Evaluation of the antioxidant properties of Vitis vinifera juice extract in rifampicin dispersions

Abstract

The purpose of this study was to evaluate the antioxidant properties of Vitis vinifera juice extract (grape) in rifampicin suspensions and to compare with that of ascorbic acid. Grape juice was extracted and used as antioxidant in both pure sample and commercial reconstituted samples of rifampicin. The rate of drug degradation was determined by spectroscopic method in a time dependent manner. Also, the antioxidant property of grape juice was compared with that of ascorbic acid. Phytochemical screening of the constituents in grape juice extract was carried out according to standard methods. The results revealed that rifampicin dispersions containing ascorbic acid (batches A1 to A5) showed significantly higher stability and had higher amount of drug remaining over time than the control (batch A6) containing no antioxidant (p < 0.05). However, the results revealed that grape fruit juice extract had higher antioxidant properties than the ascorbic acid (p < 0.05) in rifampicin dispersions. The results also indicated that increase in amount of both antioxidants significantly reduced the rate of drug degradation through oxidation, thereby causing a corresponding increase in the amount of rifampicin remaining over time. Grape juice extract contain alkaloids, carbohydrates, saponins, reducing sugars, steroids, tannins, proteins, flavonoids, resins, oils, terpenoids and acid compounds. Glycosides were however not found in the juice extract. Therefore, grape fruit juice extract could be used as a natural antioxidant in rifampicin suspensions.

„Azon orvosságok kezdete, melyeket a májra adnak”

“…Biliaris dyspepsiában, haemorrhagiában, dysuriában és krónikus bronchitisben, valamint szívelégtelenségben és köszvény kezelésében jelenleg is használatos. Az érett gyümölcs vagy a mazsola a szubakut lép-és májmegnagyobbodás kezelésében bizonyult hatásosnak [26]. A Ceratonia siliqua L. (Fabaceae), a szentjánoskenyér, csak a fenti (Eb480) májpanaszok kezelésére szánt óegyiptomi orvosi receptben szerepel.…”

An Allometric Growth Estimation of Grapes (&lt;i&gt;Vitis vinifera&lt;/i&gt; L) from High Mountainous Region of Gilgit-Baltistan, Pakistan

“…In addition to it, the grape contains significant amounts of potassium 0.1-0.2%, vitamin C 0.01-0.02%, and vitamin A 0.001-0.0015% [12]. Dried grapes are very fruitful in thirst on fevers, cough, inflammation of the mucous membranes of the nose and throat, jaundice, and in sub-acute cases of enlarged liver and spleen [13].…”