Evaluation of the antiparkinsonism and neuroprotective effects of hydrogen sulfide in acute 6-hydroxydopamine-induced animal model of Parkinson’s disease: behavioral, histological and biochemical studies

Sarukhani, Mohammad; Haghdoost-Yazdi, Hashem; Golezari, Ali Sarbazi; Babayan-Tazehkand, Arvin; Dargahi, Tahereh; Rastgoo, Nafiseh

doi:10.1080/01616412.2017.1390903

Cited by 26 publications

(10 citation statements)

References 31 publications

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“…Our study uses NaHS as the donor of H 2 S, belonging to the follower of the first model which has quite low relevance to a physiological situation. NaHS, an H 2 S donor, was found to protect PC12 cells against MPP~+-induced apoptosis and cytotoxicity [23]. First of all, we found that H 2 S protected SH-SY5Y cells induced by MPP~+ through increasing autophagy flux.…”

Section: Discussionmentioning

confidence: 52%

NaSH increases SIRT1 activity and autophagy flux through sulfhydration to protect SH-SY5Y cells induced by MPP~+

Wang

et al. 2020

Cell Cycle

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Parkinson's disease (PD) is one of the most prevailing aging diseases around the world. The present study was to investigate the potential effect of hydrogen sulfide (H 2 S) and silent mating type information regulation 2 homolog 1 (SIRT1) in MPP~+ induced SH-SY5Y cells and its underlying mechanisms in PD. SH-SY5Y cells were induced by MPP~+ and treated with the H 2 S donor NaHS to detect the effect of H 2 S on the molecular behaviors of MPP~+ induced SH-SY5Y cells. NaHS reduced the apoptosis rate and expressions of MDA, 4-HNE and p62, while increased cell viability, autophagy flux and expressions of LC3 II/I and Beclin1 in MPP~+ induced SH-SY5Y cells. Then, levels of autophagy-related proteins and inflammation-related proteins (TNF-α, IL-Iβ) were detected, indicating that Chloroquine and Sirtinol reversed the protective effect of H 2 S on SH-SY5Y cells induced by MPP~+. We further explored the particular function of H 2 S, SH-SY5Y cells treated with MPP~+, NaHS chloroquine, and SIRT1 inhibitor (Sirtinol). The results showed that H 2 S increased SIRT1 expression and sulfhydration. Finally, a PD mouse model verified the above results. In a word, H 2 S ameliorated SIRT1 activity through acceleration of SIRT1 sulfhydration to increase the autophagy flux and attenuate damage of SH-SY5Y cells induced by MPP~+. H 2 S and SIRT1 activator might be a target in the treatment of PD patients.

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Section: Discussionmentioning

confidence: 52%

NaSH increases SIRT1 activity and autophagy flux through sulfhydration to protect SH-SY5Y cells induced by MPP~+

Wang

et al. 2020

Cell Cycle

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“…Motor coordination and balance of rats were tested with rotarod test (Rota-Rod ENV-578(R), MedAssociates) as previously described (24). Briefly, rats were placed on a rotative axis and their latency to fall was measured.…”

Section: Rotarod Testmentioning

confidence: 99%

Preventive Vitamin A Supplementation Improves Striatal Function in 6-Hydroxydopamine Hemiparkinsonian Rats

et al. 2022

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BackgroundThe mechanisms leading to a loss of dopaminergic (DA) neurons from the substantia nigra pars compacta (SNc) in Parkinson's disease (PD) have multifactorial origins. In this context, nutrition is currently investigated as a modifiable environmental factor for the prevention of PD. In particular, initial studies revealed the deleterious consequences of vitamin A signaling failure on dopamine-related motor behaviors. However, the potential of vitamin A supplementation itself to prevent neurodegeneration has not been established yet.ObjectiveThe hypothesis tested in this study is that preventive vitamin A supplementation can protect DA neurons in a rat model of PD.MethodsThe impact of a 5-week preventive supplementation with vitamin A (20 IU/g of diet) was measured on motor and neurobiological alterations induced by 6-hydroxydopamine (6-OHDA) unilateral injections in the striatum of rats. Rotarod, step test and cylinder tests were performed up to 3 weeks after the lesion. Post-mortem analyses (retinol and monoamines dosages, western blots, immunofluorescence) were performed to investigate neurobiological processes.ResultsVitamin A supplementation improved voluntary movements in the cylinder test. In 6-OHDA lesioned rats, a marked decrease of dopamine levels in striatum homogenates was measured. Tyrosine hydroxylase labeling in the SNc and in the striatum was significantly decreased by 6-OHDA injection, without effect of vitamin A. By contrast, vitamin A supplementation increased striatal expression of D2 and RXR receptors in the striatum of 6-OHDA lesioned rats.ConclusionsVitamin A supplementation partially alleviates motor alterations and improved striatal function, revealing a possible beneficial preventive approach for PD.

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“…1 In the last 10 years, a large amount of evidence has confirmed that the neuronal apoptosis of dopaminergic neurons in the substantia nigra is implicated as the main mechanism of neuronal death in the development of PD. 2,3 Developing treatments to slow or prevent the neuronal apoptosis in the substantia nigra of PD is a challenge and a goal worthy of further research.…”

Section: Introductionmentioning

confidence: 99%

“…4 Recently, emerging research reveals that the inhibition of neuronal apoptosis in the substantia nigra by H 2 S is of great significance in antagonising PD. 2,5,6 However, the mechanism underlying the protection of H 2 S against neuronal apoptosis in PD remains undefined. Autophagy, a process in which lysosomes degrade cell organelles and proteins, is a pivotal participant for the circulation of cellular components, the maintenance of intracellular homeostasis, and the initiation of cell death programs.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen sulphide attenuates neuronal apoptosis of substantia nigra by re‐establishing autophagic flux via promoting leptin signalling in a 6‐hydroxydopamine rat model of Parkinson's disease

Zou

et al. 2021

Clin Exp Pharma Physio

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Previous studies reveal that hydrogen sulphide (H 2 S) exerts neuroprotection against neurotoxin-induced Parkinson's disease (PD), but the underlying mechanism remains elusive. The present study was aimed to investigate whether H 2 S inhibits neuronal apoptosis of substantia nigra with the involvement of autophagy via promoting leptin signalling in 6-hydroxydopamine (6-OHDA)-induced PD rats. In this study, neuronal apoptosis was analysed by TUNEL staining, the activity of caspase-3 was measured by Caspase-3 fluorometric assay kit, the expressions of Bax, Bcl-2, Beclin-1, LC3II, P62 and leptin were determined by Western blot analysis, and the numbers of autophagosomes and autolysosomes were assessed by transmission electron microscopy.Results showed that NaHS, a donor of exogenous H 2 S, mitigates 6-OHDA-induced the increases in the numbers of TUNEL-positive cells, the activity of caspase-3 and the expression of Bax, and attenuates 6-OHDA-induced a decrease in the expression of Bcl-2 in substantia nigra of rats. In addition, 6-OHDA enhanced the expressions of Beclin-1, LC3-II and P62, increased the number of autophagosomes, and decreased the number of autolysosomes in the substantia nigra, which were also blocked by administration of NaHS. Furthermore, NaHS reversed 6-OHDA-induced the downregulation of leptin expression in the substantia nigra, and treatment with leptin-OBR, a blocking antibody of leptin receptor, attenuated the inhibition of NaHS on neuronal apoptosis and the improvement of NaHS on the blocked autophagic flux in substantia nigra of 6-OHDA-treated rats. Taken together, these results demonstrated that H 2 S attenuates neuronal apoptosis of substantia nigra depending on restoring impaired autophagic flux through up-regulating leptin signalling in PD.

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Evaluation of the antiparkinsonism and neuroprotective effects of hydrogen sulfide in acute 6-hydroxydopamine-induced animal model of Parkinson’s disease: behavioral, histological and biochemical studies

Cited by 26 publications

References 31 publications

NaSH increases SIRT1 activity and autophagy flux through sulfhydration to protect SH-SY5Y cells induced by MPP~+

NaSH increases SIRT1 activity and autophagy flux through sulfhydration to protect SH-SY5Y cells induced by MPP~+

Preventive Vitamin A Supplementation Improves Striatal Function in 6-Hydroxydopamine Hemiparkinsonian Rats

Hydrogen sulphide attenuates neuronal apoptosis of substantia nigra by re‐establishing autophagic flux via promoting leptin signalling in a 6‐hydroxydopamine rat model of Parkinson's disease

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