cell Model?

“…Although several studies are available in the literature regarding the biocompatibility and potential cytotoxic impact of the metal ions released from implants [21][22][23], restorations [24][25][26] and orthodontic appliances such as Fe, Ni, Cr, Co, Ti, and Mo [27][28][29], no previous report has simulated orthodontic alloys with metal ion mixtures to evaluate their potential. Cultured animal or human cell lines such as fibroblasts [28] and osteoblasts [30] have been mainly used to evaluate the cytotoxic effect of orthodontic appliances, and currently, only a few studies [31,32] have included yeast for these type of studies.…”

[Retracted] Implicit Environmental Injustice in Global Trade: Based on the MRIO Model

“…Although the China-ASEAN Free Trade Area is a joint venture project between China and the ten ASEAN countries, the trade volume between China and the ten ASEAN countries is different. As can be seen from Figure 5 , the average trade volume between China and ASEAN countries from 2010 to 2017 was 16% in Brunei, 25% in Myanmar, 48% in Cambodia, and 11% in Laos [ 13 , 14 ].…”

In Vitro Study on the Adhesive Performance of Some Resin-Based Materials Used to Restore Class II Cavities

“…The relative equal performance of the materials may be due to the fact that the first generation of ormocer materials used in this study contain conventional methacrylate diluents, which may prevent to fulfil the expected potential [ 33 , 34 ], but similar shear bond strength [ 35 ], in vivo sealing ability and polymerization shrinkage are used [ 11 ]. The very recent dimethacrylate-diluent-free ormocer matrices seemed to improve ormocer behavior [ 7 ].…”