Abstract:A polyphenolic extract of Robinia pseudoacacia L. flowers was prepared using a hydro-ethanolic extraction phase. The chemical analysis of the extract consisted in the assessment of the total polyphenolic content (Folin-Ciocalteu method) and the flavonoid fingerprint of the extract determined by high-performance thin-layer chromatography (HPTLC). Two methods ferric reducing antioxidant power (FRAP) and radical scavenging activity with DPPH were used to measure the antioxidant activity of the extract. The cytoto… Show more
“…They concluded that the 70% ( v / v ) methanolic and 80% ( v / v ) ethanolic extracts did not show cytotoxic activity against Vero African green monkey kidney cells because the IC 50 values (in that paper, IC 50 is presented as the LC 50 value) were higher than 1 mg/mL. Bratu et al [ 33 ] also confirmed the cytotoxic activity of 50% ( v / v ) ethanolic extract of black locust flower prepared via maceration against HeLa cells. That extract did not express the cytotoxic effect against the palatal mesenchymal stem cells.…”
Black locust flower extract contains various polyphenols and their glucosides contribute to the potential health benefits. After intake of these bioactive compounds and passage through the gastrointestinal tract, their degradation can occur and lead to a loss of biological activity. To overcome this problem, the bioactive compounds should be protected from environmental conditions. This study aimed to encapsulate the black flower extract in the microparticles based on biodegradable polysaccharides, alginate, and chitosan. In the extract, the total antioxidant content was found to be 3.18 ± 0.01 g gallic acid equivalent per 100 g of dry weight. Also, the presence of lipids (16), phenolics (27), organic acids (4), L-aspartic acid derivative, questinol, gibberellic acid, sterol, and saponins (2) was confirmed using the UHPLC–ESI–MS analysis. In vitro assays showed that the extract has weak anti-α-glucosidase activity and moderate antioxidant and cytotoxic activity against the HeLa cell line. The extrusion method with secondary air flow enabled the preparation of microparticles (about 270 μm) encapsulated with extract. An encapsulation efficiency of over 92% was achieved in the alginate and alginate–chitosan microparticles. The swelling study confirmed a lower permeability of alginate–chitosan microparticles compared with alginate microparticles. For both types of microparticles, the release profile of antioxidants in the simulated gastrointestinal fluids at 37 °C followed the Korsmeyer–Peppas model. A lower diffusion coefficient than 0.5 indicated the simple Fick diffusion of antioxidants. The alginate–chitosan microparticles enabled a more sustained release of antioxidants from extract compared to the alginate microparticles. The obtained results indicated an improvement in the antioxidant activity of bioactive compounds from the extract and their protection from degradation in the simulated gastric conditions via encapsulation in the polymer matrixes. Alginate–chitosan showed slightly slower cumulative antioxidant release from microparticles and better antioxidant activity of the extract compared to the alginate system. According to these results, alginate–chitosan microparticles are more suitable for further application in the encapsulation of black locust flower extract. Also, the proposed polymer matrix as a drug delivery system is safe for human use due to its biodegradability and non-toxicity.
“…They concluded that the 70% ( v / v ) methanolic and 80% ( v / v ) ethanolic extracts did not show cytotoxic activity against Vero African green monkey kidney cells because the IC 50 values (in that paper, IC 50 is presented as the LC 50 value) were higher than 1 mg/mL. Bratu et al [ 33 ] also confirmed the cytotoxic activity of 50% ( v / v ) ethanolic extract of black locust flower prepared via maceration against HeLa cells. That extract did not express the cytotoxic effect against the palatal mesenchymal stem cells.…”
Black locust flower extract contains various polyphenols and their glucosides contribute to the potential health benefits. After intake of these bioactive compounds and passage through the gastrointestinal tract, their degradation can occur and lead to a loss of biological activity. To overcome this problem, the bioactive compounds should be protected from environmental conditions. This study aimed to encapsulate the black flower extract in the microparticles based on biodegradable polysaccharides, alginate, and chitosan. In the extract, the total antioxidant content was found to be 3.18 ± 0.01 g gallic acid equivalent per 100 g of dry weight. Also, the presence of lipids (16), phenolics (27), organic acids (4), L-aspartic acid derivative, questinol, gibberellic acid, sterol, and saponins (2) was confirmed using the UHPLC–ESI–MS analysis. In vitro assays showed that the extract has weak anti-α-glucosidase activity and moderate antioxidant and cytotoxic activity against the HeLa cell line. The extrusion method with secondary air flow enabled the preparation of microparticles (about 270 μm) encapsulated with extract. An encapsulation efficiency of over 92% was achieved in the alginate and alginate–chitosan microparticles. The swelling study confirmed a lower permeability of alginate–chitosan microparticles compared with alginate microparticles. For both types of microparticles, the release profile of antioxidants in the simulated gastrointestinal fluids at 37 °C followed the Korsmeyer–Peppas model. A lower diffusion coefficient than 0.5 indicated the simple Fick diffusion of antioxidants. The alginate–chitosan microparticles enabled a more sustained release of antioxidants from extract compared to the alginate microparticles. The obtained results indicated an improvement in the antioxidant activity of bioactive compounds from the extract and their protection from degradation in the simulated gastric conditions via encapsulation in the polymer matrixes. Alginate–chitosan showed slightly slower cumulative antioxidant release from microparticles and better antioxidant activity of the extract compared to the alginate system. According to these results, alginate–chitosan microparticles are more suitable for further application in the encapsulation of black locust flower extract. Also, the proposed polymer matrix as a drug delivery system is safe for human use due to its biodegradability and non-toxicity.
“…Linalool, cis-β-ocimene, methyl anthranilate, phenyl ethyl alcohol, germacrene D, (E)-α-bergamotene, benzeneacetic acid methyl ester, (Z)-nerolidol, and indole are important contributors to the pleasant aroma of the flowers of R. pseudacacia [ 125 ]. The black locust flower polyphenolic extract contains a significant percentage of polyphenolic compounds and presents good antioxidant and antitumoral activity [ 126 ]. R. pseudacacia flowers are used in Italy as an infusion for the treatment of flu or as sedative [ 24 , 39 ], while in Turkey the infusion is used as a generic product which is good for health [ 127 ].…”
Edible flowers are becoming an essential component of people’s nutrition in the Mediterranean basin. In the last decades, many researchers also have focused their attention on the nutritional composition of the edible flowers, as well as their antioxidant and antimicrobial properties, including studies on their safety issues. Despite the growing interest in the use of flowers in human nutrition, the ethnobotanical literature is lacking coverage of some important issues, particularly those which concern the use of flowers in the folk tradition. Only recently, a review regarding the contribution of 32 edible flowers to the Mediterranean diet was published. The aim of the present review is to document the plant lore regarding the wild and cultivated edible flowers consumed in the Mediterranean basin. Based on the 112 studies reviewed, we documented 251 taxa as being used in the Mediterranean basin as edible flowers. The plant species belong to 45 families and 141 genera. Asteraceae (54 taxa) is the most frequently cited family. Sambucus nigra L. is the most cited species. This study can be the basis for future research on the supposed bioactivity and toxicity of wild and cultivated flowers.
“…During experiments, we were able to prove the increased antioxidant activity, as well as the in vitro antitumor effect of polyphenolic extracts of Robinia pseudoacacia flowers [12].…”
Section: Introductionmentioning
confidence: 95%
“…The mobile phase consisted of ethyl acetate-acetic acidformic acid-water 100:11:11:27 (v/v/v/v); as stationary phase pre-coated silica gel 60 F254 HPTLC plates were used (20 cm x 10 cm, Merck, Darmstadt, Germany). The sample application technique and the development mode were carried out according to the method described by Bratu et al, [12]. After development, the plates were dried at 105ºC for 10 min and derivatised in NP-PEG reagents [19].…”
Section: Antioxidant Activitymentioning
confidence: 99%
“…Cytotoxic and apoptosis effects were determined according to the method described by Lazarević et al, 2019 [22] and Bratu et al, 2021 [12]. Thus, for the cytotoxic assay, two cells' lines were used: fully characterised palatal mesenchymal stem cells isolated from human oral cavity (pMSCs), and epithelial cell line derived from human cervical adenocarcinoma (HeLa ATTC® CCL-2™).…”
Section: Estimation Of Cytotoxic and Antitumor Effectsmentioning
Helichrysum arenarium (L) Moench (sandy everlasting) is a valuable plant for therapy, having multiple applications in traditional medicine, applications which are confirmed nowadays by science. The main polyphenols found in sandy everlasting flowers were shown to have antioxidant and UVscreen effects.In order to expand our knowledge concerning the synergistic therapeutic effects of the polyphenols from Helichrysum arenarium (L) flowers, an extract was prepared using 50% ethanol as extraction phase, followed by the removing of ethanol using a Royeyov IKA RV10 rotary evaporator. The chemical analysis of the extract consisted in the assessment of total polyphenol content (Folin-Ciocalteu) and flavonoid fingerprint (high-performance thin-layer chromatography -HPTLC).For the polyphenolic extract there were assessed the antioxidant activity (measured by two methods: ferric reducing antioxidant power (FRAP) and radical scavenging activity with DPPH)) and sun protection factor (SPF) (assessed using an in vitro spectrometric method).Furthermore, the cytotoxic and antitumor effects were evaluated on two cell lines: pMSCs (palatal mesenchymal stem cells) and HeLa (ATTC® CCL-2™) cell line (epithelial cells derived from
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