Evaluation of the Beckman Coulter UniCel DxH 800, Beckman Coulter LH 780, and Abbott Diagnostics Cell-Dyn Sapphire Hematology Analyzers on Adult Specimens in a Tertiary Care Hospital

Abstract

We evaluated the new Beckman Coulter DxH 800 hematology analyzer (Beckman Coulter, Miami, FL) vs the Abbott Diagnostics Cell-Dyn Sapphire (Abbott Diagnostics, Santa Clara, CA) and Beckman Coulter LH 780 hematology analyzers using 430 adult specimens. The DxH 800 provided a CBC and differential that correlated well with those of the Sapphire and LH 780, with most parameters showing correlation coefficients (r) of more than 0.97. In the instrument vs 400-cell manual differential comparison, all 3 instruments showed similar and acceptable accuracy to the reference method except for nucleated RBC (NRBC) enumeration, in which the DxH 800 and Sapphire outperformed the LH 780. We also compared clinical efficiency by determining whether flagged specimens showed abnormalities on a peripheral blood smear as defined by International Council for Standardization in Haematology criteria. The efficiency, sensitivity, and specificity of the DxH 800 were 77.0%, 87.1%, and 73.0%, respectively, compared with the Sapphire at 75.8%, 93.5%, and 68.8%, respectively, and LH 780 at 66.1%, 93.5%, and 55.3%, respectively.

The HemoScreen, a novel haematology analyser for the point of care

“…In the differential a high correlation (r>0.970) was demonstrated for NEUT, LYMP and EOS, whereas a moderate correlation (r=0.823) was found for MONO and low correlation (r=0.447) was found for BASO. These findings are equivalent to those observed for other comparisons of state-of-the-art laboratory analysers 19 20…”

A new self-partition clustering method for robust identification of subsets with heterogeneous size and density and its clinical application to leukocyte differential counting

“…Whereas, the discrepancy of manual monocyte counts from those by SPC/ITC method and by the hematology analyzer requires special attention. In the literature, similar observations of discrepancy were reported for monocyte counts between manual and automated counting [17,18]. They were partly attributed to the presence of immature small monocytes, which were not counted as monocytes, or the presence of atypical lymphocytes, which were counted as monocytes.…”

Point-of-Care Versus Central Laboratory Measurements of Hemoglobin, Hematocrit, Glucose, Bicarbonate and Electrolytes: A Prospective Observational Study in Critically Ill Patients

“…Although the reference method for hemoglobinometry is spectrophotometry using hemiglobin-cyanide, cyanide-free reagents have been widely adopted for safety and environmental purpose [34, 35]. Therefore, the DxH 800 analyzer represents current standards [36, 37]. We found that hemoglobin was systematically overestimated (mean 0.8 g/dL) by the POC assay.…”