Evaluation of the antioxidant effects in vitro of the isopulegone

Abstract

Background: Studies report that monoterpenes and their synthetic derivatives have diverse biological and pharmacological properties. Among these compounds, isopulegone present in the essential oil of various aromatic plants have been used in the production of cosmetics. Objectives: evaluated the antioxidant properties of (-)-isopulegone in vitro against the formation of reactive species using the tests thiobarbituric acid (TBARS), hydroxyl radical and nitric oxide production. Materials and Methods: The (-)-isopulegone was tested at different concentrations (0.9, 1.8, 3.6, 5.4, 7.2 µg/mL), and compared with trolox (positive control). We evaluated the level of lipid peroxidation induced by 2,2'-azobis-2methyl-propanimidamide dihydrochloride (AAPH) by the amount of TBARS formed. We evaluated the in vitro effects of (-)-isopulegone against hydroxyl radical formation from Fenton reaction. In assessing production of nitric oxide, was used sodium nitroprusside on decomposition. Results: (-)-Isopulegone in the concentrations tested, was capable of preventing lipid peroxidation induced by AAPH, reducing the amount of TBARS formed. The hydroxyl radical formation was inhibited, suggesting a possible antioxidant action of this monoterpene. In the evaluation of the production of nitric oxide isopulegone decreased the formation of such compound, showing a shield in vitro profile of biomolecules such as lipids of the cell membranes from damage caused by free radicals generated. The (-)-isopulegone demonstrated strong antioxidant potential in vitro, by removal capacity against hydroxyl radicals and nitric oxide, and prevented the formation of TBARS, similarly to trolox (positive control). Conclusion: However, further studies are needed to better characterize the antioxidant properties of (-)-isopulegone, especially prominently in vitro.

Production and composition of peppermint essential oil in seasons after summer and winter transplantations

“…According to the authors, the presence of pulegone is directly related to the antifungal properties of mint. Another major component found in mint essential oil was isopulegone, which demonstrated good antioxidant potential in a study carried out in vitro, reducing free radicals (Silva et al, 2012).…”

Comparative antioxidant, antibacterial and phytochemical analysis of roots, stems, leaves and seeds from <i>Cleome rutidosperma</i> DC

“…Plant-derived antioxidants guard against oxidative stress by discarding ROS and RNS (reactive nitrogen species). They also suppress the polymerization chain reaction that free radicals initiate (Ahmed et al, 2015;Bianco & Uccella, 2000;Céspedes et al, 2008;Lafka et al, 2007;O.A. Silva et al, 2012).…”

In vitro Antioxidant Properties of Underutilized Baccaurea angulata Fruit

“…HE toxicity concern of synthetic antioxidants such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT), which are being widely used as food additives to prevent oxidative deterioration of food products [1] has been generating endless controversies and debates among nutrionists and dieticians. Medicinal and aromatic plants, as alternatives, are known to produce a variety of antioxidants, which can militate against cellular oxidative damage by removing reactive oxygen species (ROS) and reactive nitrogen species (RNS), both in food and human body, through the delay of the oxidation process as well as inhibition of the polymerization chain reaction initiated by free radicals [2][3][4][5]. Several epidemiologic studies have also established the fact that high polyphenol intake from fruits and vegetables are associated with decreased risk for cardiovascular disease and other developing degenerative diseases [6,7].…”